Q9CIL5 (DDL_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Ordered Locus Names:	LL0341
ORF Names:	L140690

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity HAMAP MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000177832
Regions
	Domain	132 – 335	204	ATP-grasp
	Nucleotide binding	162 – 217	56	ATP By similarity
Sites
	Metal binding	289	1	Magnesium or manganese 1 By similarity
	Metal binding	302	1	Magnesium or manganese 1 By similarity
	Metal binding	302	1	Magnesium or manganese 2 By similarity
	Metal binding	304	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CIL5 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 8E86996E1D2A8197	FASTA	349	38,693
	10 20 30 40 50 60 MSKETLILLY GGRSAEREVS VLSAESVMRA VNYSRFVVKT YFITKGGEFI KTQEFTDKPA 70 80 90 100 110 120 EEEKLLTNDL AESYPKISPA AIYEKAAVVF PVLHGPMGED GSIQGFLEIL RLAYVGPNIL 130 140 150 160 170 180 SASSTMDKLL AKHVFEAVGV PQVPYVAAFA DENQGEIAQE VVEKLEFPVF VKPANMGSSV 190 200 210 220 230 240 GISKVDDLAD LQPALSEAYK YDNRVVIEQG VDAREIECAV LGNNSDVSAT LPGEVVKDVE 250 260 270 280 290 300 FYDYNSKYID NKIQMDIPAK VPADLAQKMQ EYAIKAYKAV NGTGLSRCDF FVTKDGNVYL 310 320 330 340 NEINAIPGFT QWSMYPLLWE NMGLSYSDLI EKLVDLAKET FETRENHLL

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04439.1.
PIR	E86667.
RefSeq	NP_266497.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CIL5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113952.
GenomeReviews	Gene locus LL0341 in contig AE005176_GR.
KEGG	lla:L140690.
NMPDR	fig|272623.1.peg.349.
PATRIC	22292886. VBILacLac136773_0374.
Phylogenomic databases
HOGENOM	HBG427092.
OMA	LLHGPFG.
ProtClustDB	PRK01966.
Enzyme and pathway databases
BioCyc	LLAC272623:L140690-MONOMER.
Family and domain databases
HAMAP	MF_00047. Dala_Dala_lig. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01921.
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_LACLA

Accession

Primary (citable) accession number: Q9CIL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program