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Q9CID5 (TREPP_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trehalose 6-phosphate phosphorylase

Short name=TrePP
EC=2.4.1.216
Gene names
Name:trePP
Ordered Locus Names:LL0428
ORF Names:L39593
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of trehalose 6-phosphate into glucose 1-phosphate and glucose 6-phosphate.

Catalytic activity

Alpha,alpha-trehalose 6-phosphate + phosphate = glucose 6-phosphate + beta-D-glucose 1-phosphate.

Subunit structure

Monomer.

Sequence similarities

Belongs to the glycosyl hydrolase 65 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 6.3.

Temperature dependence:

Optimum temperature is 35 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 769768Trehalose 6-phosphate phosphorylase
PRO_0000108014

Natural variations

Natural variant7031E → D in strain: ATCC 19435.

Sequences

Sequence LengthMass (Da)Tools
Q9CID5 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 562D096E3A6CBB72

FASTA76987,267
        10         20         30         40         50         60 
MTEKDWIIQY DKKEVGKRSY GQESLMSLGN GYLGLRGAPL WSTCSDNHYP GLYVAGVFNR 

        70         80         90        100        110        120 
TSTEVAGHDV INEDMVNWPN PQLIKVYIDG ELVDFEASVE KQATIDFKNA LQIERYQVKL 

       130        140        150        160        170        180 
AKGNLTLVTT KFVDPINFHD FGFVGEIIAD FSCKLRIETF TDGSVLNQNV ERYRAFDSKE 

       190        200        210        220        230        240 
FEVTKISKGL LVAKTRTSEI ELAIASKSFL NGLAFPKIDS ENDEILAEAI EIDLQKNQEV 

       250        260        270        280        290        300 
QFDKTIVIAS SYESKNPVEF VLTELSATSV SKIQENNTNY WEKVWSDADI VIESDHEDLQ 

       310        320        330        340        350        360 
RMVRMNIFHI RQAAQHGANQ FLDASVGSRG LTGEGYRGHI FWDEIFVLPY YAANEPETAR 

       370        380        390        400        410        420 
DLLLYRINRL TAAQENAKVD GEKGAMFPWQ SGLIGDEQSQ FVHLNTVNNE WEPDNSRRQR 

       430        440        450        460        470        480 
HVSLAIVYNL WIYSQLTEDE SILTDGGLDL IIETTKFWLN KAELGDDGRY HIDGVMGPDE 

       490        500        510        520        530        540 
YHEAYPGQEG GICDNAYTNL MLTWQLNWLT ELSEKGFEIP KELLEKAQKV RKKLYLDIDE 

       550        560        570        580        590        600 
NGVIAQYAKY FELKEVDFAA YEAKYGDIHR IDRLMKAEGI SPDEYQVAKQ ADTLMLIYNL 

       610        620        630        640        650        660 
GQEHVTKLVK QLAYELPENW LKVNRDYYLA RTVHGSTTSR PVFAGIDVKL GDFDEALDFL 

       670        680        690        700        710        720 
ITAIGSDYYD IQGGTTAEGV HIGVMGETLE VIQNEFAGLS LREGQFAIAP YLPKSWTKLK 

       730        740        750        760 
FNQIFRGTKV EILIENGQLL LTASADLLTK VYDDEVQLKA GVQTKFDLK 

« Hide

References

« Hide 'large scale' references
[1]"Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis."
Andersson U., Levander F., Raedstroem P.
J. Biol. Chem. 276:42707-42713(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION.
Strain: ATCC 19435 / DSM 20481 / NCDO 604 / NCIB 6681 / NCTC 6681.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18267 Genomic DNA. Translation: CAA77100.1.
AE005176 Genomic DNA. Translation: AAK04526.1.
PIRD86678.
RefSeqNP_266584.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CID5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L39593.

Protein family/group databases

CAZyGH65. Glycoside Hydrolase Family 65.

Proteomic databases

PRIDEQ9CID5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK04526; AAK04526; L39593.
GeneID1114040.
KEGGlla:L39593.
PATRIC22293072. VBILacLac136773_0466.

Phylogenomic databases

eggNOGCOG1554.
HOGENOMHOG000237472.
KOK03731.
OMARTVDYYL.
OrthoDBEOG62C99Q.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-458-MONOMER.
MetaCyc:MONOMER-5861.
SABIO-RKQ9CID5.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.70.98.40. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005194. Glyco_hydro_65_C.
IPR005195. Glyco_hydro_65_M.
IPR005196. Glyco_hydro_65_N.
IPR017045. Malt_Pase/Glycosyl_Hdrlase.
[Graphical view]
PfamPF03633. Glyco_hydro_65C. 1 hit.
PF03632. Glyco_hydro_65m. 1 hit.
PF03636. Glyco_hydro_65N. 1 hit.
[Graphical view]
PIRSFPIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
SUPFAMSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTREPP_LACLA
AccessionPrimary (citable) accession number: Q9CID5
Secondary accession number(s): Q9ZAG0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries