Q9CCI3 (AROA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	ML0792

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088272

Sequences

	Sequence		Length	Mass (Da)
	Q9CCI3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 445975BA53DC74D9	FASTA	430	44,558
	10 20 30 40 50 60 MSIESWTAPV ASTPVQANVT IPGSKSQTNR ALMLAALAAA QGQGTSTIGG ALRSRDTELM 70 80 90 100 110 120 ISALRTLGLH VDEAGSVLTV NGRITPGEDA QVDCGLAGTV LRFVPPLAAL SANPVTFDGD 130 140 150 160 170 180 EQARARPITP LLDALRGLGV PVDGVGLPFQ VQGSGSVAGG TVAIDASASS QFVSGLLLCA 190 200 210 220 230 240 ASFTQGVTVQ HTGSPVPSAP HIAMTVMMLR QAGVQVDDSV GNRWQVRPGT VAARHWVVEP 250 260 270 280 290 300 DLTNAVAFLA AAAVSGGTVR ITGWPKTSVQ PADNILNILF RLNVVVNQTD SFLEVQGSTV 310 320 330 340 350 360 YDGFDVDLRD VGELTPSVAA LAALATPGSV SQLHGIAHLR GHETDRLAAL STEINRLGGD 370 380 390 400 410 420 CQETSDGLII TATPLRPGVW RAYADHRMAM AGAIVGLRVS GVEVDDIGAT HKTLPQFPQL 430 WANMLKRSTG

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583919 Genomic DNA. Translation: CAC30301.1.
PIR	A87008.
RefSeq	NP_301607.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q9CCI3.
SMR	Q9CCI3. Positions 3-425.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028666; EBMYCP00000028272; EBMYCG00000028661.
GeneID	909741.
GenomeReviews	Gene locus ML0792 in contig AL450380_GR.
KEGG	mle:ML0792.
NMPDR	fig|272631.1.peg.479.
PATRIC	18052958. VBIMycLep78757_1425.
Organism-specific databases
Leproma	ML0792.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016231.
HOGENOM	HBG646626.
OMA	HRMATAG.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0792-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_MYCLE

Accession

Primary (citable) accession number: Q9CCI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program