Q9CBM9 (CBL_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cystathionine beta-lyase
Short name=CBL
EC=4.4.1.8

Alternative name(s):
Beta-cystathionase
Cysteine lyase

Gene names

Ordered Locus Names:

ML1794

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Putative cystathionine beta-lyase		PRO_0000163849
Amino acid modifications
	Modified residue	236	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CBM9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7696E8684CB220D6	FASTA	402	43,861
	10 20 30 40 50 60 MTSNPLEQLT IEQLQCRTSM KWRAHPEDVL PLWVAEMDVL LAPTVAEAVH RAVDNGDTGY 70 80 90 100 110 120 PYGTTFAEAI SEFASQRWQW HDLDVSRTAI VPDVMLGVVE MLRLITDRGD GVIVSPPVYP 130 140 150 160 170 180 PFYAFVTHDG RRVLEAPLGR DGRLDLAALQ DAFSRARRSS GPNGKVAYLL CNPHNPTGSV 190 200 210 220 230 240 HTVAELHGVA ELARRFGVRV ISDEIHAPLV LSGARFTPYL SIPGAENAFA LMSATKGWNL 250 260 270 280 290 300 CGLKAAIAIA GREAAADLRR IPKEVSHGPS HLGIIAHTAA FRTGSSWLDA LLQGLEANRA 310 320 330 340 350 360 LLRDLVTKHL PGVRYQRPQG TYLTWLDCRH LGFDEQINEG LAAISDLSGP ARWFLDHSRV 370 380 390 400 ALTSGHAFGT GGVGHVRVNF ATSQAILIEA LSRMGRALVN FR

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583923 Genomic DNA. Translation: CAC30747.1.
PIR	C87133.
RefSeq	NP_302217.1. NC_002677.1.
3D structure databases
ProteinModelPortal	Q9CBM9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028483; EBMYCP00000028089; EBMYCG00000028478.
GeneID	910697.
GenomeReviews	Gene locus ML1794 in contig AL450380_GR.
KEGG	mle:ML1794.
NMPDR	fig|272631.1.peg.1089.
PATRIC	18056976. VBIMycLep78757_3415.
Organism-specific databases
Leproma	ML1794.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000032189.
HOGENOM	HBG681988.
OMA	WNLAGLK.
ProtClustDB	CLSK872043.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1794-MONOMER.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K14155.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBL_MYCLE

Accession

Primary (citable) accession number: Q9CBM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program