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Q9CG80

- TOP1_LACLA

UniProt

Q9CG80 - TOP1_LACLA

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi32 – 321Magnesium 1; catalyticUniRule annotation
Sitei56 – 561Interaction with DNAUniRule annotation
Metal bindingi105 – 1051Magnesium 1; catalyticUniRule annotation
Metal bindingi105 – 1051Magnesium 2UniRule annotation
Metal bindingi107 – 1071Magnesium 2UniRule annotation
Sitei162 – 1621Interaction with DNAUniRule annotation
Sitei163 – 1631Interaction with DNAUniRule annotation
Sitei166 – 1661Interaction with DNAUniRule annotation
Sitei171 – 1711Interaction with DNAUniRule annotation
Sitei178 – 1781Interaction with DNAUniRule annotation
Active sitei321 – 3211O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei323 – 3231Interaction with DNAUniRule annotation
Sitei506 – 5061Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri595 – 62127C4-type 1Add
BLAST
Zinc fingeri635 – 66329C4-type 2Add
BLAST
Zinc fingeri676 – 70227C4-type 3; atypicalAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1319-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:LL1230
ORF Names:L0298
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710DNA topoisomerase 1PRO_0000145153Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272623.L0298.

Structurei

3D structure databases

ProteinModelPortaliQ9CG80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 136111ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri595 – 62127C4-type 1Add
BLAST
Zinc fingeri635 – 66329C4-type 2Add
BLAST
Zinc fingeri676 – 70227C4-type 3; atypicalAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiWINKAKK.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CG80-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTSTKSKTK TTTKKKTTRK RVTPGKNLVI VESPAKAKTI EKYLGRNYKV
60 70 80 90 100
VASVGHIRDL KKSTMSVDIE NDYEPQYINI RGKAPLINSL KKEAKAAKAV
110 120 130 140 150
YLASDPDREG EAISWHLAHI LNLPLEEKNR VVFNEITKDA VKNAFKEPRQ
160 170 180 190 200
IDVDLVDAQQ ARRVLDRLVG YSISPILWKK VKKGLSAGRV QSIALKLIVD
210 220 230 240 250
RENEINAFIP EEYWSIDGEF KKGTKKFKAS FWGIDGKKHP LKTNEDVIKV
260 270 280 290 300
MERLDGPDFN VDKVEKKERR RNAPLPYTTS SMQQDAANKL NFRTRKTMMV
310 320 330 340 350
AQQLYEGLTL GSQGHQGLIT YMRTDSTRIS PVAQSAAHNY IADQFGEKYS
360 370 380 390 400
KHGSKVKNAS GAQDAHEAIR PSNVFNTPDA IAKYLDKDQL KLYTLIWNRF
410 420 430 440 450
VASQMVAAIF DTVKVNLSQN GVIFIANGSQ VKFDGYLRIY NDSDKSNMLP
460 470 480 490 500
EMEENETVKK ANVKPEQHFT QPPARYSEAT LIKTLEENGV GRPSTYAPTL
510 520 530 540 550
ETIQKRYYVR LSAKRFEPTE LGEIVNKLIV EFFPNIVNTE FTAEMEKDLD
560 570 580 590 600
EVEEGKRQWI EVVDQFYKPF AKELENAEEG MEKIQIKDEP AGFDCDVCGS
610 620 630 640 650
PMVIKLGRFG KFYACSNFPD CRNTKAIVKE IGVKCPLCHE GNIIERKTKK
660 670 680 690 700
NRIFYGCDRY PDCEFTSWDK PIGRDCPKSG HFLVEKKVRG GGKQVVCSND
710
ECDYQEEKQK
Length:710
Mass (Da):80,741
Last modified:June 20, 2001 - v1
Checksum:i1268E706C38A59EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1.
PIRiF86778.
RefSeqiNP_267386.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05328; AAK05328; L0298.
GeneIDi1114880.
KEGGilla:L0298.
PATRICi22294848. VBILacLac136773_1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005176 Genomic DNA. Translation: AAK05328.1 .
PIRi F86778.
RefSeqi NP_267386.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q9CG80.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L0298.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK05328 ; AAK05328 ; L0298 .
GeneIDi 1114880.
KEGGi lla:L0298.
PATRICi 22294848. VBILacLac136773_1332.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi WINKAKK.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci LLAC272623:GHSH-1319-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiTOP1_LACLA
AccessioniPrimary (citable) accession number: Q9CG80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3