Reviewed, UniProtKB/Swiss-Prot Q9CHB8 (KPRS1_LACLA)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
      Short name=RPPK 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1
      Short name=P-Rib-PP synthetase 1
      Short name=PRPP synthetase 1

Gene names

Name:	prs1
Synonyms:	prsA
Ordered Locus Names:	LL0814
ORF Names:	L25614

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583		PRO_0000141146
Regions
	Region	216 – 229	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CHB8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C7AC243CF13FDF28	FASTA	324	35,545
	10 20 30 40 50 60 MSYSNLKLFS LSSNHELAQK VAKEIGIELG KVSVGAHSDG ETVVHIDESV RGDHVFILQS 70 80 90 100 110 120 TSDPVNDNLM ELLIMMDALR RASAASINIV LPYYGYARQD RKARAREPIT SKLVANMLQI 130 140 150 160 170 180 AGADRLITFD LHAPQIQGFF NIPVDHLMGS PLIAEYFRRQ LVSAGDDIVV VSPDHGGVGR 190 200 210 220 230 240 ARKLANFLKA PLSIIDKRRP RANVAEIMNI IGDVQGKKCI LIDDMIDTAG TITLAANALK 250 260 270 280 290 300 ELGATEVYAS CTHAVLSGPA IERINNSAIT KLVVLDTIEM PEERQSEKIV QLSIAHLLAD 310 320 AIIRIHERRP LSPLFELHLP SEQI

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005176 Genomic DNA. Translation: AAK04912.1.
PIR	F86726.
RefSeq	NP_266970.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1114441.
GenomeReviews	Gene locus LL0814 in contig AE005176_GR.
KEGG	lla:L25614.
NMPDR	fig|272623.1.peg.834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CHB8.
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	LLAC272623:L25614-MON.
BRENDA	2.7.6.1. 278870.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_LACLA

Accession

Primary (citable) accession number: Q9CHB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)