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Q9CG80

- TOP1_LACLA

UniProt

Q9CG80 - TOP1_LACLA

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Protein

DNA topoisomerase 1

Gene
topA, LL1230, L0298
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi32 – 321Magnesium 1; catalytic By similarity
Sitei56 – 561Interaction with DNA By similarity
Metal bindingi105 – 1051Magnesium 1; catalytic By similarity
Metal bindingi105 – 1051Magnesium 2 By similarity
Metal bindingi107 – 1071Magnesium 2 By similarity
Sitei162 – 1621Interaction with DNA By similarity
Sitei163 – 1631Interaction with DNA By similarity
Sitei166 – 1661Interaction with DNA By similarity
Sitei171 – 1711Interaction with DNA By similarity
Sitei178 – 1781Interaction with DNA By similarity
Active sitei321 – 3211O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei323 – 3231Interaction with DNA By similarity
Sitei506 – 5061Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri595 – 62127C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri635 – 66329C4-type 2UniRule annotationAdd
BLAST
Zinc fingeri676 – 70227C4-type 3; atypicalUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1319-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:LL1230
ORF Names:L0298
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710DNA topoisomerase 1UniRule annotationPRO_0000145153Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272623.L0298.

Structurei

3D structure databases

ProteinModelPortaliQ9CG80.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 136111ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri595 – 62127C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri635 – 66329C4-type 2UniRule annotationAdd
BLAST
Zinc fingeri676 – 70227C4-type 3; atypicalUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiWINKAKK.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CG80-1 [UniParc]FASTAAdd to Basket

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MPTSTKSKTK TTTKKKTTRK RVTPGKNLVI VESPAKAKTI EKYLGRNYKV    50
VASVGHIRDL KKSTMSVDIE NDYEPQYINI RGKAPLINSL KKEAKAAKAV 100
YLASDPDREG EAISWHLAHI LNLPLEEKNR VVFNEITKDA VKNAFKEPRQ 150
IDVDLVDAQQ ARRVLDRLVG YSISPILWKK VKKGLSAGRV QSIALKLIVD 200
RENEINAFIP EEYWSIDGEF KKGTKKFKAS FWGIDGKKHP LKTNEDVIKV 250
MERLDGPDFN VDKVEKKERR RNAPLPYTTS SMQQDAANKL NFRTRKTMMV 300
AQQLYEGLTL GSQGHQGLIT YMRTDSTRIS PVAQSAAHNY IADQFGEKYS 350
KHGSKVKNAS GAQDAHEAIR PSNVFNTPDA IAKYLDKDQL KLYTLIWNRF 400
VASQMVAAIF DTVKVNLSQN GVIFIANGSQ VKFDGYLRIY NDSDKSNMLP 450
EMEENETVKK ANVKPEQHFT QPPARYSEAT LIKTLEENGV GRPSTYAPTL 500
ETIQKRYYVR LSAKRFEPTE LGEIVNKLIV EFFPNIVNTE FTAEMEKDLD 550
EVEEGKRQWI EVVDQFYKPF AKELENAEEG MEKIQIKDEP AGFDCDVCGS 600
PMVIKLGRFG KFYACSNFPD CRNTKAIVKE IGVKCPLCHE GNIIERKTKK 650
NRIFYGCDRY PDCEFTSWDK PIGRDCPKSG HFLVEKKVRG GGKQVVCSND 700
ECDYQEEKQK 710
Length:710
Mass (Da):80,741
Last modified:June 20, 2001 - v1
Checksum:i1268E706C38A59EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1.
PIRiF86778.
RefSeqiNP_267386.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05328; AAK05328; L0298.
GeneIDi1114880.
KEGGilla:L0298.
PATRICi22294848. VBILacLac136773_1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1 .
PIRi F86778.
RefSeqi NP_267386.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q9CG80.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L0298.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK05328 ; AAK05328 ; L0298 .
GeneIDi 1114880.
KEGGi lla:L0298.
PATRICi 22294848. VBILacLac136773_1332.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi WINKAKK.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci LLAC272623:GHSH-1319-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiTOP1_LACLA
AccessioniPrimary (citable) accession number: Q9CG80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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