Q9CF80 (CARA_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LL1601
ORF Names:	L43866

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112284
Regions
	Domain	172 – 357	186	Glutamine amidotransferase type-1
	Region	1 – 168	168	CPSase HAMAP MF_01209
Sites
	Active site	247	1	Nucleophile By similarity
	Active site	331	1	By similarity
	Active site	333	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CF80 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D0F5D0AC9B2073E5	FASTA	357	39,658
	10 20 30 40 50 60 MSKRLLILED GTIFEGESLG ANLDVTGELV FNTGMTGYQE SITDQSYNGQ ILTFTYPIVG 70 80 90 100 110 120 NYGVNRDDYE SIHPTCKAVV VHEAACRPSN WRMQMSFDEF LKAKNIPGIT GVDTRAITKI 130 140 150 160 170 180 VREHGTMKAS LVQARDEVEH QMSQLQATVL PTNQVETSST TTAYPSPNTG RKVVVVDFGL 190 200 210 220 230 240 KHSILRELSK RECNLTVVPY NTSAQEILEM EPDGVMLTNG PGDPTDVPEA IEMIKEIQGK 250 260 270 280 290 300 IPIFGICLGH QLFSLANGAK TYKMKFGHRG FNHAVREIAT GRIDFTSQNH GYAVSSENLP 310 320 330 340 350 EDLMITHVEI NDDSVEGVRH KHFPAFSVQF HPDAAPGPHD ASYLFDDFMD LMDNFKK

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05699.1.
PIR	A86825.
RefSeq	NP_267757.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CF80.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1115261.
GenomeReviews	Gene locus LL1601 in contig AE005176_GR.
KEGG	lla:L43866.
NMPDR	fig|272623.1.peg.1643.
PATRIC	22295630. VBILacLac136773_1722.
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	LLAC272623:L43866-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LACLA

Accession

Primary (citable) accession number: Q9CF80

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program