Q9CG65 (GLPO_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glycerophosphate oxidase
EC=1.1.3.21

Alternative name(s):
Glycerol-3-phosphate oxidase

Gene names

Name:	glpO
Synonyms:	glpD
Ordered Locus Names:	LL1245
ORF Names:	L0013

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Caution	As L.lactis is unable to produce acid from glycerol, the significance and/or function of the glpO gene in this organism is at present unknown.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Alpha-glycerophosphate oxidase		PRO_0000126109
Regions
	Nucleotide binding	21 – 49	29	FAD Potential
Experimental info
	Sequence conflict	152	1	R → H in AAB63270. Ref.2
	Sequence conflict	253	1	K → R in AAB63270. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9CG65 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E7A31EA7A2A89236	FASTA	609	67,310
	10 20 30 40 50 60 MAFSKKTRQE AITKIQNEEM DLLVIGGGIT GAGLTLQAAA AGMKVAVLEM QDFSEGTSSR 70 80 90 100 110 120 STKLVHGGIR YLKNFDVEVV SDTVSERAVV QGIAPHIPKP DPMLLPIYDD EGKTTFDMFS 130 140 150 160 170 180 VKIAMDLYDR LAGVDEDSPY ANYTISPEEV LRREPLIKKK GLQGAGVYLD YRNNDARLVI 190 200 210 220 230 240 DNIKKAVEDG AQAISKMKVI DFIYTDGQIS GIRARDLLTD QVIEVKAKLV INTSGPWVDK 250 260 270 280 290 300 IRYLNFTRPI VPKMRPTKGV HLVVDAAKLP VPQPTYFDTG KHDKRMVFAI PRENKTYFGT 310 320 330 340 350 360 TDTDYHGDFT DPKVTQEDVD YLLDVINFRY PEANITINDI EASWAGLRPL LGGNSGSDYN 370 380 390 400 410 420 GGDNGAVSET SFNAVVEAVL RYKNKTATKA EVEHLLNNME SSLSEKGDAP SSVSRGSSLE 430 440 450 460 470 480 RESDGLITLA GGKITDYRKM AAGAMELICQ LLEEDFGLKY EPIDSKKYQI SGGEFDPTKV 490 500 510 520 530 540 EEVVAENMKV GVAAGLTEEE AKYIADFYGM NALQVFAYAS EMEAYEGLSL AESARLCYAL 550 560 570 580 590 600 EDEMILTPVD YLLRRTNHIL FMREGVDAIK VHVVNAMADD LGWSAEEKAE QEKALEEALR ESDLSDLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[2]	"Strain-specific differentiation of lactococci in mixed starter culture populations using randomly amplified polymorphic DNA-derived probes." Erlandson K., Batt C.A. Appl. Environ. Microbiol. 63:2702-2707(1997) [PubMed: 9212417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-253. Strain: 210.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05343.1. AF001829 Genomic DNA. Translation: AAB63270.1.
PIR	E86780.
RefSeq	NP_267401.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CG65.
SMR	Q9CG65. Positions 3-608.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114894.
GenomeReviews	Gene locus LL1245 in contig AE005176_GR.
KEGG	lla:L0013.
NMPDR	fig|272623.1.peg.1280.
PATRIC	22294876. VBILacLac136773_1346.
Phylogenomic databases
HOGENOM	HBG720894.
OMA	EWMLLPI.
ProtClustDB	CLSK2750091.
Enzyme and pathway databases
BioCyc	LLAC272623:L0013-MONOMER.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
KO	K00111.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_LACLA

Accession

Primary (citable) accession number: Q9CG65
Secondary accession number(s): O07381

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program