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Protein

FO synthase

Gene

fbiC

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO).By similarity

Catalytic activityi

5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. The clusters are coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi: coenzyme F0 biosynthesis

This protein is involved in the pathway coenzyme F0 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F0 biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi109Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi112Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi565Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi569Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi572Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-1636-MONOMER
UniPathwayiUPA00072

Names & Taxonomyi

Protein namesi
Recommended name:
FO synthase (EC:2.5.1.77)
Alternative name(s):
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
Gene namesi
Name:fbiC
Ordered Locus Names:ML1492
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi

Organism-specific databases

LepromaiML1492

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001477701 – 863FO synthaseAdd BLAST863

Proteomic databases

PRIDEiQ9CBX6

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1492

Structurei

3D structure databases

ProteinModelPortaliQ9CBX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. CofG family.Curated
In the C-terminal section; belongs to the radical SAM superfamily. CofH family.Curated

Phylogenomic databases

eggNOGiENOG4105DKF Bacteria
COG1060 LUCA
HOGENOMiHOG000020613
KOiK11779
OMAiHWVGHLN
OrthoDBiPOG091H03LO

Family and domain databases

Gene3Di3.20.20.70, 2 hits
HAMAPiMF_01611 FO_synth_sub1, 1 hit
MF_01612 FO_synth_sub2, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR019939 CofG_family
IPR019940 CofH_family
IPR006638 Elp3/MiaB/NifB
IPR034405 F420
IPR020050 FO_synthase_su2
IPR007197 rSAM
PANTHERiPTHR43076 PTHR43076, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 2 hits
SFLDiSFLDF00293 ((2_3_4_5-tetrahydroxypentyl)a, 1 hit
SFLDF00294 7_8-didemethyl-8-hydroxy-5-dea, 1 hit
SFLDG01064 F420__menaquinone_cofactor_bio, 2 hits
SFLDG01389 menaquinone_synthsis_involved, 1 hit
SFLDS00029 Radical_SAM, 2 hits
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR03550 F420_cofG, 1 hit
TIGR03551 F420_cofH, 1 hit
TIGR00423 TIGR00423, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CBX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGSYTKVSL IESQEPIALS RPVVPPKPNT SALRRVLRRA RDGFALNIDE
60 70 80 90 100
AVVAMTARGE DLADLCASAA RVRDVGLETA GRRGADGRLP ITYSRKVFIP
110 120 130 140 150
VTHLCRDSCH YCTFVTAPDM LRTQGAGMYL EPNEILNLAR RGSELGCKEA
160 170 180 190 200
LFTLGDRPED RWAQARDWLA ERGYDSTLSY LRAMAIRVLE ETGLLPHLNP
210 220 230 240 250
GVMSWSELSR LKPVAPSMGM MLETTSRRLF ETKGLAHYGS LDKDPTVRLR
260 270 280 290 300
VLTDAGRLSI PFTTGLLVGI GETLAERADT LHEIRKSNKE FGHVQEVIVQ
310 320 330 340 350
NFRAKEHTAM AAVPDARIED YLATVAVARL VLGPAMRIQA PPNLVSREEC
360 370 380 390 400
LALVTAGVDD WGGVSPLTPD HVNPERPWPA LHELAAVTAE AGYTLVQRLT
410 420 430 440 450
AQPKYVQAGA AWIDPRVRGH VVALADPVTG LARDVNPVGM PWQEPDDVES
460 470 480 490 500
AGRMDINTAI DTEGRNTEAR SDLDSAFGDW ESIRAHVHEL ADCAPERIDT
510 520 530 540 550
DVLAALRSAE RDPAGCTDDE YLALATADGP ALEAVTALAD SLRRDVVGDD
560 570 580 590 600
VTFVVNRNIN FTNICYTGCR FCAFAQRKGD TDAYSLSREE VAERAWEAHV
610 620 630 640 650
QGATEVCMQG GIDPELPVTG YVDLVRAVKT RVPSMHVHAF SPMEIANGVA
660 670 680 690 700
KSGFSIREWL ISLREAGLDT IPGTAAEILD DEVRWVLTKG KLPTSMWIEI
710 720 730 740 750
VTTAHEVGLR SSSTMMYGHV DGPRHWVAHL QVLRDIQDRT GGFTEFVPLP
760 770 780 790 800
FVHQNSPLYL AGAARPGPTH RDNRAVHALA RIMLHGRISH IQTSWVKLGV
810 820 830 840 850
ERTQAMLNGG ANDLGGTLME ETISRMAGSE YGSAKTVAEL IAIAEGIGRT
860
ARQRTTTYAL RGA
Length:863
Mass (Da):94,360
Last modified:June 1, 2001 - v1
Checksum:i7DFD519DBC19EF86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583922 Genomic DNA Translation: CAC30442.1
PIRiE87095
RefSeqiNP_302048.1, NC_002677.1
WP_010908369.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC30442; CAC30442; CAC30442
GeneIDi909524
KEGGimle:ML1492
PATRICifig|272631.5.peg.2800

Similar proteinsi

Entry informationi

Entry nameiFBIC_MYCLE
AccessioniPrimary (citable) accession number: Q9CBX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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