Reviewed, UniProtKB/Swiss-Prot Q9CEI4 (KPRS2_LACLA)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 2
      Short name=RPPK 2
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 2
      Short name=P-Rib-PP synthetase 2
      Short name=PRPP synthetase 2

Gene names

Name:	prs2
Synonyms:	prsB
Ordered Locus Names:	LL1857
ORF Names:	L109335

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis) [Complete proteome] [HAMAP]

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583		PRO_0000141147
Regions
	Region	216 – 229	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	132	1	Magnesium Potential
	Metal binding	134	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CEI4-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 88673E8BCBF3B56E	FASTA	321	35,278
	10 20 30 40 50 60 MVYSDSHLKL FALNSNPGLA EKISQFTGVP LGKLSSKQFS DGEIMINMEE SVRSQDIFIV 70 80 90 100 110 120 QSTSCPVNDH LWELLIMIDA CKRASARTIN VVIPYFGYAR QDRTATSREP ITAKLVADMI 130 140 150 160 170 180 VKAGADRLLT LDIHAVQVQG FFDIPVDNLF TVPLFADYYR KSGLFGDDVI VVAPKNSGIK 190 200 210 220 230 240 RARSLAEYLE SAIAIVDYED DDKNRENGYV IGNVSGKKVI LIDDILNTGV TFANAANVVR 250 260 270 280 290 300 EAGASEIYAV ASHGLFTSGA ADVLEKVDIK EILVTDSVVT NHRKPLNVKY LSAAEYLASA 310 320 ILRIHEGRPV SPLFEHEKPQ D

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005176 Genomic DNA. Translation: AAK05955.1.
PIR	A86857.
RefSeq	NP_268014.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBS based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1115522.
GenomeReviews	Gene locus LL1857 in contig AE005176_GR.
KEGG	lla:L109335.
NMPDR	fig|272623.1.peg.1903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CEI4.
OMA	NDHLWEL.
Enzyme and pathway databases
BioCyc	LLAC272623:L109335-MON.
BRENDA	2.7.6.1. 278870.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_LACLA

Accession

Primary (citable) accession number: Q9CEI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)