Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:ML1630
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1630.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092551 – 1004Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST1004

Proteomic databases

PRIDEiQ9CBT4.

Interactioni

Protein-protein interaction databases

STRINGi272631.ML1630.

Structurei

3D structure databases

ProteinModelPortaliQ9CBT4.
SMRiQ9CBT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 497Adenylyl removaseUniRule annotationAdd BLAST497
Regioni502 – 1004Adenylyl transferaseUniRule annotationAdd BLAST503

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9CBT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCTTVVVVR KPATERLKRP SVGRLGLFDP HAAENLALLG WYDYDCREHV
60 70 80 90 100
DLLWSLSRAP DADAVLRPMV RLFENPATGW DELNAALLTD RGLRGRLFAV
110 120 130 140 150
LGSSLALGDH LVAHPQSWKL LRGRVSLPSR TQLHQAFDEC VSNFGDDPLD
160 170 180 190 200
APDSVLPRLR TLYCDQLLVL AALDLAATVE DEPVLPFTLV AAQLADIADA
210 220 230 240 250
AMATALRVAE KIVCGDRTPP RLAVIAMGKW GARELNYVSD VDIIFVAEQA
260 270 280 290 300
DPLSTRVASE MMRVASEAFF QVDAGLRPEG RNGELVRTVE SHIAYYQRWA
310 320 330 340 350
KTWEFQALLK ARSAVGDAEL GERYLAALMP MVWAACERAD FVAEVQAMRR
360 370 380 390 400
RVEQLVPADV RGRELKLGSG GLRDVEFAVQ LLQLVHGRSD ESLHVVSTVD
410 420 430 440 450
ALAALGEGGY IGREDAANLT ASYEFLRLLE HRLQLQRLKR THLLPEDGDE
460 470 480 490 500
EAVRWLARAA HIRPDGRHDA AGVLREELKN QNLRVSQLHA KLFYQPLLES
510 520 530 540 550
IGPASLEIRH GMTSEAAERQ LATLGYEGSQ TALKHISALV NQSGRRGRVQ
560 570 580 590 600
SVLLPRLLNW MSYAPDPDGG LLAYRRLSEA LSAETWYLST LRDKPAVARR
610 620 630 640 650
LMHVLGTSVY VPDLLMRAPE VIQSYGDGLA GPKLLEAEPA MVARALITSA
660 670 680 690 700
GRHTDPIRAI DAARSLRRRE LARVGSADLL GLLEVTEVCK ALTSVWVAVL
710 720 730 740 750
QAALDAVIRA YLPDGDKAPA AIAVIGMGRL GGAELGYGSD ADVMFVCEPA
760 770 780 790 800
IGVEDAQALR WSGMIAERVC RLLRTPSVDP PLDVDANLRP EGRNGPLVRT
810 820 830 840 850
LGAYAAYYAQ WAQPWEIQAL LRAHAVAGDA DLGQRFLLMA DKMRYPPDGV
860 870 880 890 900
SAEAVREIRR MKARVEAERL PRGADPHTHT KLGRGGLADI EWTVQLMQLR
910 920 930 940 950
HAHELPALHK TSTLESLDAI AAANLIPEAD VDLLRQAWLT ATRARNALVL
960 970 980 990 1000
VRGKTTDQLP GPGRQLNAVA VAAGWPSDDG SEFLDNYLRV TRRAKTFVRK

VFGS
Length:1,004
Mass (Da):110,238
Last modified:June 1, 2001 - v1
Checksum:iDAE22EE45EB5012C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583922 Genomic DNA. Translation: CAC30581.1.
PIRiH87112.
RefSeqiNP_302122.1. NC_002677.1.
WP_010908443.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30581; CAC30581; CAC30581.
GeneIDi909837.
KEGGimle:ML1630.
PATRICi18056282. VBIMycLep78757_3073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583922 Genomic DNA. Translation: CAC30581.1.
PIRiH87112.
RefSeqiNP_302122.1. NC_002677.1.
WP_010908443.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ9CBT4.
SMRiQ9CBT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML1630.

Proteomic databases

PRIDEiQ9CBT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30581; CAC30581; CAC30581.
GeneIDi909837.
KEGGimle:ML1630.
PATRICi18056282. VBIMycLep78757_3073.

Organism-specific databases

LepromaiML1630.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_MYCLE
AccessioniPrimary (citable) accession number: Q9CBT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.