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Protein

Dihydroorotate dehydrogenase A (fumarate)

Gene

pyrDA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor.By similarity

Catalytic activityi

(S)-dihydroorotate + fumarate = orotate + succinate.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathway:iUMP biosynthesis via de novo pathway

This protein is involved in the pathway UMP biosynthesis via de novo pathway, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191FMNBy similarity
Binding sitei43 – 431SubstrateBy similarity
Binding sitei127 – 1271FMNBy similarity
Binding sitei127 – 1271SubstrateBy similarity
Active sitei130 – 1301Nucleophile
Binding sitei164 – 1641FMNBy similarity
Binding sitei192 – 1921FMN; via carbonyl oxygenBy similarity
Binding sitei221 – 2211FMN; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 442FMNBy similarity
Nucleotide bindingi249 – 2502FMNBy similarity
Nucleotide bindingi271 – 2722FMNBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1651-MONOMER.
SABIO-RKQ9CFC9.
UniPathwayiUPA00070.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase A (fumarate) (EC:1.3.98.1)
Short name:
DHOD A
Short name:
DHODase A
Short name:
DHOdehase A
Gene namesi
Name:pyrDA
Synonyms:pydA
Ordered Locus Names:LL1552
ORF Names:L192589
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Dihydroorotate dehydrogenase A (fumarate)PRO_0000148393Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272623.L192589.

Structurei

3D structure databases

ProteinModelPortaliQ9CFC9.
SMRiQ9CFC9. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 715Substrate bindingBy similarity
Regioni193 – 1942Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0167.
HOGENOMiHOG000225104.
KOiK00226.
OMAiNTIENAC.
OrthoDBiEOG6NPM9S.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00224. DHO_dh_type1.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CFC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKTTFANAE FANPFMNASG VHCMTTEDLE ELKASQAGAY ITKSSTLEKR
60 70 80 90 100
EGNPLPRYVD LELGSINSMG LPNLGFDYYL DYVLKNQKEK AQEAPIFFSI
110 120 130 140 150
AGMSAAENIA MLKKIQESNF SGITELNLSC PNVPGKPQLA YDFEATEKLL
160 170 180 190 200
KEVFTFFTKP LGVKLPPYFD LVHFDIMAEI LNQFPLTYVN SVNSIGNGLF
210 220 230 240 250
IDSEAESVVI KPKDGFGGIG GAYIKPTALA NVRAFYTRLK PEIKIIGTGG
260 270 280 290 300
IETGQDAFEH LLCGATMLQI GTALHKEGPA IFDRIIKELE EIMDKKGYQS
310
IADFHGKLKS L
Length:311
Mass (Da):34,231
Last modified:June 1, 2001 - v1
Checksum:i80B3619EC408BBA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05650.1.
PIRiH86818.
RefSeqiNP_267708.1. NC_002662.1.
WP_003130679.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05650; AAK05650; L192589.
GeneIDi1115211.
KEGGilla:L192589.
PATRICi22295518. VBILacLac136773_1666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK05650.1.
PIRiH86818.
RefSeqiNP_267708.1. NC_002662.1.
WP_003130679.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CFC9.
SMRiQ9CFC9. Positions 1-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L192589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05650; AAK05650; L192589.
GeneIDi1115211.
KEGGilla:L192589.
PATRICi22295518. VBILacLac136773_1666.

Phylogenomic databases

eggNOGiCOG0167.
HOGENOMiHOG000225104.
KOiK00226.
OMAiNTIENAC.
OrthoDBiEOG6NPM9S.

Enzyme and pathway databases

UniPathwayiUPA00070.
BioCyciLLAC272623:GHSH-1651-MONOMER.
SABIO-RKQ9CFC9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00224. DHO_dh_type1.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiPYRDA_LACLA
AccessioniPrimary (citable) accession number: Q9CFC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.