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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301Shikimate-3-phosphateUniRule annotation
Binding sitei126 – 1261PhosphoenolpyruvateUniRule annotation
Binding sitei198 – 1981Shikimate-3-phosphateUniRule annotation
Active sitei313 – 3131Proton acceptorUniRule annotation
Active sitei343 – 3431Proton donorUniRule annotation
Binding sitei346 – 3461PhosphoenolpyruvateUniRule annotation
Binding sitei387 – 3871PhosphoenolpyruvateUniRule annotation
Binding sitei412 – 4121PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:ML0792
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0792.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4304303-phosphoshikimate 1-carboxyvinyltransferasePRO_0000088272Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0792.

Structurei

3D structure databases

ProteinModelPortaliQ9CCI3.
SMRiQ9CCI3. Positions 3-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 262Shikimate-3-phosphate bindingUniRule annotation
Regioni96 – 994PhosphoenolpyruvateUniRule annotation
Regioni169 – 1713Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247373.
KOiK00800.
OMAiKSYPDFW.
OrthoDBiPOG091H02JK.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CCI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIESWTAPV ASTPVQANVT IPGSKSQTNR ALMLAALAAA QGQGTSTIGG
60 70 80 90 100
ALRSRDTELM ISALRTLGLH VDEAGSVLTV NGRITPGEDA QVDCGLAGTV
110 120 130 140 150
LRFVPPLAAL SANPVTFDGD EQARARPITP LLDALRGLGV PVDGVGLPFQ
160 170 180 190 200
VQGSGSVAGG TVAIDASASS QFVSGLLLCA ASFTQGVTVQ HTGSPVPSAP
210 220 230 240 250
HIAMTVMMLR QAGVQVDDSV GNRWQVRPGT VAARHWVVEP DLTNAVAFLA
260 270 280 290 300
AAAVSGGTVR ITGWPKTSVQ PADNILNILF RLNVVVNQTD SFLEVQGSTV
310 320 330 340 350
YDGFDVDLRD VGELTPSVAA LAALATPGSV SQLHGIAHLR GHETDRLAAL
360 370 380 390 400
STEINRLGGD CQETSDGLII TATPLRPGVW RAYADHRMAM AGAIVGLRVS
410 420 430
GVEVDDIGAT HKTLPQFPQL WANMLKRSTG
Length:430
Mass (Da):44,558
Last modified:June 1, 2001 - v1
Checksum:i445975BA53DC74D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583919 Genomic DNA. Translation: CAC30301.1.
PIRiA87008.
RefSeqiNP_301607.1. NC_002677.1.
WP_010907931.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30301; CAC30301; CAC30301.
GeneIDi909741.
KEGGimle:ML0792.
PATRICi18052958. VBIMycLep78757_1425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583919 Genomic DNA. Translation: CAC30301.1.
PIRiA87008.
RefSeqiNP_301607.1. NC_002677.1.
WP_010907931.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ9CCI3.
SMRiQ9CCI3. Positions 3-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30301; CAC30301; CAC30301.
GeneIDi909741.
KEGGimle:ML0792.
PATRICi18052958. VBIMycLep78757_1425.

Organism-specific databases

LepromaiML0792.

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247373.
KOiK00800.
OMAiKSYPDFW.
OrthoDBiPOG091H02JK.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROA_MYCLE
AccessioniPrimary (citable) accession number: Q9CCI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.