Reviewed, UniProtKB/Swiss-Prot Q9CAY3 (GPAT5_ARATH)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 5
      Short name=AtGPAT5
    EC=2.3.1.15

Gene names

Name:	GPAT5
Ordered Locus Names:	At3g11430
ORF Names:	F24K9.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. Ref.4
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Weakly or not expressed in roots, leaves, seedlings, developing siliques and flower buds. Ref.4
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.
Biophysicochemical properties	Kinetic parameters: Vmax=86.85 pmol/min/mg enzyme

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW suberin biosynthetic process Inferred from mutant phenotype. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Ref.4 Inferred from genetic interaction. Source: TAIR glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glycerol-3-phosphate acyltransferase 5		PRO_0000195253
Regions
	Transmembrane	31 – 51	21	Potential
	Transmembrane	237 – 257	21	Potential
	Motif	300 – 305	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q9CAY3-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 32E612BD3A21CD6E	FASTA	502	56,081
	10 20 30 40 50 60 MVMEQAGTTS YSVVSEFEGT ILKNADSFSY FMLVAFEAAG LIRFAILLFL WPVITLLDVF 70 80 90 100 110 120 SYKNAALKLK IFVATVGLRE PEIESVARAV LPKFYMDDVS MDTWRVFSSC KKRVVVTRMP 130 140 150 160 170 180 RVMVERFAKE HLRADEVIGT ELIVNRFGFV TGLIRETDVD QSALNRVANL FVGRRPQLGL 190 200 210 220 230 240 GKPALTASTN FLSLCEEHIH APIPENYNHG DQQLQLRPLP VIFHDGRLVK RPTPATALII 250 260 270 280 290 300 LLWIPFGIIL AVIRIFLGAV LPLWATPYVS QIFGGHIIVK GKPPQPPAAG KSGVLFVCTH 310 320 330 340 350 360 RTLMDPVVLS YVLGRSIPAV TYSISRLSEI LSPIPTVRLT RIRDVDAAKI KQQLSKGDLV 370 380 390 400 410 420 VCPEGTTCRE PFLLRFSALF AELTDRIVPV AMNYRVGFFH ATTARGWKGL DPIFFFMNPR 430 440 450 460 470 480 PVYEITFLNQ LPMEATCSSG KSPHDVANYV QRILAATLGF ECTNFTRKDK YRVLAGNDGT 490 500 VSYLSLLDQL KKVVSTFEPC LH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility." Zheng Z., Xia Q., Dauk M., Shen W., Selvaraj G., Zou J. Plant Cell 15:1872-1887(2003) [PubMed: 12897259] [Abstract] Cited for: ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008153 Genomic DNA. Translation: AAG51432.1. AK117634 mRNA. Translation: BAC42290.1. BT005134 mRNA. Translation: AAO50667.1.
IPI	IPI00517423.
RefSeq	NP_187750.1.
UniGene	At.39753
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	820316.
GenomeReviews	Gene locus AT3G11430 in contig BA000014_GR.
KEGG	ath:AT3G11430.
NMPDR	fig|3702.1.peg.13194.
Organism-specific databases
TAIR	At3g11430.
Phylogenomic databases
HOGENOM	HBG590278.
InParanoid	Q9CAY3.
OMA	AFVRIAV.
Enzyme and pathway databases
BRENDA	2.3.1.15. 302.
Gene expression databases
ArrayExpress	Q9CAY3.
Genevestigator	Q9CAY3.
GermOnline	AT3G11430. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT5_ARATH

Accession

Primary (citable) accession number: Q9CAY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)