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Protein

DNA topoisomerase 1

Gene

topA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi32Magnesium 1; catalyticUniRule annotation1
Metal bindingi105Magnesium 1; catalyticUniRule annotation1
Metal bindingi105Magnesium 2UniRule annotation1
Metal bindingi107Magnesium 2UniRule annotation1
Active sitei321O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri595 – 621C4-type 1Add BLAST27
Zinc fingeri635 – 663C4-type 2Add BLAST29
Zinc fingeri676 – 702C4-type 3; atypicalAdd BLAST27

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:LL1230
ORF Names:L0298
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451531 – 710DNA topoisomerase 1Add BLAST710

Proteomic databases

PaxDbiQ9CG80.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with DNAUniRule annotation1
Sitei162Interaction with DNAUniRule annotation1
Sitei163Interaction with DNAUniRule annotation1
Sitei166Interaction with DNAUniRule annotation1
Sitei171Interaction with DNAUniRule annotation1
Sitei178Interaction with DNAUniRule annotation1
Sitei323Interaction with DNAUniRule annotation1
Sitei506Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi272623.L0298.

Structurei

3D structure databases

ProteinModelPortaliQ9CG80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 136ToprimUniRule annotationAdd BLAST111

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni186 – 191Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri595 – 621C4-type 1Add BLAST27
Zinc fingeri635 – 663C4-type 2Add BLAST29
Zinc fingeri676 – 702C4-type 3; atypicalAdd BLAST27

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG0551. LUCA.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CG80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTSTKSKTK TTTKKKTTRK RVTPGKNLVI VESPAKAKTI EKYLGRNYKV
60 70 80 90 100
VASVGHIRDL KKSTMSVDIE NDYEPQYINI RGKAPLINSL KKEAKAAKAV
110 120 130 140 150
YLASDPDREG EAISWHLAHI LNLPLEEKNR VVFNEITKDA VKNAFKEPRQ
160 170 180 190 200
IDVDLVDAQQ ARRVLDRLVG YSISPILWKK VKKGLSAGRV QSIALKLIVD
210 220 230 240 250
RENEINAFIP EEYWSIDGEF KKGTKKFKAS FWGIDGKKHP LKTNEDVIKV
260 270 280 290 300
MERLDGPDFN VDKVEKKERR RNAPLPYTTS SMQQDAANKL NFRTRKTMMV
310 320 330 340 350
AQQLYEGLTL GSQGHQGLIT YMRTDSTRIS PVAQSAAHNY IADQFGEKYS
360 370 380 390 400
KHGSKVKNAS GAQDAHEAIR PSNVFNTPDA IAKYLDKDQL KLYTLIWNRF
410 420 430 440 450
VASQMVAAIF DTVKVNLSQN GVIFIANGSQ VKFDGYLRIY NDSDKSNMLP
460 470 480 490 500
EMEENETVKK ANVKPEQHFT QPPARYSEAT LIKTLEENGV GRPSTYAPTL
510 520 530 540 550
ETIQKRYYVR LSAKRFEPTE LGEIVNKLIV EFFPNIVNTE FTAEMEKDLD
560 570 580 590 600
EVEEGKRQWI EVVDQFYKPF AKELENAEEG MEKIQIKDEP AGFDCDVCGS
610 620 630 640 650
PMVIKLGRFG KFYACSNFPD CRNTKAIVKE IGVKCPLCHE GNIIERKTKK
660 670 680 690 700
NRIFYGCDRY PDCEFTSWDK PIGRDCPKSG HFLVEKKVRG GGKQVVCSND
710
ECDYQEEKQK
Length:710
Mass (Da):80,741
Last modified:June 20, 2001 - v1
Checksum:i1268E706C38A59EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1.
PIRiF86778.
RefSeqiNP_267386.1. NC_002662.1.
WP_003131148.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05328; AAK05328; L0298.
GeneIDi1114880.
KEGGilla:L0298.
PATRICi22294848. VBILacLac136773_1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1.
PIRiF86778.
RefSeqiNP_267386.1. NC_002662.1.
WP_003131148.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CG80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0298.

Proteomic databases

PaxDbiQ9CG80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05328; AAK05328; L0298.
GeneIDi1114880.
KEGGilla:L0298.
PATRICi22294848. VBILacLac136773_1332.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG0551. LUCA.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1_LACLA
AccessioniPrimary (citable) accession number: Q9CG80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.