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Protein

Trehalose 6-phosphate phosphorylase

Gene

trePP

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of trehalose 6-phosphate into glucose 1-phosphate and glucose 6-phosphate.

Catalytic activityi

Alpha,alpha-trehalose 6-phosphate + phosphate = glucose 6-phosphate + beta-D-glucose 1-phosphate.

pH dependencei

Optimum pH is 6.3.

Temperature dependencei

Optimum temperature is 35 degrees Celsius.

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. trehalose 6-phosphate phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-458-MONOMER.
MetaCyc:MONOMER-5861.
BRENDAi2.4.1.216. 2903.
SABIO-RKQ9CID5.

Protein family/group databases

CAZyiGH65. Glycoside Hydrolase Family 65.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose 6-phosphate phosphorylase (EC:2.4.1.216)
Short name:
TrePP
Gene namesi
Name:trePP
Ordered Locus Names:LL0428
ORF Names:L39593
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 769768Trehalose 6-phosphate phosphorylasePRO_0000108014Add
BLAST

Proteomic databases

PRIDEiQ9CID5.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi272623.L39593.

Structurei

3D structure databases

ProteinModelPortaliQ9CID5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 65 family.Curated

Phylogenomic databases

eggNOGiCOG1554.
HOGENOMiHOG000237472.
KOiK03731.
OMAiNIYRMDR.
OrthoDBiEOG62C99Q.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.70.98.40. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005194. Glyco_hydro_65_C.
IPR005195. Glyco_hydro_65_M.
IPR005196. Glyco_hydro_65_N.
IPR017045. Malt_Pase/Glycosyl_Hdrlase.
[Graphical view]
PfamiPF03633. Glyco_hydro_65C. 1 hit.
PF03632. Glyco_hydro_65m. 1 hit.
PF03636. Glyco_hydro_65N. 1 hit.
[Graphical view]
PIRSFiPIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CID5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKDWIIQY DKKEVGKRSY GQESLMSLGN GYLGLRGAPL WSTCSDNHYP
60 70 80 90 100
GLYVAGVFNR TSTEVAGHDV INEDMVNWPN PQLIKVYIDG ELVDFEASVE
110 120 130 140 150
KQATIDFKNA LQIERYQVKL AKGNLTLVTT KFVDPINFHD FGFVGEIIAD
160 170 180 190 200
FSCKLRIETF TDGSVLNQNV ERYRAFDSKE FEVTKISKGL LVAKTRTSEI
210 220 230 240 250
ELAIASKSFL NGLAFPKIDS ENDEILAEAI EIDLQKNQEV QFDKTIVIAS
260 270 280 290 300
SYESKNPVEF VLTELSATSV SKIQENNTNY WEKVWSDADI VIESDHEDLQ
310 320 330 340 350
RMVRMNIFHI RQAAQHGANQ FLDASVGSRG LTGEGYRGHI FWDEIFVLPY
360 370 380 390 400
YAANEPETAR DLLLYRINRL TAAQENAKVD GEKGAMFPWQ SGLIGDEQSQ
410 420 430 440 450
FVHLNTVNNE WEPDNSRRQR HVSLAIVYNL WIYSQLTEDE SILTDGGLDL
460 470 480 490 500
IIETTKFWLN KAELGDDGRY HIDGVMGPDE YHEAYPGQEG GICDNAYTNL
510 520 530 540 550
MLTWQLNWLT ELSEKGFEIP KELLEKAQKV RKKLYLDIDE NGVIAQYAKY
560 570 580 590 600
FELKEVDFAA YEAKYGDIHR IDRLMKAEGI SPDEYQVAKQ ADTLMLIYNL
610 620 630 640 650
GQEHVTKLVK QLAYELPENW LKVNRDYYLA RTVHGSTTSR PVFAGIDVKL
660 670 680 690 700
GDFDEALDFL ITAIGSDYYD IQGGTTAEGV HIGVMGETLE VIQNEFAGLS
710 720 730 740 750
LREGQFAIAP YLPKSWTKLK FNQIFRGTKV EILIENGQLL LTASADLLTK
760
VYDDEVQLKA GVQTKFDLK
Length:769
Mass (Da):87,267
Last modified:January 23, 2007 - v3
Checksum:i562D096E3A6CBB72
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti703 – 7031E → D in strain: ATCC 19435.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18267 Genomic DNA. Translation: CAA77100.1.
AE005176 Genomic DNA. Translation: AAK04526.1.
PIRiD86678.
RefSeqiNP_266584.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK04526; AAK04526; L39593.
GeneIDi1114040.
KEGGilla:L39593.
PATRICi22293072. VBILacLac136773_0466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18267 Genomic DNA. Translation: CAA77100.1.
AE005176 Genomic DNA. Translation: AAK04526.1.
PIRiD86678.
RefSeqiNP_266584.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CID5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L39593.

Protein family/group databases

CAZyiGH65. Glycoside Hydrolase Family 65.

Proteomic databases

PRIDEiQ9CID5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK04526; AAK04526; L39593.
GeneIDi1114040.
KEGGilla:L39593.
PATRICi22293072. VBILacLac136773_0466.

Phylogenomic databases

eggNOGiCOG1554.
HOGENOMiHOG000237472.
KOiK03731.
OMAiNIYRMDR.
OrthoDBiEOG62C99Q.

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-458-MONOMER.
MetaCyc:MONOMER-5861.
BRENDAi2.4.1.216. 2903.
SABIO-RKQ9CID5.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.70.98.40. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005194. Glyco_hydro_65_C.
IPR005195. Glyco_hydro_65_M.
IPR005196. Glyco_hydro_65_N.
IPR017045. Malt_Pase/Glycosyl_Hdrlase.
[Graphical view]
PfamiPF03633. Glyco_hydro_65C. 1 hit.
PF03632. Glyco_hydro_65m. 1 hit.
PF03636. Glyco_hydro_65N. 1 hit.
[Graphical view]
PIRSFiPIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis."
    Andersson U., Levander F., Raedstroem P.
    J. Biol. Chem. 276:42707-42713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION.
    Strain: ATCC 19435 / DSM 20481 / NCDO 604 / NCIB 6681 / NCTC 6681.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiTREPP_LACLA
AccessioniPrimary (citable) accession number: Q9CID5
Secondary accession number(s): Q9ZAG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.