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Protein

Long chain acyl-CoA synthetase 9, chloroplastic

Gene

LACS9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 261ATPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • fatty acid metabolic process Source: TAIR

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G77590-MONOMER
MetaCyc:AT1G77590-MONOMER
ReactomeiR-ATH-75876 Synthesis of very long-chain fatty acyl-CoAs
SABIO-RKQ9CAP8
UniPathwayiUPA00199

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 9, chloroplastic (EC:6.2.1.3)
Gene namesi
Name:LACS9
Ordered Locus Names:At1g77590
ORF Names:T5M16.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G77590
TAIRilocus:2204765 AT1G77590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced amount long-chain fatty acid (LCFA).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004014181 – 691Long chain acyl-CoA synthetase 9, chloroplasticAdd BLAST691

Proteomic databases

PaxDbiQ9CAP8
PRIDEiQ9CAP8

PTM databases

iPTMnetiQ9CAP8

Expressioni

Tissue specificityi

Highly expressed in developing seeds and young rosette leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9CAP8 baseline and differential
GenevisibleiQ9CAP8 AT

Interactioni

Protein-protein interaction databases

BioGridi29313, 1 interactor
STRINGi3702.AT1G77590.1

Structurei

3D structure databases

ProteinModelPortaliQ9CAP8
SMRiQ9CAP8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni525 – 553Fatty acid-bindingSequence analysisAdd BLAST29

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1180 Eukaryota
COG1022 LUCA
HOGENOMiHOG000159459
InParanoidiQ9CAP8
KOiK01897
OMAiGPLVCCE
OrthoDBiEOG093603EC
PhylomeDBiQ9CAP8

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CAP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPYAAGVIV PLALTFLVQK SKKEKKRGVV VDVGGEPGYA IRNHRFTEPV
60 70 80 90 100
SSHWEHISTL PELFEISCNA HSDRVFLGTR KLISREIETS EDGKTFEKLH
110 120 130 140 150
LGDYEWLTFG KTLEAVCDFA SGLVQIGHKT EERVAIFADT REEWFISLQG
160 170 180 190 200
CFRRNVTVVT IYSSLGEEAL CHSLNETEVT TVICGSKELK KLMDISQQLE
210 220 230 240 250
TVKRVICMDD EFPSDVNSNW MATSFTDVQK LGRENPVDPN FPLSADVAVI
260 270 280 290 300
MYTSGSTGLP KGVMMTHGNV LATVSAVMTI VPDLGKRDIY MAYLPLAHIL
310 320 330 340 350
ELAAESVMAT IGSAIGYGSP LTLTDTSNKI KKGTKGDVTA LKPTIMTAVP
360 370 380 390 400
AILDRVRDGV RKKVDAKGGL SKKLFDFAYA RRLSAINGSW FGAWGLEKLL
410 420 430 440 450
WDVLVFRKIR AVLGGQIRYL LSGGAPLSGD TQRFINICVG APIGQGYGLT
460 470 480 490 500
ETCAGGTFSE FEDTSVGRVG APLPCSFVKL VDWAEGGYLT SDKPMPRGEI
510 520 530 540 550
VIGGSNITLG YFKNEEKTKE VYKVDEKGMR WFYTGDIGRF HPDGCLEIID
560 570 580 590 600
RKKDIVKLQH GEYVSLGKVE AALSISPYVE NIMVHADSFY SYCVALVVAS
610 620 630 640 650
QHTVEGWASK QGIDFANFEE LCTKEQAVKE VYASLVKAAK QSRLEKFEIP
660 670 680 690
AKIKLLASPW TPESGLVTAA LKLKRDVIRR EFSEDLTKLY A
Length:691
Mass (Da):76,176
Last modified:June 1, 2001 - v1
Checksum:i8B01B936CF91F564
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293Y → F in AAO22689 (PubMed:14593172).Curated1
Sequence conflicti586 – 587AD → SS in BAF01957 (Ref. 5) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503759 mRNA Translation: AAM28876.1
AC010704 Genomic DNA Translation: AAG51668.1
CP002684 Genomic DNA Translation: AEE35996.1
BT002872 mRNA Translation: AAO22689.1
AK230143 mRNA Translation: BAF01957.1
PIRiD96805
RefSeqiNP_177882.1, NM_106407.7
UniGeneiAt.28137

Genome annotation databases

EnsemblPlantsiAT1G77590.1; AT1G77590.1; AT1G77590
GeneIDi844094
GrameneiAT1G77590.1; AT1G77590.1; AT1G77590
KEGGiath:AT1G77590

Similar proteinsi

Entry informationi

Entry nameiLACS9_ARATH
AccessioniPrimary (citable) accession number: Q9CAP8
Secondary accession number(s): Q0WLQ0, Q84WQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health