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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei945-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei955-phosphoribose 1-diphosphateUniRule annotation1
Binding sitei985-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei124OrotateUniRule annotation1
Binding sitei152OrotateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:ML2487
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2487.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001107101 – 179Orotate phosphoribosyltransferaseAdd BLAST179

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML2487.

Structurei

3D structure databases

ProteinModelPortaliQ9CB28.
SMRiQ9CB28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 1285-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037976.
KOiK00762.
OMAiYVNCKPV.
OrthoDBiPOG091H034Q.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CB28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELDRKELA DLVRRLSVVQ GCVTLSSGKQ ADHYVDLRRA TLHHRASALI
60 70 80 90 100
GRLMRELTKD WNYTVVGGLT LGADPVAIAI IHSPGRPIDA FVVRKYAKTH
110 120 130 140 150
GLQRLIEGSE VSGQRVLVVE DTSTTGASAL TAVRAVQDAG GDVVGVATVV
160 170
DRGTGAAEAI KAEGLLYRSV LGLADLGLG
Length:179
Mass (Da):18,879
Last modified:June 1, 2001 - v1
Checksum:iDF243F7CCEC8BF3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583925 Genomic DNA. Translation: CAC32004.1.
PIRiD87220.
RefSeqiNP_302606.1. NC_002677.1.
WP_010908925.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC32004; CAC32004; CAC32004.
GeneIDi908475.
KEGGimle:ML2487.
PATRICi18059729. VBIMycLep78757_4779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583925 Genomic DNA. Translation: CAC32004.1.
PIRiD87220.
RefSeqiNP_302606.1. NC_002677.1.
WP_010908925.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ9CB28.
SMRiQ9CB28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC32004; CAC32004; CAC32004.
GeneIDi908475.
KEGGimle:ML2487.
PATRICi18059729. VBIMycLep78757_4779.

Organism-specific databases

LepromaiML2487.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037976.
KOiK00762.
OMAiYVNCKPV.
OrthoDBiPOG091H034Q.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_MYCLE
AccessioniPrimary (citable) accession number: Q9CB28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.