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Q9CCZ9 (TRMB_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:ML2622
ORF Names:MLCL622.20c
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence CAB08804.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

tRNA methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171354

Regions

Region208 – 2114Substrate binding By similarity

Sites

Active site1361 By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1131S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1401Substrate By similarity
Binding site1721Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CCZ9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 082DD06A64624D7B

FASTA23025,821
        10         20         30         40         50         60 
MARHLPATAF RKRRSALSHA QRQTWERLWP EIGISAVSQT RSAERLDTGA WFGRSTLVVL 

        70         80         90        100        110        120 
EVGCGSGTAT LAMAQHEPDI DVIAVEVYRR GLAQLLCAID RDQVHNIRMI HGNALYVLQY 

       130        140        150        160        170        180 
LIAPRSLTGV RVFFPDPWPK VRHHKRRFLQ PATVELIADR LLPGGVLHTA TDHPDYAKQI 

       190        200        210        220        230 
AKFGDGEPLL SRADGRTQLP ISTVRPTTKY ETKAQHAGNV VTELIWKKRS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z95398 Genomic DNA. Translation: CAB08804.1. Different initiation.
AL583926 Genomic DNA. Translation: CAC32154.1.
PIRD87237.
RefSeqNP_302680.1. NC_002677.1.

3D structure databases

ProteinModelPortalQ9CCZ9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML2622.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC32154; CAC32154; CAC32154.
GeneID910881.
KEGGmle:ML2622.
PATRIC18060219. VBIMycLep78757_5023.

Organism-specific databases

LepromaML2622.
CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCLE
AccessionPrimary (citable) accession number: Q9CCZ9
Secondary accession number(s): O06083
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways