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Reviewed, UniProtKB/Swiss-Prot Q9CF80 (CARA_LACLA)

Last modified February 9, 2010. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name: carA
Ordered Locus Names: LL1601
ORF Names: L43866
OrganismLactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]
Taxonomic identifier1360 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

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Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209

Sequence similarities

Belongs to the carA family.

Contains 1 glutamine amidotransferase type-1 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_0000112284

Regions

Domain172 – 357186Glutamine amidotransferase type-1
Region1 – 168168CPSase HAMAP MF_01209

Sites

Active site2471Nucleophile By similarity
Active site3311 By similarity
Active site3331 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9CF80-1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D0F5D0AC9B2073E5

FASTA35739,658
        10         20         30         40         50         60 
MSKRLLILED GTIFEGESLG ANLDVTGELV FNTGMTGYQE SITDQSYNGQ ILTFTYPIVG 

        70         80         90        100        110        120 
NYGVNRDDYE SIHPTCKAVV VHEAACRPSN WRMQMSFDEF LKAKNIPGIT GVDTRAITKI 

       130        140        150        160        170        180 
VREHGTMKAS LVQARDEVEH QMSQLQATVL PTNQVETSST TTAYPSPNTG RKVVVVDFGL 

       190        200        210        220        230        240 
KHSILRELSK RECNLTVVPY NTSAQEILEM EPDGVMLTNG PGDPTDVPEA IEMIKEIQGK 

       250        260        270        280        290        300 
IPIFGICLGH QLFSLANGAK TYKMKFGHRG FNHAVREIAT GRIDFTSQNH GYAVSSENLP 

       310        320        330        340        350 
EDLMITHVEI NDDSVEGVRH KHFPAFSVQF HPDAAPGPHD ASYLFDDFMD LMDNFKK

« Hide

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References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005176 Genomic DNA. Translation: AAK05699.1.
PIRA86825.
RefSeqNP_267757.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1115261.
GenomeReviewsGene locus LL1601 in contig AE005176_GR.
KEGGlla:L43866.
NMPDRfig|272623.1.peg.1643.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG286341.
OMAFTYPELG.

Enzyme and pathway databases

BioCycLLAC272623:L43866-MONOMER.
BRENDA6.3.5.5. 278870.

Family and domain databases

HAMAPMF_01209_B. CPSase_S_chain_B.
[Tree]
InterProIPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase_dom.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
[Graphical view]
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
PRINTSPR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCARA_LACLA
AccessionPrimary (citable) accession number: Q9CF80
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: June 1, 2001
Last modified: February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents