Reviewed, UniProtKB/Swiss-Prot Q9CGG8 (LDH3_LACLA)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 3
      Short name=L-LDH 3
    EC=1.1.1.27

Gene names

Name:	ldhX
Ordered Locus Names:	LL1128
ORF Names:	L142159

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	L-lactate dehydrogenase 3 HAMAP MF_00488		PRO_0000168353
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	182	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	127	1	NAD or substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CGG8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 87CCB4EA290A15DD	FASTA	323	35,271
	10 20 30 40 50 60 MKINNKKVVI VGAGAVGSTY AHNLVVDDLA DEIAIINTNK SKASANSLDL LHALPYLNAA 70 80 90 100 110 120 PKNIYAADYS DVSDADIVVL SANAPSATFG KNPDRLQLLE NKVEMIRDIT RKTMDAGFDG 130 140 150 160 170 180 IFLVASNPVD VLAQVVAEVS GLPKHRVIGT GTLLETSRMR QIVAEKLQIN PKSIHGYVLA 190 200 210 220 230 240 EHGKSSFAAW SNVTVGAIPL TTWLKKYPNP EFPTFDEIDQ EIREVGLDIF MQKGNTSYGI 250 260 270 280 290 300 AASLARLTRA IFRNESVILP VSAYLTGEYG QFDLYTGSPA IIDRTGVRAV LELELTQEEQ 310 320 EKFKASTVLL KENFDSIKEK CTL

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005176 Genomic DNA. Translation: AAK05226.1.
PIR	H86765.
RefSeq	NP_267284.1.
3D structure databases
HSSP	HSSP built from PDB template 2LDB based on UniProtKB P00344.
ModBase	Search...
Genome annotation databases
GeneID	1114768.
GenomeReviews	Gene locus LL1128 in contig AE005176_GR.
KEGG	lla:L142159.
NMPDR	fig|272623.1.peg.1160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CGG8.
OMA	LENLASC.
Enzyme and pathway databases
BioCyc	LLAC272623:L142159-MON.
BRENDA	1.1.1.27. 278870.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH3_LACLA

Accession

Primary (citable) accession number: Q9CGG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)