Q9CIT4 (ACMA_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable N-acetylmuramidase
EC=3.2.1.17

Alternative name(s):
Autolysin
Lysozyme
Peptidoglycan hydrolase

Gene names

Name:	acmA
Ordered Locus Names:	LL0272
ORF Names:	L68758

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for cell separation during growth By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Secreted By similarity.
Domain	The LysM repeats are thought to be involved in peptidoglycan binding.
Sequence similarities	Belongs to the glycosyl hydrolase 73 family. Contains 3 LysM repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	amidase activity Inferred from electronic annotation. Source: InterPro lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 57	57	Potential
	Chain	58 – 439	382	Probable N-acetylmuramidase		PRO_0000012112
Regions
	Repeat	243 – 285	43	LysM 1
	Repeat	323 – 365	43	LysM 2
	Repeat	397 – 439	43	LysM 3
	Compositional bias	287 – 291	5	Poly-Ser
	Compositional bias	370 – 374	5	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q9CIT4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: AE4551E4D04CB499	FASTA	439	46,592
	10 20 30 40 50 60 MPVSRIKVKN RHLKKKAKKP LAFYKPATKF AGAVLIAGTL TTTHELLLQQ TSPMVQAATN 70 80 90 100 110 120 STEAFIESIA ASAKPVADSN GLYPSVMIAQ AILESNWGSS QLSRAPYYNL FGIQGTYQGK 130 140 150 160 170 180 SVVFKTQEYL NGKWVTKDMP FRVYPSFNQS FQDNAYVLKT TNFGNGPYYA KAWRANAATY 190 200 210 220 230 240 QAATAALTGK YATDPNYGAS LNRIISQYNL TRFDGASSAG TSNSGGSTAT NTNNNSNTSS 250 260 270 280 290 300 TTYTVKSGDT LWGISQKYGI SVAQIQSANN LKSTVIYIGQ KLVLTTSSSS SNTNSSTSSG 310 320 330 340 350 360 NSAGTTTPTT SVTPAKPASQ TTIKVKSGDT LWGLSVKYKT TIAQLKSWNH LNSDTIFIGQ 370 380 390 400 410 420 NLIVSQSAGS SSSSTGSSSA STSSTSNSSA ASNTSIHKVV KGDTLWGLSQ KSGSPIASIK 430 AWNHLSSDTI LIGQYLRIK

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04370.1.
PIR	H86658.
RefSeq	NP_266428.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CIT4.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L68758.
Protein family/group databases
CAZy	CBM50. Carbohydrate-Binding Module Family 50. GH73. Glycoside Hydrolase Family 73.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK04370; AAK04370; L68758.
GeneID	1113882.
KEGG	lla:L68758.
PATRIC	22292728. VBILacLac136773_0298.
Phylogenomic databases
eggNOG	COG1388.
HOGENOM	HOG000033783.
OMA	KAWRANA.
ProtClustDB	CLSK2567551.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-363-MONOMER.
Family and domain databases
InterPro	IPR000423. Flag_FlgJ_1. IPR013338. Lysozyme_dom_subfam2. IPR002901. Mano_Glyc_endo_b_GlcNAc. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF01832. Glucosaminidase. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
PRINTS	PR01002. FLGFLGJ.
SMART	SM00257. LysM. 3 hits. SM00047. LYZ2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACMA_LACLA

Accession

Primary (citable) accession number: Q9CIT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program