Q9CFY8 (PANE_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative 2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Ordered Locus Names:	LL1323
ORF Names:	L157055

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Putative 2-dehydropantoate 2-reductase		PRO_0000157313
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	187	1	Proton donor By similarity
	Binding site	105	1	NADP; via amide nitrogen By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Binding site	260	1	Substrate By similarity
	Binding site	273	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CFY8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1C7FC4547F081BAD	FASTA	312	34,444
	10 20 30 40 50 60 MRITIAGAGA MGSRFGLMLH KGGNEVTLID GWPEHVKAIK EHGLRANYNG EELTAHLSVE 70 80 90 100 110 120 LQSEISSKEK TDLIILFTKA MQLDKMLQDI KPLIDEHTKV LCLLNGIGHE DTIEKYVSKN 130 140 150 160 170 180 NIFIGNTMWT AGLEGPGKAK LFGDGSVELQ NLISGEEETA KKLAEILSES GLNAKYSNNI 190 200 210 220 230 240 HYSIYRKACV NGTMNGLCTI LDTNMAGLGE TKPAHDMVVT IVNEFAAVAK FENVNLDIAE 250 260 270 280 290 300 VVQHVETCFD PSTIGLHYPS MYQDLIKNNR LTEIDYINGA VSRKGKKYNV ATPYCDFLTQ 310 LVHSKEELLK AK

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05421.1.
PIR	C86790.
RefSeq	NP_267479.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CFY8.
SMR	Q9CFY8. Positions 1-312.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114972.
GenomeReviews	Gene locus LL1323 in contig AE005176_GR.
KEGG	lla:L157055.
NMPDR	fig|272623.1.peg.1358.
PATRIC	22295038. VBILacLac136773_1427.
Phylogenomic databases
HOGENOM	HBG668370.
OMA	TIYNCAL.
ProtClustDB	PRK06522.
Enzyme and pathway databases
BioCyc	LLAC272623:L157055-MONOMER.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00077.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. ApbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_LACLA

Accession

Primary (citable) accession number: Q9CFY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program