Ribonucleoside-diphosphate reductase subunit alpha

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase R1 subunit

Gene names

Name:	nrdE
Ordered Locus Names:	ML1734

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

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Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

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Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 721

721

Ribonucleoside-diphosphate reductase subunit alpha

PRO_0000187220

Sites

Active site

185

1

Hydrogen atom transfer By similarity

Active site

393

1

Proton acceptor By similarity

Active site

395

1

Proton acceptor By similarity

Active site

397

1

Proton acceptor By similarity

Active site

422

1

Hydrogen atom transfer By similarity

Active site

699

1

Electron transfer By similarity

Active site

700

1

Electron transfer By similarity

Site

192

1

Allosteric effector binding By similarity

Site

222

1

Allosteric effector binding By similarity

Site

716

1

Interacts with thioredoxin/glutaredoxin By similarity

Site

719

1

Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond

185 ↔ 422

Redox-active By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9CBQ0-1 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 22B365A016228872
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FASTA

721

82,024

        10         20         30         40         50         60 
MPPTATAEPV TASTHMLSNE TDYHALNAML NLYDADGKIQ FDKDVLAARQ FFLQHVNQNT 

        70         80         90        100        110        120 
VFFHNQDEKL DYLIRENYYE REVLDQYSRN FVKSLLDRAY AKKFRFSTFL GAFKYYTSYT 

       130        140        150        160        170        180 
LKTFDGKRYL ERLEDRVCMV ALTLAAGDTG LAEKLVDEII DGRFQPATPT FLNSGKKQRG 

       190        200        210        220        230        240 
EPVSCFLLRI EDNMESIGRS VNSALQLSKR GGGVALLLSN IREHGAPIKN IEHQSSGVIP 

       250        260        270        280        290        300 
IMKLLEDAFS YANQLGARQG AGAVYLHAHH PDIYRFLDTK RENADEKIRI KTLSLGVIIP 

       310        320        330        340        350        360 
DITFELAKRN EDMYLFSSYD VERVYEVPFA DISVTEKYYE MLDDARIRKT KIKAREFFQK 

       370        380        390        400        410        420 
LAELQFESGY PYVMFEDTVN RANPIEGKIT HSNLCSEILQ VSTPSLFNDD LSYAKVGKDI 

       430        440        450        460        470        480 
SCNLGSLNIA KTMDSPDFAQ TVEVAIRALT AVSDQTHIKS VPSIEQGNND SHAIGLGQMN 

       490        500        510        520        530        540 
LHGYLSREGI FYGSEEGVDF TNIYFYTVLF HVLLASNSIA IERGTFFKGF ERSKYASGEF 

       550        560        570        580        590        600 
FDKYIEQTWE PKTDKVRQLF AEAAIRIPTQ NDWKRLKELV VAHGIYNQNL QAVPPTGSIS 

       610        620        630        640        650        660 
YINHSTSSIH PIVSKVEIRK EGKIGRVYYP APYMTNDNLQ YYQDAYEIGY QKIIDTYAAA 

       670        680        690        700        710        720 
TQHVDQGLSL TLFFKDTATT RDVNKAQIYA WRKGIKTLYY IRLRQMALEG TEVEGCVSCT 


L

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References

[1]

"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S.

Barrell B.G.
Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.

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Cross-references

Sequence databases
	AL583923 Genomic DNA. Translation: CAC30687.1. Different initiation.
PIR	H87125.
RefSeq	NP_302195.1.
3D structure databases
SMR	Q9CBQ0. Positions 16-706.
ModBase	Search...
Genome annotation databases
GeneID	910759.
GenomeReviews	Gene locus ML1734 in contig AL450380_GR.
KEGG	mle:ML1734.
NMPDR	fig\|272631.1.peg.1067.
Organism-specific databases
Leproma	ML1734.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CBQ0.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1734-MON.
BRENDA	1.17.4.1. 808.
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. IPR013554. Ribonucl_Rdtase_N. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. PF08343. RNR_N. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

RIR1_MYCLE

Accession

Primary (citable) accession number: Q9CBQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents