Q9CCS3 (AROQ_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type II DHQase

Gene names

Name:	aroQ
Synonyms:	aroD
Ordered Locus Names:	ML0519

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate By similarity. HAMAP MF_00169
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00169
Subunit structure	Homododecamer By similarity. HAMAP MF_00169
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	3-dehydroquinate dehydratase HAMAP MF_00169		PRO_0000159908
Regions
	Region	101 – 102	2	Substrate binding By similarity
Sites
	Active site	23	1	Proton acceptor By similarity
	Active site	100	1	Proton donor By similarity
	Binding site	74	1	Substrate By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Site	18	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CCS3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5FEE5671595F8C0D	FASTA	145	15,643
	10 20 30 40 50 60 MTMVNIINGP NLGRLGRREP DVYGSTTHEQ LSALIERAAV EFGIKAVVRQ SDSESQLLDW 70 80 90 100 110 120 IHLAADAGEP VILNAGGLTH TSVALRDACA ELSAPFIEVH ISNVHAREEF RRHSYLSPLA 130 140 TGVITGLGVQ GYLLALRYLA EIAGN

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583918 Genomic DNA. Translation: CAC30027.1.
PIR	G86973.
RefSeq	NP_301444.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q9CCS3.
SMR	Q9CCS3. Positions 3-141.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029288; EBMYCP00000028894; EBMYCG00000029283.
GeneID	909276.
GenomeReviews	Gene locus ML0519 in contig AL450380_GR.
KEGG	mle:ML0519.
NMPDR	fig|272631.1.peg.316.
PATRIC	18051922. VBIMycLep78757_0908.
Organism-specific databases
Leproma	ML0519.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017464.
HOGENOM	HBG284657.
OMA	GWLHEAA.
ProtClustDB	PRK05395.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0519-MONOMER.
Family and domain databases
HAMAP	MF_00169. AroQ. [Tree]
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
KO	K03786.
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52304. DHquinase_II. 1 hit.
TIGRFAMs	TIGR01088. AroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROQ_MYCLE

Accession

Primary (citable) accession number: Q9CCS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program