Q9CFW8 (PYRDB_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Short name=DHOD B
Short name=DHODase B
Short name=DHOdehase B
EC=1.3.1.14

Alternative name(s):
Dihydrdoorotate oxidase B
Orotate reductase (NADH)

Gene names

Name:	pyrDB
Synonyms:	pydB
Ordered Locus Names:	LL1346
ORF Names:	L182555

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dihydroorotate to orotate with NAD+ as electron acceptor By similarity. HAMAP MF_00224
Catalytic activity	(S)-dihydroorotate + NAD+ = orotate + NADH. HAMAP MF_00224
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00224
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1. HAMAP MF_00224
Subunit structure	Heterotetramer of 2 PyrK and 2 PyrD type B subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00224.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro orotate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit HAMAP MF_00224		PRO_0000148395
Regions
	Nucleotide binding	48 – 49	2	FMN By similarity
	Nucleotide binding	248 – 249	2	FMN By similarity
	Nucleotide binding	270 – 271	2	FMN By similarity
	Region	72 – 76	5	Substrate binding By similarity
	Region	197 – 198	2	Substrate binding By similarity
Sites
	Active site	135	1	Nucleophile
	Binding site	24	1	FMN By similarity
	Binding site	48	1	Substrate By similarity
	Binding site	104	1	FMN By similarity
	Binding site	132	1	FMN By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	170	1	FMN By similarity
	Binding site	196	1	FMN; via carbonyl oxygen By similarity
	Binding site	222	1	FMN; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CFW8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 6FF419606B2E733C	FASTA	311	32,916
	10 20 30 40 50 60 MTENNRLSVK LPGLDLKNPI IPASGCFGFG EEYAKYYDLN KLGSIMVKAT TLHPRFGNPT 70 80 90 100 110 120 PRVAETASGM LNAIGLQNPG LEVIMAEKLP WLNENFPDLP IIANVAGSEE DDYVAVCAKI 130 140 150 160 170 180 GDAPNVKVIE LNISCPNVKH GGQAFGTDPD VAAALVKACK AVSKVPLYVK LSPNVTDIVP 190 200 210 220 230 240 IAKAVEAAGA DGLTMINTLM GVRFDLKTRK PVLANITGGL SGPAIKPVAL KLIHQVAQVV 250 260 270 280 290 300 DIPIIGMGGV ESAQDVLEMY MAGASAVAVG TANFADPFVC PKIIEKLPEV MDQYGIDSLE 310 NLIQEVKNSK K

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05444.1.
PIR	B86793.
RefSeq	NP_267502.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CFW8.
SMR	Q9CFW8. Positions 1-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114996.
GenomeReviews	Gene locus LL1346 in contig AE005176_GR.
KEGG	lla:L182555.
NMPDR	fig|272623.1.peg.1381.
PATRIC	22295088. VBILacLac136773_1452.
Phylogenomic databases
HOGENOM	HBG472415.
OMA	VALRMVW.
ProtClustDB	PRK07259.
Enzyme and pathway databases
BioCyc	LLAC272623:L182555-MONOMER. MetaCyc:MONOMER-14471.
Family and domain databases
HAMAP	MF_00224. DHO_dh_type1. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005720. Dihydroorotate_DH. IPR024920. Dihydroorotate_DH_1. IPR012135. Dihydroorotate_DH_1_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00226.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. PyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRDB_LACLA

Accession

Primary (citable) accession number: Q9CFW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program