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Q9CAY3 (GPAT5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate acyltransferase 5
Short name=AtGPAT5
EC=2.3.1.15
Gene names
Name:GPAT5
Ordered Locus Names:At3g11430
ORF Names:F24K9.10
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.

Catalytic activity

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.5

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Weakly or not expressed in roots, leaves, seedlings, developing siliques and flower buds. Ref.5

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.

Sequence similarities

Belongs to the GPAT/DAPAT family.

Biophysicochemical properties

Kinetic parameters:

Vmax=86.85 pmol/min/mg enzyme Ref.5

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Glycerol-3-phosphate acyltransferase 5
PRO_0000195253

Regions

Transmembrane31 – 5121Helical; Potential
Transmembrane237 – 25721Helical; Potential
Motif300 – 3056HXXXXD motif

Sequences

Sequence LengthMass (Da)Tools
Q9CAY3 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 32E612BD3A21CD6E

FASTA50256,081
        10         20         30         40         50         60 
MVMEQAGTTS YSVVSEFEGT ILKNADSFSY FMLVAFEAAG LIRFAILLFL WPVITLLDVF 

        70         80         90        100        110        120 
SYKNAALKLK IFVATVGLRE PEIESVARAV LPKFYMDDVS MDTWRVFSSC KKRVVVTRMP 

       130        140        150        160        170        180 
RVMVERFAKE HLRADEVIGT ELIVNRFGFV TGLIRETDVD QSALNRVANL FVGRRPQLGL 

       190        200        210        220        230        240 
GKPALTASTN FLSLCEEHIH APIPENYNHG DQQLQLRPLP VIFHDGRLVK RPTPATALII 

       250        260        270        280        290        300 
LLWIPFGIIL AVIRIFLGAV LPLWATPYVS QIFGGHIIVK GKPPQPPAAG KSGVLFVCTH 

       310        320        330        340        350        360 
RTLMDPVVLS YVLGRSIPAV TYSISRLSEI LSPIPTVRLT RIRDVDAAKI KQQLSKGDLV 

       370        380        390        400        410        420 
VCPEGTTCRE PFLLRFSALF AELTDRIVPV AMNYRVGFFH ATTARGWKGL DPIFFFMNPR 

       430        440        450        460        470        480 
PVYEITFLNQ LPMEATCSSG KSPHDVANYV QRILAATLGF ECTNFTRKDK YRVLAGNDGT 

       490        500 
VSYLSLLDQL KKVVSTFEPC LH

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility."
Zheng Z., Xia Q., Dauk M., Shen W., Selvaraj G., Zou J.
Plant Cell 15:1872-1887(2003) [PubMed: 12897259] [Abstract]
Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC008153 Genomic DNA. Translation: AAG51432.1.
CP002686 Genomic DNA. Translation: AEE75046.1.
AK117634 mRNA. Translation: BAC42290.1.
BT005134 mRNA. Translation: AAO50667.1.
IPIIPI00517423.
RefSeqNP_187750.1. NM_111976.3.
UniGeneAt.39753.

3D structure databases

ProteinModelPortalQ9CAY3.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9CAY3.

Proteomic databases

PRIDEQ9CAY3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G11430.1; AT3G11430.1; AT3G11430.
GeneID820316.
GenomeReviewsGene locus AT3G11430 in contig BA000014_GR.
KEGGath:AT3G11430.
NMPDRfig|3702.1.peg.13194.

Organism-specific databases

TAIRAt3g11430.

Phylogenomic databases

GeneTreeEPGT00050000001610.
HOGENOMHBG590278.
InParanoidQ9CAY3.
OMAMEATCSS.
PhylomeDBQ9CAY3.
ProtClustDBPLN02499.

Gene expression databases

ArrayExpressQ9CAY3.
GenevestigatorQ9CAY3.
GermOnlineAT3G11430. Arabidopsis thaliana.

Family and domain databases

InterProIPR002123. Acyltransferase.
[Graphical view]
KOK13508.
PfamPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPAT5_ARATH
AccessionPrimary (citable) accession number: Q9CAY3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2001
Last modified: December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents