Q9CID5 (TREPP_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalose 6-phosphate phosphorylase
Short name=TrePP
EC=2.4.1.216

Gene names

Name:	trePP
Ordered Locus Names:	LL0428
ORF Names:	L39593

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of trehalose 6-phosphate into glucose 1-phosphate and glucose 6-phosphate.
Catalytic activity	Alpha,alpha-trehalose 6-phosphate + phosphate = glucose 6-phosphate + beta-D-glucose 1-phosphate.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 65 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.3. Temperature dependence: Optimum temperature is 35 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro trehalose 6-phosphate phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 769	768	Trehalose 6-phosphate phosphorylase		PRO_0000108014
Natural variations
	Natural variant	703	1	E → D in strain: ATCC 19435.

Sequences

	Sequence		Length	Mass (Da)
	Q9CID5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 562D096E3A6CBB72	FASTA	769	87,267
	10 20 30 40 50 60 MTEKDWIIQY DKKEVGKRSY GQESLMSLGN GYLGLRGAPL WSTCSDNHYP GLYVAGVFNR 70 80 90 100 110 120 TSTEVAGHDV INEDMVNWPN PQLIKVYIDG ELVDFEASVE KQATIDFKNA LQIERYQVKL 130 140 150 160 170 180 AKGNLTLVTT KFVDPINFHD FGFVGEIIAD FSCKLRIETF TDGSVLNQNV ERYRAFDSKE 190 200 210 220 230 240 FEVTKISKGL LVAKTRTSEI ELAIASKSFL NGLAFPKIDS ENDEILAEAI EIDLQKNQEV 250 260 270 280 290 300 QFDKTIVIAS SYESKNPVEF VLTELSATSV SKIQENNTNY WEKVWSDADI VIESDHEDLQ 310 320 330 340 350 360 RMVRMNIFHI RQAAQHGANQ FLDASVGSRG LTGEGYRGHI FWDEIFVLPY YAANEPETAR 370 380 390 400 410 420 DLLLYRINRL TAAQENAKVD GEKGAMFPWQ SGLIGDEQSQ FVHLNTVNNE WEPDNSRRQR 430 440 450 460 470 480 HVSLAIVYNL WIYSQLTEDE SILTDGGLDL IIETTKFWLN KAELGDDGRY HIDGVMGPDE 490 500 510 520 530 540 YHEAYPGQEG GICDNAYTNL MLTWQLNWLT ELSEKGFEIP KELLEKAQKV RKKLYLDIDE 550 560 570 580 590 600 NGVIAQYAKY FELKEVDFAA YEAKYGDIHR IDRLMKAEGI SPDEYQVAKQ ADTLMLIYNL 610 620 630 640 650 660 GQEHVTKLVK QLAYELPENW LKVNRDYYLA RTVHGSTTSR PVFAGIDVKL GDFDEALDFL 670 680 690 700 710 720 ITAIGSDYYD IQGGTTAEGV HIGVMGETLE VIQNEFAGLS LREGQFAIAP YLPKSWTKLK 730 740 750 760 FNQIFRGTKV EILIENGQLL LTASADLLTK VYDDEVQLKA GVQTKFDLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis." Andersson U., Levander F., Raedstroem P. J. Biol. Chem. 276:42707-42713(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION. Strain: ATCC 19435 / DSM 20481 / NCDO 604 / NCIB 6681 / NCTC 6681.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18267 Genomic DNA. Translation: CAA77100.1. AE005176 Genomic DNA. Translation: AAK04526.1.
PIR	D86678.
RefSeq	NP_266584.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CID5.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L39593.
Protein family/group databases
CAZy	GH65. Glycoside Hydrolase Family 65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK04526; AAK04526; L39593.
GeneID	1114040.
KEGG	lla:L39593.
PATRIC	22293072. VBILacLac136773_0466.
Phylogenomic databases
eggNOG	COG1554.
HOGENOM	HOG000237472.
KO	K03731.
OMA	YRAFDSK.
ProtClustDB	CLSK697226.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-458-MONOMER. MetaCyc:MONOMER-5861.
SABIO-RK	Q9CID5.
Family and domain databases
Gene3D	1.50.10.10. 1 hit. 2.70.98.40. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR011013. Gal_mutarotase_SF_dom. IPR005194. Glyco_hydro_65_C. IPR005195. Glyco_hydro_65_M. IPR005196. Glyco_hydro_65_N. IPR017045. Malt_Pase/Glycosyl_Hdrlase. [Graphical view]
Pfam	PF03633. Glyco_hydro_65C. 1 hit. PF03632. Glyco_hydro_65m. 1 hit. PF03636. Glyco_hydro_65N. 1 hit. [Graphical view]
PIRSF	PIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TREPP_LACLA

Accession

Primary (citable) accession number: Q9CID5
Secondary accession number(s): Q9ZAG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program