Q9CBY3 (CLPP1_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 1
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 1

Gene names

Name:	clpP1
Synonyms:	clpP
Ordered Locus Names:	ML1480

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.
Sequence caution	The sequence CAC30430.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179592
Sites
	Active site	98	1	By similarity
	Active site	123	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CBY3 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: E1B0AE6C9AF83EE4	FASTA	200	21,847
	10 20 30 40 50 60 MGHVTDIRSN SQGFNLTDSV YERLLSERII FLGSEVNDNI ANRLCAQILL LAAEDAFKDI 70 80 90 100 110 120 SLYINSPGGS ISAGMAIYDT MVLAPCDIAT YAMGMAASMG EFLLAAGTRG KRYALPHARI 130 140 150 160 170 180 LMHQPLGGVT GSAADIAIQA EQFAVIKKEM FRLNAEFTGQ PIERIEADSD RDRWFTASEA 190 200 LEYGFVDHII TRAHVNGEVQ

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583922 Genomic DNA. Translation: CAC30430.1. Different initiation.
PIR	A87094.
RefSeq	NP_302041.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q9CBY3.
SMR	Q9CBY3. Positions 15-194.
ModBase	Search...
Protein family/group databases
MEROPS	S14.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028743; EBMYCP00000028349; EBMYCG00000028738.
GeneID	909508.
GenomeReviews	Gene locus ML1480 in contig AL450380_GR.
KEGG	mle:ML1480.
NMPDR	fig|272631.1.peg.913.
PATRIC	18055678. VBIMycLep78757_2772.
Organism-specific databases
Leproma	ML1480.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015874.
HOGENOM	HBG558421.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1480-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_MYCLE

Accession

Primary (citable) accession number: Q9CBY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	September 26, 2001
Last modified:	January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program