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Q9CHI2

- TRMB_LACLA

UniProt

Q9CHI2 - TRMB_LACLA

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
    Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
    Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
    Active sitei118 – 1181By similarity
    Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
    Binding sitei122 – 1221SubstrateUniRule annotation
    Binding sitei154 – 1541SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-815-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:LL0749
    ORF Names:L156302
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_0000171338Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272623.L156302.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CHI2.
    SMRiQ9CHI2. Positions 6-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1296Interaction with RNAUniRule annotation
    Regioni193 – 1964Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CHI2-1 [UniParc]FASTAAdd to Basket

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    MRVRNRKGAG EMLAENAHIV VENPADFKGR WSERFGNDHP IHIEVGCGKG    50
    AFITGMAALH PEINYIAIDM QLSVLSYALD KAIEADLPNV QMMLVDGAAL 100
    SEYFADGEID QVYLNFSDPW PKGRHEKRRL TYKSFLATYE KILRPEGEIH 150
    FKTDNRGLFE YSLVSLANYG MELKKVWLDL HQDEEFAPQN VMTEYEQKFS 200
    QKGQVIYRLE AKFLPKK 217
    Length:217
    Mass (Da):24,947
    Last modified:June 1, 2001 - v1
    Checksum:i11F0A543DD4DE0AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005176 Genomic DNA. Translation: AAK04847.1.
    PIRiE86718.
    RefSeqiNP_266905.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK04847; AAK04847; L156302.
    GeneIDi1114375.
    KEGGilla:L156302.
    PATRICi22293792. VBILacLac136773_0803.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005176 Genomic DNA. Translation: AAK04847.1 .
    PIRi E86718.
    RefSeqi NP_266905.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q9CHI2.
    SMRi Q9CHI2. Positions 6-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L156302.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK04847 ; AAK04847 ; L156302 .
    GeneIDi 1114375.
    KEGGi lla:L156302.
    PATRICi 22293792. VBILacLac136773_0803.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci LLAC272623:GHSH-815-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiTRMB_LACLA
    AccessioniPrimary (citable) accession number: Q9CHI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3