Q9CCS5 (AROK_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	ML0517

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP-Rule MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP-Rule MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP-Rule MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Shikimate kinase HAMAP-Rule MF_00109		PRO_0000192392
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
Sites
	Metal binding	16	1	Magnesium By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	80	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	136	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CCS5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FCA4D829CB520477	FASTA	199	21,084
	10 20 30 40 50 60 MAPKAVLVGL PGAGKSTIGR RLSKALGVSL LDTDAAIEKQ TGRSIADIFA IDGEEEFRRI 70 80 90 100 110 120 EEGVVRAALV EHDGVVSLGG GAVTSPGVCA ALAGHIVIYL EINAEEAMRR ACGSTVRPLL 130 140 150 160 170 180 AGPDRAEKFQ DLMARRVPLY RRVATIRVDT NCHNLGAVVR YIMARLQAQL ATPVSGGDRK 190 SSEAERSGAP LRKSSEVVK

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583918 Genomic DNA. Translation: CAC30025.1.
PIR	E86973.
RefSeq	NP_301442.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q9CCS5.
SMR	Q9CCS5. Positions 2-167.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML0517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC30025; CAC30025; CAC30025.
GeneID	909274.
KEGG	mle:ML0517.
PATRIC	18051918. VBIMycLep78757_0906.
Organism-specific databases
Leproma	ML0517.
CMR	Search...
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HOG000032568.
KO	K00891.
OMA	ERFQIVE.
ProtClustDB	PRK00131.
Enzyme and pathway databases
UniPathway	UPA00053; UER00088.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase.
InterPro	IPR000623. Shikimate_kinase. IPR023000. Shikimate_kinase_CS. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_MYCLE

Accession

Primary (citable) accession number: Q9CCS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program