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Protein

Glutamine synthetase

Gene

glnA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei63Important for inhibition by glutamineBy similarity1
Metal bindingi133Magnesium 1By similarity1
Metal bindingi135Magnesium 2By similarity1
Binding sitei185ATPBy similarity1
Metal bindingi190Magnesium 2By similarity1
Metal bindingi197Magnesium 2By similarity1
Binding sitei242L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi246Magnesium 1; via pros nitrogenBy similarity1
Binding sitei250ATPBy similarity1
Binding sitei300L-glutamateBy similarity1
Binding sitei306L-glutamateBy similarity1
Binding sitei318ATPBy similarity1
Binding sitei318L-glutamateBy similarity1
Binding sitei323ATPBy similarity1
Metal bindingi335Magnesium 1By similarity1
Binding sitei337L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:LL2200
ORF Names:L0118
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532421 – 446Glutamine synthetaseAdd BLAST446

Proteomic databases

PaxDbiQ9CDL9.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi272623.L0118.

Structurei

3D structure databases

ProteinModelPortaliQ9CDL9.
SMRiQ9CDL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni241 – 242L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiMGFTCYT.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CDL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITAADIRR DVKEKDIKFL RLMFTDILGT LKNVEVPATD EQLDKLFENK
60 70 80 90 100
MMFDGSSIEG FVRINESDMY LYPDLDTWIV FPWGDEYGKV AGVICDVYTP
110 120 130 140 150
EGEPFAGDPR GVLKRNLKSM EKLGFKSFNL GPEPEFFLFK LNENDEPTLE
160 170 180 190 200
VNDKGGYFDL APTDLAGNTR REIVNVLTDL GFEVEASHHE VAIGQHEIDF
210 220 230 240 250
KYANALKACD NIQIFKLVVK TIARKHGLHA TFMAKPVHGI NGSGMHCNMS
260 270 280 290 300
LFTEDGANAF ADPTGDMGLS DVAHSFIAGL LKHAYNFTAI TNPTVNSYKR
310 320 330 340 350
LVPGYEAPVY VAWAGRNRSP LIRVPASRGL STRVELRAVD PTANPYLALA
360 370 380 390 400
VLLAAGLDGV EHKLEAPEAI ESNIYVMTEE ERKAHGITDL PSTLHNAVKA
410 420 430 440
LPEDTIVTEA LGEHVLVNFV EAKRIEWASY AQFVSQWEID NYLELY
Length:446
Mass (Da):49,693
Last modified:June 1, 2001 - v1
Checksum:iC0A953D5341AD5CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK06298.1.
PIRiH86899.
RefSeqiNP_268357.1. NC_002662.1.
WP_010906377.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK06298; AAK06298; L0118.
GeneIDi1115878.
KEGGilla:L0118.
PATRICi22296956. VBILacLac136773_2362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK06298.1.
PIRiH86899.
RefSeqiNP_268357.1. NC_002662.1.
WP_010906377.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ9CDL9.
SMRiQ9CDL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0118.

Proteomic databases

PaxDbiQ9CDL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK06298; AAK06298; L0118.
GeneIDi1115878.
KEGGilla:L0118.
PATRICi22296956. VBILacLac136773_2362.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiMGFTCYT.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_LACLA
AccessioniPrimary (citable) accession number: Q9CDL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: June 1, 2001
Last modified: March 15, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.