Reviewed, UniProtKB/Swiss-Prot Q9CBM9 (CBL_MYCLE)

Names and origin

Protein names

Recommended name:
    Putative cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Ordered Locus Names:

ML1794

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/patB cystathionine beta-lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Putative cystathionine beta-lyase		PRO_0000163849
Amino acid modifications
	Modified residue	236	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CBM9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7696E8684CB220D6	FASTA	402	43,861
	10 20 30 40 50 60 MTSNPLEQLT IEQLQCRTSM KWRAHPEDVL PLWVAEMDVL LAPTVAEAVH RAVDNGDTGY 70 80 90 100 110 120 PYGTTFAEAI SEFASQRWQW HDLDVSRTAI VPDVMLGVVE MLRLITDRGD GVIVSPPVYP 130 140 150 160 170 180 PFYAFVTHDG RRVLEAPLGR DGRLDLAALQ DAFSRARRSS GPNGKVAYLL CNPHNPTGSV 190 200 210 220 230 240 HTVAELHGVA ELARRFGVRV ISDEIHAPLV LSGARFTPYL SIPGAENAFA LMSATKGWNL 250 260 270 280 290 300 CGLKAAIAIA GREAAADLRR IPKEVSHGPS HLGIIAHTAA FRTGSSWLDA LLQGLEANRA 310 320 330 340 350 360 LLRDLVTKHL PGVRYQRPQG TYLTWLDCRH LGFDEQINEG LAAISDLSGP ARWFLDHSRV 370 380 390 400 ALTSGHAFGT GGVGHVRVNF ATSQAILIEA LSRMGRALVN FR

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL583923 Genomic DNA. Translation: CAC30747.1.
PIR	C87133.
RefSeq	NP_302217.1.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ModBase	Search...
Genome annotation databases
GeneID	910697.
GenomeReviews	Gene locus ML1794 in contig AL450380_GR.
KEGG	mle:ML1794.
NMPDR	fig|272631.1.peg.1089.
Organism-specific databases
Leproma	ML1794.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CBM9.
OMA	PEEVSHG.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1794-MON.
Family and domain databases
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PROSITE	PS00599. AA_TRANSFER_CLASS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBL_MYCLE

Accession

Primary (citable) accession number: Q9CBM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)