Q9CI70 (DPO3A_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7

Gene names

Name:	dnaE
Ordered Locus Names:	LL0496
ORF Names:	L0307

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	1060 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1060	1060	DNA polymerase III subunit alpha		PRO_0000103325

Sequences

	Sequence		Length	Mass (Da)
	Q9CI70 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 44B03F0937A8D3E6	FASTA	1,060	121,572
	10 20 30 40 50 60 MFAPLNTKTE YSFLDSVVKV DDYLETAHRL GYQTVGICDV GNLHAAFRFV RKAQKFNLQP 70 80 90 100 110 120 IISIELNFEW RGLPIAFSFI AKDTEGYKNL LRISTLHNYG RRQFSDIQNH LSGIALIIPE 130 140 150 160 170 180 TYGSLSELTE LSSVADEAFI GIDQLTDKGA KFNLPTLPFP AIRYLNFADN EILAILHAVR 190 200 210 220 230 240 DGVSFDESLT ISSNELLQRP EAYETYFLKY FPQALKNLSA LTAKIAYQFD EKLELPRFDK 250 260 270 280 290 300 KRQAVEHLRE EATLGLSARL EEKNLLTEVS SVNLQDLSVY QERLEKELSV IHQMGFDDYF 310 320 330 340 350 360 LIVADLLRYA REQDIYCGMG RGSAAGSLVA YVLGITQVDP VQHNLFFERF LNPERVAMPD 370 380 390 400 410 420 IDIDMPDDRR AELLAYMKNR YGSDHVAQIV TFSTLGKRQA LRDVGKAFGM TEAELSNLTR 430 440 450 460 470 480 MLVRRFGTLA DEYENNQRFK AEVLKNGKLK RIYEVARRIE GMPRQTSTHA SGVVLSEESL 490 500 510 520 530 540 VNYVSLKPSE DLALTQYEAP DVEAIGLLKI DFLGLRNLTI ISRLRDLVKR RQKIDIDPLK 550 560 570 580 590 600 INLEDEDTLA LFRAGNTMGI FQFENPQMRR FLRNLAPSKF DDIVDATSIF RPGPSQFIPQ 610 620 630 640 650 660 FVARRHGKEI VPTVDDSISE ILKPTYGIMI YQEQIMQVAQ AFAGFSLGKA DLLRRAISKK 670 680 690 700 710 720 KGSELEKLRE DFLDSASHNG HSKEKAEEIY DLIERFANYG FNRSHGFAYG ALAFQIAYFK 730 740 750 760 770 780 AHFPDEFYEI QLRDRKREVM ILDALENGFE IEKPSINLMK IGDFVKNKKI RLGLAHVQGI 790 800 810 820 830 840 SRDLAKWIVE NQPYKDLADF VEKLPNNFHK KENILPLIQI GAFDYADSNR GKLAYNLADH 850 860 870 880 890 900 ANLLNYYSDD IFMASSGGGF AYHEAEDYSE TEKYDFEKNL LGIGVTPHPL QNLARRFEGN 910 920 930 940 950 960 FTPLAQLVKN RRMTILVEIN YIRTHRTKTG QTMAFLTVSD SRENFDVTLF PEIYRQFSGE 970 980 990 1000 1010 1020 LEQGKFYLIS GKVTERNDEL QLVTDRISPA LETDRKLWLN LRDDKYNQKI SQILREFPGS 1030 1040 1050 1060 HQVILHYSNL KKTIQTKIYV EESEILQKRL EGYVIAGIYK

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04594.1.
PIR	H86686.
RefSeq	NP_266652.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CI70.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114115.
GenomeReviews	Gene locus LL0496 in contig AE005176_GR.
KEGG	lla:L0307.
NMPDR	fig|272623.1.peg.511.
PATRIC	22293222. VBILacLac136773_0539.
Phylogenomic databases
HOGENOM	HBG734490.
OMA	IHQMGFD.
ProtClustDB	PRK07279.
Enzyme and pathway databases
BioCyc	LLAC272623:L0307-MONOMER.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K02337.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3A_LACLA

Accession

Primary (citable) accession number: Q9CI70

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program