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Q9CIL5 (DDL_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LL0341
ORF Names:L140690
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177832

Regions

Domain132 – 335204ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2891Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 2 By similarity
Metal binding3041Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CIL5 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: 8E86996E1D2A8197

FASTA34938,693
        10         20         30         40         50         60 
MSKETLILLY GGRSAEREVS VLSAESVMRA VNYSRFVVKT YFITKGGEFI KTQEFTDKPA 

        70         80         90        100        110        120 
EEEKLLTNDL AESYPKISPA AIYEKAAVVF PVLHGPMGED GSIQGFLEIL RLAYVGPNIL 

       130        140        150        160        170        180 
SASSTMDKLL AKHVFEAVGV PQVPYVAAFA DENQGEIAQE VVEKLEFPVF VKPANMGSSV 

       190        200        210        220        230        240 
GISKVDDLAD LQPALSEAYK YDNRVVIEQG VDAREIECAV LGNNSDVSAT LPGEVVKDVE 

       250        260        270        280        290        300 
FYDYNSKYID NKIQMDIPAK VPADLAQKMQ EYAIKAYKAV NGTGLSRCDF FVTKDGNVYL 

       310        320        330        340 
NEINAIPGFT QWSMYPLLWE NMGLSYSDLI EKLVDLAKET FETRENHLL 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005176 Genomic DNA. Translation: AAK04439.1.
PIRE86667.
RefSeqNP_266497.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CIL5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L140690.

Proteomic databases

PRIDEQ9CIL5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK04439; AAK04439; L140690.
GeneID1113952.
KEGGlla:L140690.
PATRIC22292886. VBILacLac136773_0374.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAKLAFQYD.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-369-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LACLA
AccessionPrimary (citable) accession number: Q9CIL5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways