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Q9CBQ0

- RIR1_MYCLE

UniProt

Q9CBQ0 - RIR1_MYCLE

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene
nrdE, ML1734
Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681Substrate By similarity
Sitei185 – 1851Important for hydrogen atom transfer By similarity
Sitei192 – 1921Allosteric effector binding By similarity
Binding sitei213 – 2131Substrate; via amide nitrogen By similarity
Sitei222 – 2221Allosteric effector binding By similarity
Active sitei393 – 3931Proton acceptor By similarity
Active sitei395 – 3951Cysteine radical intermediate By similarity
Active sitei397 – 3971Proton acceptor By similarity
Sitei422 – 4221Important for hydrogen atom transfer By similarity
Sitei699 – 6991Important for electron transfer By similarity
Sitei700 – 7001Important for electron transfer By similarity
Sitei716 – 7161Interacts with thioredoxin/glutaredoxin By similarity
Sitei719 – 7191Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R1 subunit
Gene namesi
Name:nrdE
Ordered Locus Names:ML1734
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML1734.

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi185 ↔ 422Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi272631.ML1734.

Structurei

3D structure databases

ProteinModelPortaliQ9CBQ0.
SMRiQ9CBQ0. Positions 16-706.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1852Substrate binding By similarity
Regioni393 – 3975Substrate binding By similarity
Regioni595 – 5995Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000246165.
KOiK00525.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CBQ0-1 [UniParc]FASTAAdd to Basket

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MPPTATAEPV TASTHMLSNE TDYHALNAML NLYDADGKIQ FDKDVLAARQ    50
FFLQHVNQNT VFFHNQDEKL DYLIRENYYE REVLDQYSRN FVKSLLDRAY 100
AKKFRFSTFL GAFKYYTSYT LKTFDGKRYL ERLEDRVCMV ALTLAAGDTG 150
LAEKLVDEII DGRFQPATPT FLNSGKKQRG EPVSCFLLRI EDNMESIGRS 200
VNSALQLSKR GGGVALLLSN IREHGAPIKN IEHQSSGVIP IMKLLEDAFS 250
YANQLGARQG AGAVYLHAHH PDIYRFLDTK RENADEKIRI KTLSLGVIIP 300
DITFELAKRN EDMYLFSSYD VERVYEVPFA DISVTEKYYE MLDDARIRKT 350
KIKAREFFQK LAELQFESGY PYVMFEDTVN RANPIEGKIT HSNLCSEILQ 400
VSTPSLFNDD LSYAKVGKDI SCNLGSLNIA KTMDSPDFAQ TVEVAIRALT 450
AVSDQTHIKS VPSIEQGNND SHAIGLGQMN LHGYLSREGI FYGSEEGVDF 500
TNIYFYTVLF HVLLASNSIA IERGTFFKGF ERSKYASGEF FDKYIEQTWE 550
PKTDKVRQLF AEAAIRIPTQ NDWKRLKELV VAHGIYNQNL QAVPPTGSIS 600
YINHSTSSIH PIVSKVEIRK EGKIGRVYYP APYMTNDNLQ YYQDAYEIGY 650
QKIIDTYAAA TQHVDQGLSL TLFFKDTATT RDVNKAQIYA WRKGIKTLYY 700
IRLRQMALEG TEVEGCVSCT L 721
Length:721
Mass (Da):82,024
Last modified:August 29, 2001 - v2
Checksum:i22B365A016228872
GO

Sequence cautioni

The sequence CAC30687.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL583923 Genomic DNA. Translation: CAC30687.1. Different initiation.
PIRiH87125.
RefSeqiNP_302195.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30687; CAC30687; CAC30687.
GeneIDi910759.
KEGGimle:ML1734.
PATRICi18056662. VBIMycLep78757_3260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL583923 Genomic DNA. Translation: CAC30687.1 . Different initiation.
PIRi H87125.
RefSeqi NP_302195.1. NC_002677.1.

3D structure databases

ProteinModelPortali Q9CBQ0.
SMRi Q9CBQ0. Positions 16-706.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML1734.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC30687 ; CAC30687 ; CAC30687 .
GeneIDi 910759.
KEGGi mle:ML1734.
PATRICi 18056662. VBIMycLep78757_3260.

Organism-specific databases

Lepromai ML1734.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000246165.
KOi K00525.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiRIR1_MYCLE
AccessioniPrimary (citable) accession number: Q9CBQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: May 14, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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