Q9CH07 (FTHS_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	LL0935
ORF Names:	L159505

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199355
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CH07 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BEF24F56C1B259A1	FASTA	555	59,443
	10 20 30 40 50 60 MKTDIEIAQA ADIQPITKIA EKIGLSFDDI ELYGKYKAKI PLEVLDKFDQ QSEGKLVLVT 70 80 90 100 110 120 SINPTPAGEG KSTVTVGLAD AFARQGKNVM VALREPSLGP VMGIKGGAAG GGFAQVLPME 130 140 150 160 170 180 DINLHFTGDI HAITTANNAI SAFLDNSLHQ GNPLNIDPRR IIWKRVVDLN DRALRHVTVG 190 200 210 220 230 240 LGGPLNGVPR EDGFDITVVS EIMAVLCLAT SISDLKERLG KIVLAQSYDR KPVTLGDLGV 250 260 270 280 290 300 QGAIAMLLKD ALKPNLVQTI EGTPALIHGG PFANIAHGCN SVLATKTALK LSDIVITEAG 310 320 330 340 350 360 FGADLGGEKF LDIKTRQLGK QPDAVVIVAT LRALKMHGGL DKKELTKENV EAVKKGFANL 370 380 390 400 410 420 ERHIKNMQSY GLPVIVAINE FASDTKSEIS ALKDLTEALG VPVSLTQVFA KGGEGGLDLA 430 440 450 460 470 480 EKLSGMLQEK SDFSYLYDLK EPLSAKIDKV VTEIYGGSKV NYSPKAKRQM REIEENGWND 490 500 510 520 530 540 LPVCMAKTQY SFSDQPNLLA APEGFEVTVR ELLPKIGAGF IVALLGDVMT MPGLPKNPAS 550 LKMDVTDDGK ISGLF

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05033.1.
PIR	G86741.
RefSeq	NP_267091.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CH07.
SMR	Q9CH07. Positions 4-553.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L159505.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05033; AAK05033; L159505.
GeneID	1114565.
KEGG	lla:L159505.
PATRIC	22294186. VBILacLac136773_1000.
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-1005-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_LACLA

Accession

Primary (citable) accession number: Q9CH07

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program