Reviewed, UniProtKB/Swiss-Prot Q9CBW1 (DYR_MYCLE)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:	folA
Ordered Locus Names:	ML1518

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Dihydrofolate reductase		PRO_0000186399
Regions
	Domain	3 – 165	163	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q9CBW1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E6A76965BFBEC74F	FASTA	165	18,509
	10 20 30 40 50 60 MTVVGLIWAQ STTGVIGRDG GMPWRVPEDL TRFKQLTMGH PVVMGRRTWD SLPVGVRPLP 70 80 90 100 110 120 GRRNIVLSRQ IDFMAEGAEV FGSLEETISN LETEPEMWVI GGEQIYRLAL PLATRCGVTE 130 140 150 160 VDTYLLHEDD DVLAPVLDDT WVGVTGEWLV SCSGLRYRLH SYHRS

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL583922 Genomic DNA. Translation: CAC30469.1.
PIR	H87098.
RefSeq	NP_302063.1.
3D structure databases
HSSP	HSSP built from PDB template 1DF7 based on UniProtKB O33305.
SMR	Q9CBW1. Positions 3-165.
ModBase	Search...
Genome annotation databases
GeneID	909551.
GenomeReviews	Gene locus ML1518 in contig AL450380_GR.
KEGG	mle:ML1518.
NMPDR	fig|272631.1.peg.935.
Organism-specific databases
Leproma	ML1518.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CBW1.
OMA	Q9CBW1. WVIGGER.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1518-MON.
BRENDA	1.5.1.3. 808.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. IPR017925. Dihydrofolate_reductase_CS. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_MYCLE

Accession

Primary (citable) accession number: Q9CBW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)