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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.
L-glutamine + H2O = L-glutamate + NH3.

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81Nucleophile1
Binding sitei113L-glutamine1
Active sitei177Charge relay system1
Active sitei179Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00245.

Protein family/group databases

MEROPSiC26.A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxT (EC:4.3.3.6)
Alternative name(s):
Pdx2
Pyridoxal 5'-phosphate synthase glutaminase subunit (EC:3.5.1.2)
Gene namesi
Name:pdxT
Ordered Locus Names:ML0474
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0474.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356471 – 198Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST198

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.

Protein-protein interaction databases

STRINGi272631.ML0474.

Structurei

3D structure databases

ProteinModelPortaliQ9CCT5.
SMRiQ9CCT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 51L-glutamine binding3
Regioni141 – 142L-glutamine binding2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX. Bacteria.
COG0311. LUCA.
HOGENOMiHOG000039949.
KOiK08681.
OrthoDBiPOG091H084H.

Family and domain databases

CDDicd01749. GATase1_PB. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01615. PdxT. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR002161. PdxT/SNO.
IPR021196. PdxT/SNO_CS.
PANTHERiPTHR31559. PTHR31559. 1 hit.
PfamiView protein in Pfam
PF01174. SNO. 1 hit.
PIRSFiPIRSF005639. Glut_amidoT_SNO. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR03800. PLP_synth_Pdx2. 1 hit.
PROSITEiView protein in PROSITE
PS01236. PDXT_SNO_1. 1 hit.
PS51130. PDXT_SNO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CCT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFPRVGVLA LQGDTREHLT ALREAGADSM PVRRRGELDE VDALVIPGGE
60 70 80 90 100
STTISHLLLD CELLEPLRAR LADGLPAYGA CTGMILLASE ILDAGVCGRE
110 120 130 140 150
ALPLGAIDIT VRRNAFGRQV DSFEGDIGFA GLVDPVRAVF IRAPWVERAG
160 170 180 190
DGVQVLAQAA GHAVAVRQGS MLATAFHPEM TSDRRIHQLF VDIVNGIA
Length:198
Mass (Da):21,075
Last modified:July 5, 2005 - v2
Checksum:iECF84E45826802CD
GO

Sequence cautioni

The sequence AAA17085 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC29982 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00011 Genomic DNA. Translation: AAA17085.1. Different initiation.
AL583918 Genomic DNA. Translation: CAC29982.1. Different initiation.
PIRiB86968.
S72721.
RefSeqiNP_301419.2. NC_002677.1.
WP_010907743.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29982; CAC29982; CAC29982.
GeneIDi909221.
KEGGimle:ML0474.
PATRICifig|272631.5.peg.835.

Similar proteinsi

Entry informationi

Entry nameiPDXT_MYCLE
AccessioniPrimary (citable) accession number: Q9CCT5
Secondary accession number(s): Q49637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 22, 2017
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families