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Q9CHW7 (GLYA_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:LL0601
ORF Names:L0082
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_0000113591

Regions

Region125 – 1273Substrate binding By similarity

Sites

Binding site351Pyridoxal phosphate By similarity
Binding site551Pyridoxal phosphate By similarity
Binding site571Substrate By similarity
Binding site641Substrate binding By similarity
Binding site651Pyridoxal phosphate By similarity
Binding site991Pyridoxal phosphate By similarity
Binding site1211Substrate By similarity
Binding site1761Pyridoxal phosphate By similarity
Binding site2041Pyridoxal phosphate By similarity
Binding site2291Pyridoxal phosphate By similarity
Binding site2361Pyridoxal phosphate By similarity
Binding site2621Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3631Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2301N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CHW7 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: 593559F38C4718C0

FASTA41544,790
        10         20         30         40         50         60 
MIFDKEDFES FDPELWAAIH AEEIRQQQNI ELIASENIVS KAVMAAQGSV LTNKYAEGYP 

        70         80         90        100        110        120 
GKRYYGGTEA VDVVENLAIE RAKELFGAKF ANVQPHSGSQ ANAAAYMALI QPGDTVLGMD 

       130        140        150        160        170        180 
LNAGGHLTHG ASVNFSGKTY HFVPYGVNSE TELLDYDEIL KIAKQVQPKL IVAGASAYSR 

       190        200        210        220        230        240 
LIDFAKFREI ADSVGAKLMV DMAHIAGLVA TGAHPNPLPY ADVVTTTTHK TLRGPRGGMI 

       250        260        270        280        290        300 
LTNDEALAKK INSAIFPGTQ GGPLEHVIAA KAVAFKEALD PEFTTYIEQV IKNTQAMAEE 

       310        320        330        340        350        360 
FAKVEGLRLI AGGSDNHLLN LKVLDLGING KEAQDLLDSV HITLNKEAIP DETLSPFKTS 

       370        380        390        400        410 
GVRIGAAAIT SRGFKEVEAK KVAQLVSEAL VNHDNQEKLA EVRKAALELT RQFPL

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005176 Genomic DNA. Translation: AAK04699.1.
PIRA86700.
RefSeqNP_266757.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CHW7.
SMRQ9CHW7. Positions 12-407.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1114221.
GenomeReviewsGene locus LL0601 in contig AE005176_GR.
KEGGlla:L0082.
NMPDRfig|272623.1.peg.616.
PATRIC22293464. VBILacLac136773_0641.

Phylogenomic databases

HOGENOMHBG301263.
OMAFSASYEM.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycLLAC272623:L0082-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_LACLA
AccessionPrimary (citable) accession number: Q9CHW7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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