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Q9CG20

- DCE_LACLA

UniProt

Q9CG20 - DCE_LACLA

Protein

Glutamate decarboxylase

Gene

gadB

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance By similarity.By similarity

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    pH dependencei

    Optimum pH is 4.7. Active at acidic pHs but inactive in the neutral pH range.

    Temperature dependencei

    Active up to 70 degrees Celsius at pH 4.1.

    GO - Molecular functioni

    1. glutamate decarboxylase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glutamate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-1378-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase (EC:4.1.1.15)
    Short name:
    GAD
    Gene namesi
    Name:gadB
    Ordered Locus Names:LL1290
    ORF Names:L123581
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 466466Glutamate decarboxylasePRO_0000146986Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei277 – 2771N6-(pyridoxal phosphate)lysineBy similarity

    Interactioni

    Protein-protein interaction databases

    STRINGi272623.L123581.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CG20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    HOGENOMiHOG000070228.
    KOiK01580.
    OMAiPTFQINF.
    OrthoDBiEOG6TFCPW.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9CG20-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLYGKENRDE AEFLEPIFGS ESEQVDLPKY KLAQQSIEPR VAYQLVQDEM    50
    LDEGNARLNL ATFCQTYMEP EAVKLMSQTL EKNAIDKSEY PRTTEIENRC 100
    VNMIADLWNA SEKEKFMGTS TIGSSEACML GGMAMKFSWR KRAEKLGLDI 150
    NAKKPNLVIS SGYQVCWEKF CIYWDIEMRE VPMDKEHMSI NLDKVMDYVD 200
    EYTIGVVGIM GITYTGRYDD IKALDNLIEE YNKQTDYKVY IHVDAASGGL 250
    YAPFVEPELE WDFRLKNVIS INTSGHKYGL VYPGVGWVLW RDKKYLPEEL 300
    IFKVSYLGGE LPTMAINFSH SASQLIGQYY NFVRYGFDGY KAIHERTHKV 350
    AMFLAKEIEK TGMFEIMNDG SQLPIVCYKL KEDSNRGWNL YDLADRLLMK 400
    GWQVPAYPLP KNLENEIIQR LVIRADFGMN MAFNYVQDMQ EAIEALNKAH 450
    ILYHEEPENK TYGFTH 466
    Length:466
    Mass (Da):53,927
    Last modified:June 1, 2001 - v1
    Checksum:iBCDC732253E473C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010789 Genomic DNA. Translation: BAA24585.1.
    AE005176 Genomic DNA. Translation: AAK05388.1.
    PIRiB86786.
    RefSeqiNP_267446.1. NC_002662.1.
    WP_010905870.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK05388; AAK05388; L123581.
    GeneIDi1114939.
    KEGGilla:L123581.
    PATRICi22294970. VBILacLac136773_1393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB010789 Genomic DNA. Translation: BAA24585.1 .
    AE005176 Genomic DNA. Translation: AAK05388.1 .
    PIRi B86786.
    RefSeqi NP_267446.1. NC_002662.1.
    WP_010905870.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q9CG20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L123581.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK05388 ; AAK05388 ; L123581 .
    GeneIDi 1114939.
    KEGGi lla:L123581.
    PATRICi 22294970. VBILacLac136773_1393.

    Phylogenomic databases

    eggNOGi COG0076.
    HOGENOMi HOG000070228.
    KOi K01580.
    OMAi PTFQINF.
    OrthoDBi EOG6TFCPW.

    Enzyme and pathway databases

    BioCyci LLAC272623:GHSH-1378-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    InterProi IPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    PANTHERi PTHR11999:SF1. PTHR11999:SF1. 1 hit.
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01788. Glu-decarb-GAD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Lactococcus lactis contains only one glutamate decarboxylase gene."
      Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H.
      Microbiology 145:1375-1380(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, CHARACTERIZATION.
      Strain: 01-7.
    2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiDCE_LACLA
    AccessioniPrimary (citable) accession number: Q9CG20
    Secondary accession number(s): O50645
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3