Q9CHK0 (MEND_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Short name=SEPHCHC synthase
EC=2.2.1.9

Alternative name(s):
Menaquinone biosynthesis protein MenD

Gene names

Name:	menD
Ordered Locus Names:	LL0731
ORF Names:	L0169

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP MF_01659
Catalytic activity	Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP MF_01659
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01659 Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP MF_01659
Subunit structure	Homodimer By similarity. HAMAP MF_01659
Sequence similarities	Belongs to the TPP enzyme family. MenD subfamily.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	Magnesium Manganese Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP MF_01659		PRO_0000341763

Sequences

	Sequence		Length	Mass (Da)
	Q9CHK0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F6B9686536DB332C	FASTA	560	62,524
	10 20 30 40 50 60 MTNEYLAPFV DELFNLGVRE AVFSPGSRST ALAMLFEEYK KYDTYVNIDE RSAAFFALGI 70 80 90 100 110 120 AKAKKRPVVL VCTSGSAAAH HFPAVLEAKM SRVPLIILTA DRPAELQFVG APQTVDQTRF 130 140 150 160 170 180 FGNFVNHFEN LEAPHIQKQS QTENFWTYPR KVAQRAVLSA ISPLSGPVQI NVPLRDLLVP 190 200 210 220 230 240 ELKSENYEKG RSKHAFKFYE GQAQVILPFD EALLSGKTLI LAGANFDKDY SEALLKLAEQ 250 260 270 280 290 300 LKAPILADPL SNLRNHNHPL VIDSYDAFLA NNDLKTQLKA DAILLFGQMP VSKRLQQFVA 310 320 330 340 350 360 FNNEAEFIQV DPALDYRNPS LTTTTMVQSD VLPFASSIKK VNSDSSYLEK WQNAQEKMRQ 370 380 390 400 410 420 LLEKVTYEES PFEGRYVQEL QKHLEALDAQ LLVSNSMEIR DIDYWWKKAD AKVRILGNRG 430 440 450 460 470 480 VNGIDGTEST ALGIATTGKP TVLLTGDLSM LHDLNGLIVG KTHELNLTIV LFNNDGGGIF 490 500 510 520 530 540 HHLAQKGVPN FDYLFSTPHG LNFAGLAELT GLDYHLVSGY ADFGQQFEAS LHQSGIHLLE 550 560 IKTDKDLSLA LHQKYTTYEN

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04829.1.
PIR	C86716.
RefSeq	NP_266887.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CHK0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114357.
GenomeReviews	Gene locus LL0731 in contig AE005176_GR.
KEGG	lla:L0169.
NMPDR	fig|272623.1.peg.750.
PATRIC	22293756. VBILacLac136773_0785.
Phylogenomic databases
HOGENOM	HBG634405.
OMA	ICTSGTA.
ProtClustDB	CLSK697379.
Enzyme and pathway databases
BioCyc	LLAC272623:L0169-MONOMER.
Family and domain databases
HAMAP	MF_01659. MenD. [Tree]
InterPro	IPR004433. MenaQ_synth_MenD. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K02551.
PANTHER	PTHR18968:SF3. PTHR18968:SF3. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PIRSF	PIRSF004983. MenD. 1 hit.
TIGRFAMs	TIGR00173. MenD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MEND_LACLA

Accession

Primary (citable) accession number: Q9CHK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program