Q9CEF5 (PKNB_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase PknB
EC=2.7.11.1

Gene names

Name:	pknB
Ordered Locus Names:	LL1887
ORF Names:	L138452

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 3 PASTA domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	Probable serine/threonine-protein kinase PknB		PRO_0000171200
Regions
	Domain	12 – 275	264	Protein kinase
	Domain	361 – 428	68	PASTA 1
	Domain	430 – 502	73	PASTA 2
	Domain	503 – 574	72	PASTA 3
	Nucleotide binding	18 – 26	9	ATP By similarity
	Compositional bias	579 – 627	49	Ser-rich
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	42	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CEF5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 00B30B68C5C28A81	FASTA	627	67,878
	10 20 30 40 50 60 MIQIGKIFAD RYRIIKEIGR GGMANVYQGE DTFLGDRLVA IKVLRSNFEN DDIAIARFQR 70 80 90 100 110 120 EAFAMAELSH PNIVGISDVG EFESQQYIVM EFVDGMTLKQ YINQNAPLAN DEAIEIITEI 130 140 150 160 170 180 LSAMDMAHSH GIIHRDLKPQ NVLVSSSGTV KVTDFGIAKA LSETSLTQTN TMFGSVHYLS 190 200 210 220 230 240 PEQARGSNAT VQSDIYAIGI ILFELLTGQI PFDGDSAVAI ALKHFQESIP SIINLNPEVP 250 260 270 280 290 300 QALENVVIKA TAKDIKNRYT DVEEMMTDVA TSTSLDRRGE EKLVFNKDHD ETKIMPANLI 310 320 330 340 350 360 NPYDTKPLID KKEDNDSQTD EKAASSEVGN KNKKSKKGLI IGLIILLLVV GGATLAWVVS 370 380 390 400 410 420 TPTNVKIPNV TNSTLSQAKS KIKDAKLKVG TVHKQQSSTI AEGKVIKTDP TSGTTVRSNS 430 440 450 460 470 480 SVDIYVSTGN EDIIKMKDFV GEKIDEAMAT LLKDYGIDES QVTQTSVPSD SYPAGTIIKQ 490 500 510 520 530 540 SPKKGSSFDT KGSEKITFEV SSGKQVEVPD YKPNGQYMTY SQYQAALKAA GFTNITLDPQ 550 560 570 580 590 600 ATTNQQADGY VYSVYPNVGT SVDPTQEIVV TYSVYTAPSS SSTTSESTTT SETSSSTTSS 610 620 TSSSTTSQPS TDNNNSSKES STTSSSS

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK05985.1.
PIR	G86860.
RefSeq	NP_268044.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CEF5.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L138452.
PTM databases
PhosSite	P0810478.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05985; AAK05985; L138452.
GeneID	1115552.
KEGG	lla:L138452.
PATRIC	22296232. VBILacLac136773_2021.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037186.
KO	K08884.
OMA	TGKDPGT.
ProtClustDB	CLSK698007.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-2031-MONOMER.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR005543. PASTA. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF03793. PASTA. 3 hits. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00740. PASTA. 3 hits. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51178. PASTA. 3 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNB_LACLA

Accession

Primary (citable) accession number: Q9CEF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program