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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (yphF)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541UniRule annotation
Binding sitei57 – 571ATPUniRule annotation
Binding sitei96 – 961ATPUniRule annotation
Metal bindingi98 – 981Magnesium 1UniRule annotation
Active sitei100 – 1001Proton acceptorUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Metal bindingi122 – 1221Magnesium 2UniRule annotation
Binding sitei245 – 2451SubstrateUniRule annotation
Metal bindingi275 – 2751Magnesium 2UniRule annotation
Binding sitei504 – 5041ATPUniRule annotation
Binding sitei541 – 5411ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi542 – 5421Magnesium 1UniRule annotation
Binding sitei544 – 5441SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1627-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:LL1530
ORF Names:L173921
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739Phosphoribosylformylglycinamidine synthase subunit PurLPRO_0000100461Add
BLAST

Proteomic databases

PaxDbiQ9CFE8.

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi272623.L173921.

Structurei

3D structure databases

ProteinModelPortaliQ9CFE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1024Substrate bindingUniRule annotation
Regioni319 – 3213Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JIU. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CFE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEMSPEQI QESKIYREWG LTDEEYLKIK DEILDGRLPN FTETGMYAVM
60 70 80 90 100
WSEHCCYKNS KPVLKKFPTT GPQVLMGPGE GAGVVDIGDD LAVVFKAESH
110 120 130 140 150
NHPSYVEPYE GAATGSGGII RDIFSMGARP IAILDSLRFG PIDNGKTRHI
160 170 180 190 200
VDQVTAGIAG YGNCIGIPTV GGEVAFDESY AGNPLVNVMC VGLIEHKHIQ
210 220 230 240 250
KGQAKGVGNS IFYVGAKTGR DGIHGASFAS KEFGSGSETQ RSAVQVGDPF
260 270 280 290 300
MEKLLLEACI EVIQNHGDIL VGIQDMGAAG LVSSTSEMAS KAGSGLRLNL
310 320 330 340 350
DNVPQRETEM IPYEMMLSES QERMVLCVKK GHEQEIIDLF KKYDLDAVNI
360 370 380 390 400
GQVTDDGFYT LYHKGEMVAH VPVDSLAEDA PTYYREAKVP ERIQKFTDSE
410 420 430 440 450
KYLPEITDSA VSEIFKKLLA QPTIASKKSI YETYDSRVMT NTVVAPGSDS
460 470 480 490 500
AVLRVRGTNK ALAMTTDCNA RYLYLDPEKG GAIAVAEAAR NIVASGGKPL
510 520 530 540 550
AITDCLNFGN PEKPEQFWEL TTAADGISRS CLALDTPVIS GNVSLYNETN
560 570 580 590 600
GSAILPTPMI GMVGLIENVK NITTQEFKKA GDLIVLVGQT FDDFSGSELQ
610 620 630 640 650
KMLIGEISGR IDFDLETEKI NQDFVLKAIT DGLVSSAHDL AEGGLAIALA
660 670 680 690 700
ESAFANGLGV DVKVDITNAQ LFSETQGRFI LSISPENQAT FEKLLTESSV
710 720 730
SGEVIGKVTD SGILEMNELS ISTDEAVSIY EGALPALMK
Length:739
Mass (Da):79,695
Last modified:June 1, 2001 - v1
Checksum:i5B09C007CDC9677E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05628.1.
PIRiB86816.
RefSeqiNP_267686.1. NC_002662.1.
WP_003130033.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05628; AAK05628; L173921.
GeneIDi1115187.
KEGGilla:L173921.
PATRICi22295468. VBILacLac136773_1641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05628.1.
PIRiB86816.
RefSeqiNP_267686.1. NC_002662.1.
WP_003130033.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CFE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L173921.

Proteomic databases

PaxDbiQ9CFE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05628; AAK05628; L173921.
GeneIDi1115187.
KEGGilla:L173921.
PATRICi22295468. VBILacLac136773_1641.

Phylogenomic databases

eggNOGiENOG4108JIU. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
OrthoDBiEOG6FNHHR.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
BioCyciLLAC272623:GHSH-1627-MONOMER.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiPURL_LACLA
AccessioniPrimary (citable) accession number: Q9CFE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: June 1, 2001
Last modified: November 11, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.