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Protein

Glutamate decarboxylase

Gene

gadB

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance (By similarity).By similarity

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

pH dependencei

Optimum pH is 4.7. Active at acidic pHs but inactive in the neutral pH range.

Temperature dependencei

Active up to 70 degrees Celsius at pH 4.1.

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glutamate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1378-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase (EC:4.1.1.15)
Short name:
GAD
Gene namesi
Name:gadB
Ordered Locus Names:LL1290
ORF Names:L123581
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Glutamate decarboxylasePRO_0000146986Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei277 – 2771N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi272623.L123581.

Structurei

3D structure databases

ProteinModelPortaliQ9CG20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000070228.
KOiK01580.
OMAiDPDLVWD.
OrthoDBiEOG6TFCPW.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CG20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYGKENRDE AEFLEPIFGS ESEQVDLPKY KLAQQSIEPR VAYQLVQDEM
60 70 80 90 100
LDEGNARLNL ATFCQTYMEP EAVKLMSQTL EKNAIDKSEY PRTTEIENRC
110 120 130 140 150
VNMIADLWNA SEKEKFMGTS TIGSSEACML GGMAMKFSWR KRAEKLGLDI
160 170 180 190 200
NAKKPNLVIS SGYQVCWEKF CIYWDIEMRE VPMDKEHMSI NLDKVMDYVD
210 220 230 240 250
EYTIGVVGIM GITYTGRYDD IKALDNLIEE YNKQTDYKVY IHVDAASGGL
260 270 280 290 300
YAPFVEPELE WDFRLKNVIS INTSGHKYGL VYPGVGWVLW RDKKYLPEEL
310 320 330 340 350
IFKVSYLGGE LPTMAINFSH SASQLIGQYY NFVRYGFDGY KAIHERTHKV
360 370 380 390 400
AMFLAKEIEK TGMFEIMNDG SQLPIVCYKL KEDSNRGWNL YDLADRLLMK
410 420 430 440 450
GWQVPAYPLP KNLENEIIQR LVIRADFGMN MAFNYVQDMQ EAIEALNKAH
460
ILYHEEPENK TYGFTH
Length:466
Mass (Da):53,927
Last modified:May 31, 2001 - v1
Checksum:iBCDC732253E473C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010789 Genomic DNA. Translation: BAA24585.1.
AE005176 Genomic DNA. Translation: AAK05388.1.
PIRiB86786.
RefSeqiNP_267446.1. NC_002662.1.
WP_010905870.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05388; AAK05388; L123581.
GeneIDi1114939.
KEGGilla:L123581.
PATRICi22294970. VBILacLac136773_1393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010789 Genomic DNA. Translation: BAA24585.1.
AE005176 Genomic DNA. Translation: AAK05388.1.
PIRiB86786.
RefSeqiNP_267446.1. NC_002662.1.
WP_010905870.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CG20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L123581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05388; AAK05388; L123581.
GeneIDi1114939.
KEGGilla:L123581.
PATRICi22294970. VBILacLac136773_1393.

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000070228.
KOiK01580.
OMAiDPDLVWD.
OrthoDBiEOG6TFCPW.

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1378-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Lactococcus lactis contains only one glutamate decarboxylase gene."
    Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H.
    Microbiology 145:1375-1380(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION.
    Strain: 01-7.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiDCE_LACLA
AccessioniPrimary (citable) accession number: Q9CG20
Secondary accession number(s): O50645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 25, 2001
Last sequence update: May 31, 2001
Last modified: January 6, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.