Reviewed, UniProtKB/Swiss-Prot Q9CGF1 (GUAC_LACLA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LL1145
ORF Names:	L158186

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	GMP reductase HAMAP MF_01511		PRO_0000093761
Regions
	Nucleotide binding	207 – 230	24	NADP By similarity
Sites
	Active site	178	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CGF1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 187BDCC3564FDA17	FASTA	329	35,837
	10 20 30 40 50 60 MNNLNSVFDY EDIQLIPNKC VINSRLEADT SVKLGNFTFK LPVVPANMQT IIDDKIAEML 70 80 90 100 110 120 AKEGYFYIMH RFEAENRAAF IKKMHQQGLI ASISVGVKAD EHAFIREISA DALIPEFITI 130 140 150 160 170 180 DIAHGHADSV IKTIQLIKRL MPQTFVIAGN VGTPEAVREL ENAGADATKV GIGPGKVCIT 190 200 210 220 230 240 KVKTGFGTGG WQLAAVKWCA KAASKPVIAD GGIRTHGDIA KSIRMGATMV MVGSLFAAHE 250 260 270 280 290 300 ESPGQTVERD GQLFKEYFGS ASEYQKGEHK NVEGKKILLP HKGSLKDTLK EMEEDLQSSI 310 320 SYAGGRDLSA LTKVDYVVVK NSIWNGDAI

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005176 Genomic DNA. Translation: AAK05243.1.
PIR	A86768.
RefSeq	NP_267301.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
SMR	Q9CGF1. Positions 4-324.
ModBase	Search...
Genome annotation databases
GeneID	1114786.
GenomeReviews	Gene locus LL1145 in contig AE005176_GR.
KEGG	lla:L158186.
NMPDR	fig|272623.1.peg.1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CGF1.
OMA	NSRSECD.
Enzyme and pathway databases
BioCyc	LLAC272623:L158186-MON.
BRENDA	1.7.1.7. 278870.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACLA

Accession

Primary (citable) accession number: Q9CGF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)