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Q9CEH8 (SYI_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:LL1863
ORF Names:L0350
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length932 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 932932Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098404

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif593 – 5975"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8891Zinc By similarity
Metal binding8921Zinc By similarity
Metal binding9111Zinc By similarity
Metal binding9141Zinc By similarity
Binding site5521Aminoacyl-adenylate By similarity
Binding site5961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CEH8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 85EF03E46A85D751

FASTA932106,509
        10         20         30         40         50         60 
MKIKDTLNLG KTAFPMRAGL PNKEPNWQKD WADATLYEKR QELNEGKPSF MLHDGPPYAN 

        70         80         90        100        110        120 
GNIHLGHSLN KISKDIIVRY KSMAGFRAPY VPGWDTHGLP IEQQLAKAGM KRKEMDLLDY 

       130        140        150        160        170        180 
LEECRKYAMK QVDMQRSDFK SLGVLADWDR PYLTLLPEYE AAQIRVFGKM AEKGYIYKGQ 

       190        200        210        220        230        240 
KPIYWSPSSE SSLAEAEIEY QDVRSASIFV AFKAKDTKGK LPEDVEFVIW TTTPWTIPSN 

       250        260        270        280        290        300 
LGIFAHPDYD YSVVAVNGRK FVIASEMLEA VAEKLEWENP EVLQTIKGSE LEYMVAKHPF 

       310        320        330        340        350        360 
YDRETLIMNA DYVTLDSGTG LVHVAPGHGE DDYFASRKYK LPVLSPIDNR GYYTDEAPGL 

       370        380        390        400        410        420 
EGLLYDEGNK VVSKWLEEKD ALLKLEFFTH SYPHDWRTKK PVIFRATPQW FASIDDFRQN 

       430        440        450        460        470        480 
ILDEVERVDW VIPWGKTRLF NMVRDRGDWV ISRQRAWGVP LPIFYGENGE PIITPETTEH 

       490        500        510        520        530        540 
VAKLFAEFGS KVWFEREAKD LLPEGFTHPA SPNGEFTKEK DIMDVWFDSG SSWNGVLNER 

       550        560        570        580        590        600 
DYLSFPADLY LEGSDQYRGW FNSSITTSVA VNGVAPYKAV LSQGFVLDGK GRKMSKSIGN 

       610        620        630        640        650        660 
TIVPKDVTKK FGADILRLWV ASIDTESDVR VSMDILSQVS EVYRKIRNTL RFLIANTSDF 

       670        680        690        700        710        720 
NPKEDAIDFA ELRPVDKYML VKFNELVKQI RTAYDNYSFM TVYKSIINFI TNDLSSFYLD 

       730        740        750        760        770        780 
FAKDVVYIEA ANSPERRSMQ TVMYVILKDL VKILVPILPH TAEETWTYLE HEPENFAYLA 

       790        800        810        820        830        840 
EMPEAAEIPG SEELLGNWQE FLDFRDKILK ALESAREAKL IGKSLEATVT IYPNEVVRTL 

       850        860        870        880        890        900 
LTAIDENVAQ LLIVSNFVVA NEPVNNAPES AMKFDDLAVL VEHAAGEVCD RCRRTDETVG 

       910        920        930 
HNANEHLKML CEHCAHIVET EFPEILEEGF ED 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005176 Genomic DNA. Translation: AAK05961.1.
PIRG86857.
RefSeqNP_268020.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CEH8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05961; AAK05961; L0350.
GeneID1115528.
KEGGlla:L0350.
PATRIC22296182. VBILacLac136773_1996.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1970-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_LACLA
AccessionPrimary (citable) accession number: Q9CEH8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries