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Q9CEH8

- SYI_LACLA

UniProt

Q9CEH8 - SYI_LACLA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi914 – 9141ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-1970-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LL1863
    ORF Names:L0350
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098404Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272623.L0350.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CEH8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CEH8-1 [UniParc]FASTAAdd to Basket

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    MKIKDTLNLG KTAFPMRAGL PNKEPNWQKD WADATLYEKR QELNEGKPSF    50
    MLHDGPPYAN GNIHLGHSLN KISKDIIVRY KSMAGFRAPY VPGWDTHGLP 100
    IEQQLAKAGM KRKEMDLLDY LEECRKYAMK QVDMQRSDFK SLGVLADWDR 150
    PYLTLLPEYE AAQIRVFGKM AEKGYIYKGQ KPIYWSPSSE SSLAEAEIEY 200
    QDVRSASIFV AFKAKDTKGK LPEDVEFVIW TTTPWTIPSN LGIFAHPDYD 250
    YSVVAVNGRK FVIASEMLEA VAEKLEWENP EVLQTIKGSE LEYMVAKHPF 300
    YDRETLIMNA DYVTLDSGTG LVHVAPGHGE DDYFASRKYK LPVLSPIDNR 350
    GYYTDEAPGL EGLLYDEGNK VVSKWLEEKD ALLKLEFFTH SYPHDWRTKK 400
    PVIFRATPQW FASIDDFRQN ILDEVERVDW VIPWGKTRLF NMVRDRGDWV 450
    ISRQRAWGVP LPIFYGENGE PIITPETTEH VAKLFAEFGS KVWFEREAKD 500
    LLPEGFTHPA SPNGEFTKEK DIMDVWFDSG SSWNGVLNER DYLSFPADLY 550
    LEGSDQYRGW FNSSITTSVA VNGVAPYKAV LSQGFVLDGK GRKMSKSIGN 600
    TIVPKDVTKK FGADILRLWV ASIDTESDVR VSMDILSQVS EVYRKIRNTL 650
    RFLIANTSDF NPKEDAIDFA ELRPVDKYML VKFNELVKQI RTAYDNYSFM 700
    TVYKSIINFI TNDLSSFYLD FAKDVVYIEA ANSPERRSMQ TVMYVILKDL 750
    VKILVPILPH TAEETWTYLE HEPENFAYLA EMPEAAEIPG SEELLGNWQE 800
    FLDFRDKILK ALESAREAKL IGKSLEATVT IYPNEVVRTL LTAIDENVAQ 850
    LLIVSNFVVA NEPVNNAPES AMKFDDLAVL VEHAAGEVCD RCRRTDETVG 900
    HNANEHLKML CEHCAHIVET EFPEILEEGF ED 932
    Length:932
    Mass (Da):106,509
    Last modified:June 1, 2001 - v1
    Checksum:i85EF03E46A85D751
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005176 Genomic DNA. Translation: AAK05961.1.
    PIRiG86857.
    RefSeqiNP_268020.1. NC_002662.1.
    WP_010906172.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK05961; AAK05961; L0350.
    GeneIDi1115528.
    KEGGilla:L0350.
    PATRICi22296182. VBILacLac136773_1996.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005176 Genomic DNA. Translation: AAK05961.1 .
    PIRi G86857.
    RefSeqi NP_268020.1. NC_002662.1.
    WP_010906172.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali Q9CEH8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L0350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK05961 ; AAK05961 ; L0350 .
    GeneIDi 1115528.
    KEGGi lla:L0350.
    PATRICi 22296182. VBILacLac136773_1996.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LLAC272623:GHSH-1970-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiSYI_LACLA
    AccessioniPrimary (citable) accession number: Q9CEH8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3