Q9CIQ1 (AMPN_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminopeptidase N
EC=3.4.11.2

Alternative name(s):
Alanine aminopeptidase
Lysyl aminopeptidase
Short name=Lys-AP

Gene names

Name:	pepN
Ordered Locus Names:	LL0305
ORF Names:	L102360

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Aminopeptidase with broad substrate specificity to several peptides. It has more affinity for oligopeptides than for dipeptides. It plays an essential role in the metabolism, it may be involved in nitrogen supply or protein turnover By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity. Note: It may be secreted through an unknown mechanism By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 846	846	Aminopeptidase N		PRO_0000095073
Regions
	Region	252 – 256	5	Substrate binding By similarity
Sites
	Active site	289	1	Proton acceptor By similarity
	Metal binding	288	1	Zinc; catalytic By similarity
	Metal binding	292	1	Zinc; catalytic By similarity
	Metal binding	311	1	Zinc; catalytic By similarity
	Binding site	120	1	Substrate By similarity
	Site	375	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CIQ1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 25AB6453A6A2681D	FASTA	846	95,336
	10 20 30 40 50 60 MAVKRLIETF VPENYKIFLD IDRKTKKIKG QVAITGEAKD SVIAFHAKGL HFSKVRAFSV 70 80 90 100 110 120 DTNFIENEED EEIVVKIGET GRVTVSFEYE AELTDNMMGI YPSYYEVNGE KKMLIGTQFE 130 140 150 160 170 180 SHFARQAFPS IDEPEAKATF DLSVKFDEEE GDIIVSNMPE LLNINGIHVF ERTVKMSSYL 190 200 210 220 230 240 LAFVFGELQF KKGKTKSGVE VGAFATKDHS EAALDFPLDI AIRSIEFYED YYKTPYPLPH 250 260 270 280 290 300 SWHIALPDFS AGAMENWGCI TYREVCMLVD PENATIQSKQ YVATVIAHEL AHQWFGDLVT 310 320 330 340 350 360 MQWWDDLWLN ESFANNMEYV CMDALEPSWN VWESFSISEA NMALNRDATD GVQSVHVEVT 370 380 390 400 410 420 HPDEIGTLFD PAIVYAKGSR LMVMLRKWLG DEDFAAGLAL YFKRHQYGNT VGDNLWDALA 430 440 450 460 470 480 EVSGKDVAAF MHSWVNQPGY PVVTAEVIDD TLVLSQKQFF VGEGADKGRL WNVPLNTNWS 490 500 510 520 530 540 GLPDLLSSEK VEIPGFAALK AKNDGKALFL NDANMAHYII DYKGQLLTDL LSEVETLENV 550 560 570 580 590 600 TKFQILQDRK LLAKAGVISY ADVVNILPAF TNEESYLVNT GLSQLISELE LFVDEDSETE 610 620 630 640 650 660 KAFQSLVGKL FAKNYARLGW DKVAGESAGD ESLRGIVLSK TLYAENADAK AKASQIFAAH 670 680 690 700 710 720 KENLAGIPAD IRPIVLNNEI KTTNSAELVK TYRETYVKTS LQEFKRELEG AVALIKDEKV 730 740 750 760 770 780 IAELLESFKN ADIVKPQDIA FSWFYLLRND FSQDAAWAWE KANWAFLEEK LGGDMSYDKF 790 800 810 820 830 840 VIYPGNTFKT ADKLAEYKAF FEPKLENQGL KRSIEMAIKQ ITARVALIDS QKADVDKSIK EISEKL

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04403.1.
PIR	A86663.
RefSeq	NP_266461.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CIQ1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113915.
GenomeReviews	Gene locus LL0305 in contig AE005176_GR.
KEGG	lla:L102360.
NMPDR	fig|272623.1.peg.313.
PATRIC	22292808. VBILacLac136773_0335.
Phylogenomic databases
HOGENOM	HBG515894.
OMA	LIGTQFE.
ProtClustDB	CLSK876725.
Enzyme and pathway databases
BioCyc	LLAC272623:L102360-MONOMER.
Family and domain databases
InterPro	IPR001930. Peptidase_M1. IPR014782. Peptidase_M1_N. [Graphical view]
KO	K01256.
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_LACLA

Accession

Primary (citable) accession number: Q9CIQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program