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Protein

FO synthase

Gene

fbiC

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO).By similarity

Catalytic activityi

5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. The clusters are coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi: coenzyme F0 biosynthesis

This protein is involved in the pathway coenzyme F0 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F0 biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi105 – 1051Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi109 – 1091Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi112 – 1121Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi565 – 5651Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi569 – 5691Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi572 – 5721Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00072.

Names & Taxonomyi

Protein namesi
Recommended name:
FO synthase (EC:2.5.1.77)
Alternative name(s):
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
Gene namesi
Name:fbiC
Ordered Locus Names:ML1492
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1492.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863FO synthasePRO_0000147770Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1492.

Structurei

3D structure databases

ProteinModelPortaliQ9CBX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. CofG family.Curated
In the C-terminal section; belongs to the radical SAM superfamily. CofH family.Curated

Phylogenomic databases

eggNOGiENOG4105DKF. Bacteria.
COG1060. LUCA.
HOGENOMiHOG000020613.
KOiK11779.
OMAiHWVGHLN.
OrthoDBiEOG6CCH3R.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_01611. FO_synth_sub1.
MF_01612. FO_synth_sub2.
InterProiIPR013785. Aldolase_TIM.
IPR019939. CofG_family.
IPR019940. CofH_family.
IPR006638. Elp3/MiaB/NifB.
IPR020050. FO_synthase_su2.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 2 hits.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03550. F420_cofG. 1 hit.
TIGR03551. F420_cofH. 1 hit.
TIGR00423. TIGR00423. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CBX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGSYTKVSL IESQEPIALS RPVVPPKPNT SALRRVLRRA RDGFALNIDE
60 70 80 90 100
AVVAMTARGE DLADLCASAA RVRDVGLETA GRRGADGRLP ITYSRKVFIP
110 120 130 140 150
VTHLCRDSCH YCTFVTAPDM LRTQGAGMYL EPNEILNLAR RGSELGCKEA
160 170 180 190 200
LFTLGDRPED RWAQARDWLA ERGYDSTLSY LRAMAIRVLE ETGLLPHLNP
210 220 230 240 250
GVMSWSELSR LKPVAPSMGM MLETTSRRLF ETKGLAHYGS LDKDPTVRLR
260 270 280 290 300
VLTDAGRLSI PFTTGLLVGI GETLAERADT LHEIRKSNKE FGHVQEVIVQ
310 320 330 340 350
NFRAKEHTAM AAVPDARIED YLATVAVARL VLGPAMRIQA PPNLVSREEC
360 370 380 390 400
LALVTAGVDD WGGVSPLTPD HVNPERPWPA LHELAAVTAE AGYTLVQRLT
410 420 430 440 450
AQPKYVQAGA AWIDPRVRGH VVALADPVTG LARDVNPVGM PWQEPDDVES
460 470 480 490 500
AGRMDINTAI DTEGRNTEAR SDLDSAFGDW ESIRAHVHEL ADCAPERIDT
510 520 530 540 550
DVLAALRSAE RDPAGCTDDE YLALATADGP ALEAVTALAD SLRRDVVGDD
560 570 580 590 600
VTFVVNRNIN FTNICYTGCR FCAFAQRKGD TDAYSLSREE VAERAWEAHV
610 620 630 640 650
QGATEVCMQG GIDPELPVTG YVDLVRAVKT RVPSMHVHAF SPMEIANGVA
660 670 680 690 700
KSGFSIREWL ISLREAGLDT IPGTAAEILD DEVRWVLTKG KLPTSMWIEI
710 720 730 740 750
VTTAHEVGLR SSSTMMYGHV DGPRHWVAHL QVLRDIQDRT GGFTEFVPLP
760 770 780 790 800
FVHQNSPLYL AGAARPGPTH RDNRAVHALA RIMLHGRISH IQTSWVKLGV
810 820 830 840 850
ERTQAMLNGG ANDLGGTLME ETISRMAGSE YGSAKTVAEL IAIAEGIGRT
860
ARQRTTTYAL RGA
Length:863
Mass (Da):94,360
Last modified:June 1, 2001 - v1
Checksum:i7DFD519DBC19EF86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583922 Genomic DNA. Translation: CAC30442.1.
PIRiE87095.
RefSeqiNP_302048.1. NC_002677.1.
WP_010908369.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30442; CAC30442; CAC30442.
GeneIDi909524.
KEGGimle:ML1492.
PATRICi18055738. VBIMycLep78757_2800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583922 Genomic DNA. Translation: CAC30442.1.
PIRiE87095.
RefSeqiNP_302048.1. NC_002677.1.
WP_010908369.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ9CBX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30442; CAC30442; CAC30442.
GeneIDi909524.
KEGGimle:ML1492.
PATRICi18055738. VBIMycLep78757_2800.

Organism-specific databases

LepromaiML1492.

Phylogenomic databases

eggNOGiENOG4105DKF. Bacteria.
COG1060. LUCA.
HOGENOMiHOG000020613.
KOiK11779.
OMAiHWVGHLN.
OrthoDBiEOG6CCH3R.

Enzyme and pathway databases

UniPathwayiUPA00072.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_01611. FO_synth_sub1.
MF_01612. FO_synth_sub2.
InterProiIPR013785. Aldolase_TIM.
IPR019939. CofG_family.
IPR019940. CofH_family.
IPR006638. Elp3/MiaB/NifB.
IPR020050. FO_synthase_su2.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 2 hits.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03550. F420_cofG. 1 hit.
TIGR03551. F420_cofH. 1 hit.
TIGR00423. TIGR00423. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiFBIC_MYCLE
AccessioniPrimary (citable) accession number: Q9CBX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: November 11, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.