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Q9CG80 (TOP1_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:LL1230
ORF Names:L0298
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length710 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 710710DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145153

Regions

Domain26 – 136111Toprim
Zinc finger595 – 62127C4-type 1 HAMAP-Rule MF_00952
Zinc finger635 – 66329C4-type 2 HAMAP-Rule MF_00952
Zinc finger676 – 70227C4-type 3; atypical HAMAP-Rule MF_00952
Region186 – 1916Interaction with DNA By similarity

Sites

Active site3211O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding321Magnesium 1; catalytic By similarity
Metal binding1051Magnesium 1; catalytic By similarity
Metal binding1051Magnesium 2 By similarity
Metal binding1071Magnesium 2 By similarity
Site561Interaction with DNA By similarity
Site1621Interaction with DNA By similarity
Site1631Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1711Interaction with DNA By similarity
Site1781Interaction with DNA By similarity
Site3231Interaction with DNA By similarity
Site5061Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CG80 [UniParc].

Last modified June 20, 2001. Version 1.
Checksum: 1268E706C38A59EB

FASTA71080,741
        10         20         30         40         50         60 
MPTSTKSKTK TTTKKKTTRK RVTPGKNLVI VESPAKAKTI EKYLGRNYKV VASVGHIRDL 

        70         80         90        100        110        120 
KKSTMSVDIE NDYEPQYINI RGKAPLINSL KKEAKAAKAV YLASDPDREG EAISWHLAHI 

       130        140        150        160        170        180 
LNLPLEEKNR VVFNEITKDA VKNAFKEPRQ IDVDLVDAQQ ARRVLDRLVG YSISPILWKK 

       190        200        210        220        230        240 
VKKGLSAGRV QSIALKLIVD RENEINAFIP EEYWSIDGEF KKGTKKFKAS FWGIDGKKHP 

       250        260        270        280        290        300 
LKTNEDVIKV MERLDGPDFN VDKVEKKERR RNAPLPYTTS SMQQDAANKL NFRTRKTMMV 

       310        320        330        340        350        360 
AQQLYEGLTL GSQGHQGLIT YMRTDSTRIS PVAQSAAHNY IADQFGEKYS KHGSKVKNAS 

       370        380        390        400        410        420 
GAQDAHEAIR PSNVFNTPDA IAKYLDKDQL KLYTLIWNRF VASQMVAAIF DTVKVNLSQN 

       430        440        450        460        470        480 
GVIFIANGSQ VKFDGYLRIY NDSDKSNMLP EMEENETVKK ANVKPEQHFT QPPARYSEAT 

       490        500        510        520        530        540 
LIKTLEENGV GRPSTYAPTL ETIQKRYYVR LSAKRFEPTE LGEIVNKLIV EFFPNIVNTE 

       550        560        570        580        590        600 
FTAEMEKDLD EVEEGKRQWI EVVDQFYKPF AKELENAEEG MEKIQIKDEP AGFDCDVCGS 

       610        620        630        640        650        660 
PMVIKLGRFG KFYACSNFPD CRNTKAIVKE IGVKCPLCHE GNIIERKTKK NRIFYGCDRY 

       670        680        690        700        710 
PDCEFTSWDK PIGRDCPKSG HFLVEKKVRG GGKQVVCSND ECDYQEEKQK 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005176 Genomic DNA. Translation: AAK05328.1.
PIRF86778.
RefSeqNP_267386.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CG80.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05328; AAK05328; L0298.
GeneID1114880.
KEGGlla:L0298.
PATRIC22294848. VBILacLac136773_1332.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1319-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_LACLA
AccessionPrimary (citable) accession number: Q9CG80
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: February 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families