Q9CGJ4 (NADE_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	LL1102
ORF Names:	L0203

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152175
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CGJ4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CC672DC1649EE2D0	FASTA	274	30,281
	10 20 30 40 50 60 MTLQDEIIKE LGVKPVIDPK EEIRVSVDFL KDYLKKYPFI KSFVLGISGG QDSSLAGRLA 70 80 90 100 110 120 QIAIEEMRQE TADETYKFVA IRLPYGVQAD EEDAQRALAF IQPDVSLTVN IKAAVEGQVA 130 140 150 160 170 180 ALNEAGIEVS DFNKGNIKAR QRMITQYAVA GQYQGAVLGT DHAAENITGF FTKFGDGGAD 190 200 210 220 230 240 LLPLFRLNKR QGKALLAELG ADPAIYEKVP TADLEEGKPG LADEIALGVT YNDIDDYTEG 250 260 270 KVISEDAKAK IEAWWKKTQH KRHLPISVFD DFWK

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05200.1.
PIR	F86762.
RefSeq	NP_267258.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CGJ4.
SMR	Q9CGJ4. Positions 2-274.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114742.
GenomeReviews	Gene locus LL1102 in contig AE005176_GR.
KEGG	lla:L0203.
NMPDR	fig|272623.1.peg.1134.
PATRIC	22294546. VBILacLac136773_1180.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	LLAC272623:L0203-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_LACLA

Accession

Primary (citable) accession number: Q9CGJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program