Q9CG20 (DCE_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate decarboxylase
Short name=GAD
EC=4.1.1.15

Gene names

Name:	gadB
Ordered Locus Names:	LL1290
ORF Names:	L123581

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance By similarity.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.
Biophysicochemical properties	pH dependence: Optimum pH is 4.7. Active at acidic pHs but inactive in the neutral pH range. Temperature dependence: Active up to 70 degrees Celsius at pH 4.1.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Glutamate decarboxylase		PRO_0000146986
Amino acid modifications
	Modified residue	277	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CG20 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BCDC732253E473C2	FASTA	466	53,927
	10 20 30 40 50 60 MLYGKENRDE AEFLEPIFGS ESEQVDLPKY KLAQQSIEPR VAYQLVQDEM LDEGNARLNL 70 80 90 100 110 120 ATFCQTYMEP EAVKLMSQTL EKNAIDKSEY PRTTEIENRC VNMIADLWNA SEKEKFMGTS 130 140 150 160 170 180 TIGSSEACML GGMAMKFSWR KRAEKLGLDI NAKKPNLVIS SGYQVCWEKF CIYWDIEMRE 190 200 210 220 230 240 VPMDKEHMSI NLDKVMDYVD EYTIGVVGIM GITYTGRYDD IKALDNLIEE YNKQTDYKVY 250 260 270 280 290 300 IHVDAASGGL YAPFVEPELE WDFRLKNVIS INTSGHKYGL VYPGVGWVLW RDKKYLPEEL 310 320 330 340 350 360 IFKVSYLGGE LPTMAINFSH SASQLIGQYY NFVRYGFDGY KAIHERTHKV AMFLAKEIEK 370 380 390 400 410 420 TGMFEIMNDG SQLPIVCYKL KEDSNRGWNL YDLADRLLMK GWQVPAYPLP KNLENEIIQR 430 440 450 460 LVIRADFGMN MAFNYVQDMQ EAIEALNKAH ILYHEEPENK TYGFTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lactococcus lactis contains only one glutamate decarboxylase gene." Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H. Microbiology 145:1375-1380(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION. Strain: 01-7.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010789 Genomic DNA. Translation: BAA24585.1. AE005176 Genomic DNA. Translation: AAK05388.1.
PIR	B86786.
RefSeq	NP_267446.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CG20.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L123581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05388; AAK05388; L123581.
GeneID	1114939.
KEGG	lla:L123581.
PATRIC	22294970. VBILacLac136773_1393.
Phylogenomic databases
eggNOG	COG0076.
HOGENOM	HOG000070228.
KO	K01580.
OMA	PMDKEHM.
ProtClustDB	CLSK876864.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-1419-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCE_LACLA

Accession

Primary (citable) accession number: Q9CG20
Secondary accession number(s): O50645

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program