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Q9CG20 (DCE_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate decarboxylase

Short name=GAD
EC=4.1.1.15
Gene names
Name:gadB
Ordered Locus Names:LL1290
ORF Names:L123581
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance By similarity.

Catalytic activity

L-glutamate = 4-aminobutanoate + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Sequence similarities

Belongs to the group II decarboxylase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.7. Active at acidic pHs but inactive in the neutral pH range.

Temperature dependence:

Active up to 70 degrees Celsius at pH 4.1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Glutamate decarboxylase
PRO_0000146986

Amino acid modifications

Modified residue2771N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CG20 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: BCDC732253E473C2

FASTA46653,927
        10         20         30         40         50         60 
MLYGKENRDE AEFLEPIFGS ESEQVDLPKY KLAQQSIEPR VAYQLVQDEM LDEGNARLNL 

        70         80         90        100        110        120 
ATFCQTYMEP EAVKLMSQTL EKNAIDKSEY PRTTEIENRC VNMIADLWNA SEKEKFMGTS 

       130        140        150        160        170        180 
TIGSSEACML GGMAMKFSWR KRAEKLGLDI NAKKPNLVIS SGYQVCWEKF CIYWDIEMRE 

       190        200        210        220        230        240 
VPMDKEHMSI NLDKVMDYVD EYTIGVVGIM GITYTGRYDD IKALDNLIEE YNKQTDYKVY 

       250        260        270        280        290        300 
IHVDAASGGL YAPFVEPELE WDFRLKNVIS INTSGHKYGL VYPGVGWVLW RDKKYLPEEL 

       310        320        330        340        350        360 
IFKVSYLGGE LPTMAINFSH SASQLIGQYY NFVRYGFDGY KAIHERTHKV AMFLAKEIEK 

       370        380        390        400        410        420 
TGMFEIMNDG SQLPIVCYKL KEDSNRGWNL YDLADRLLMK GWQVPAYPLP KNLENEIIQR 

       430        440        450        460 
LVIRADFGMN MAFNYVQDMQ EAIEALNKAH ILYHEEPENK TYGFTH 

« Hide

References

« Hide 'large scale' references
[1]"Lactococcus lactis contains only one glutamate decarboxylase gene."
Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H.
Microbiology 145:1375-1380(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION.
Strain: 01-7.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB010789 Genomic DNA. Translation: BAA24585.1.
AE005176 Genomic DNA. Translation: AAK05388.1.
PIRB86786.
RefSeqNP_267446.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CG20.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L123581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05388; AAK05388; L123581.
GeneID1114939.
KEGGlla:L123581.
PATRIC22294970. VBILacLac136773_1393.

Phylogenomic databases

eggNOGCOG0076.
HOGENOMHOG000070228.
KOK01580.
OMAPTFQINF.
OrthoDBEOG6TFCPW.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1378-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
InterProIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDCE_LACLA
AccessionPrimary (citable) accession number: Q9CG20
Secondary accession number(s): O50645
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families