Q9CEY4 (PTC_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putrescine carbamoyltransferase
Short name=PTC
Short name=PTCase
EC=2.1.3.6

Alternative name(s):
Putrescine transcarbamoylase
Putrescine transcarbamylase

Gene names

Name:	ptcA
Synonyms:	otcA
Ordered Locus Names:	LL1700
ORF Names:	L0113

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine By similarity. HAMAP MF_02102
Catalytic activity	Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine. HAMAP MF_02102
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (transferase route): step 1/1. HAMAP MF_02102
Subunit structure	Homotrimer By similarity. HAMAP MF_02102
Subcellular location	Cytoplasm By similarity HAMAP MF_02102.
Sequence similarities	Belongs to the ATCase/OTCase family. PTCase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polyamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro putrescine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Putrescine carbamoyltransferase HAMAP MF_02102		PRO_0000112934
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Putrescine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CEY4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 94BC8CCBC4250A2D	FASTA	340	37,844
	10 20 30 40 50 60 MVTTNKRDFI TTEDYTKEEI LDIVTLGLKI KAAIKNGYYP PLLKNKSLGM IFQQTSTRTR 70 80 90 100 110 120 VSFETAMTQL GGHAEYLAPG QIQLGGHETI EDTSTVLSRL LDIIMARVDR HESVNNLAKH 130 140 150 160 170 180 TTIPVLNGMS DYNHPTQEVG DLTTMIEHLP AGKKLEDCKV VFVGDATQVC FSLGLIATKM 190 200 210 220 230 240 GMHFVHFGPK GYQLNEEHQA KLAANCEVSG GTYEVTDDEE SIVGADFLYT DVWYGLYDAE 250 260 270 280 290 300 LSEEERLAIF FPKYQVTPEM MAKAGAHTKF MHCLPASRGE EVVDAVIDGP NSICFDEAEN 310 320 330 340 RLTSIRALLV WLMSDYAEKN PYDLKAQAKA KAELEAYLAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[2]	"Retrieving sequences of enzymes experimentally characterized but erroneously annotated: the case of the putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Glansdorff N., Labedan B. BMC Genomics 5:52-52(2004) [PubMed: 15287962] [Abstract] Cited for: REANNOTATION AS A PTCASE.
[3]	"The difficulty of annotating genes: the case of putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Stalon V., Glansdorff N., Labedan B. Microbiology 150:3908-3911(2004) [PubMed: 15583144] [Abstract] Cited for: GENE NAME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05798.1.
PIR	D86837.
RefSeq	NP_267856.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CEY4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1115363.
GenomeReviews	Gene locus LL1700 in contig AE005176_GR.
KEGG	lla:L0113.
NMPDR	fig|272623.1.peg.1744.
PATRIC	22295838. VBILacLac136773_1824.
Phylogenomic databases
HOGENOM	HBG579429.
OMA	LGMIFQQ.
ProtClustDB	PRK02255.
Enzyme and pathway databases
BioCyc	LLAC272623:L0113-MONOMER.
Family and domain databases
HAMAP	MF_02102. PTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024903. PtcA. [Graphical view]
KO	K13252.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTC_LACLA

Accession

Primary (citable) accession number: Q9CEY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program