Q9CGD7 (KHSE_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	LL1160
ORF Names:	L0091

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Homoserine kinase HAMAP MF_00384		PRO_0000156578
Regions
	Nucleotide binding	84 – 94	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CGD7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EF0B2BD785E74D6B	FASTA	296	31,919
	10 20 30 40 50 60 MKIIVPATSA NLGAGFDSIG IAVNLYLTVE VLEESNDWKI DHDLGNNIPT DEKNLLLTTL 70 80 90 100 110 120 SAVLKAKNSS LSAKYHLKMT SEVPLARGLG SSSSVIIAGI ELANQLAKLN LTTEEKLELA 130 140 150 160 170 180 CEIEGHPDNV APALLGNLVI ASTVADKTNY VSSDFPSCKL LAFVPDYELK TVESRKVLPK 190 200 210 220 230 240 ELAYKEAVAA SSIANVLTAS LLTKNLKVAG QMIESDHFHE NYRASLVPEL KILREIGHEF 250 260 270 280 290 GAYGTYLSGA GPTVMLLLPD DKLNLLTEKI NEQNLSGQLY SLEVDSNGLQ VKESVL

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05258.1.
PIR	H86769.
RefSeq	NP_267316.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CGD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114802.
GenomeReviews	Gene locus LL1160 in contig AE005176_GR.
KEGG	lla:L0091.
NMPDR	fig|272623.1.peg.1194.
PATRIC	22294664. VBILacLac136773_1240.
Phylogenomic databases
HOGENOM	HBG646290.
OMA	NNALMST.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	LLAC272623:L0091-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_LACLA

Accession

Primary (citable) accession number: Q9CGD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program