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Q9CAP8

- LACS9_ARATH

UniProt

Q9CAP8 - LACS9_ARATH

Protein

Long chain acyl-CoA synthetase 9, chloroplastic

Gene

LACS9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.1 Publication

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi250 – 26112ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: TAIR

    GO - Biological processi

    1. fatty acid metabolic process Source: TAIR
    2. long-chain fatty acid metabolic process Source: GOC

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G77590-MONOMER.
    MetaCyc:AT1G77590-MONOMER.
    SABIO-RKQ9CAP8.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 9, chloroplastic (EC:6.2.1.3)
    Gene namesi
    Name:LACS9
    Ordered Locus Names:At1g77590
    ORF Names:T5M16.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G77590.

    Subcellular locationi

    Plastidchloroplast envelope 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. membrane Source: TAIR
    4. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Reduced amount long-chain fatty acid (LCFA).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691Long chain acyl-CoA synthetase 9, chloroplasticPRO_0000401418Add
    BLAST

    Proteomic databases

    PaxDbiQ9CAP8.
    PRIDEiQ9CAP8.

    Expressioni

    Tissue specificityi

    Highly expressed in developing seeds and young rosette leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ9CAP8.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G77590.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CAP8.
    SMRiQ9CAP8. Positions 105-683.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni525 – 55329Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ9CAP8.
    KOiK01897.
    OMAiASWEVDA.
    PhylomeDBiQ9CAP8.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CAP8-1 [UniParc]FASTAAdd to Basket

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    MIPYAAGVIV PLALTFLVQK SKKEKKRGVV VDVGGEPGYA IRNHRFTEPV    50
    SSHWEHISTL PELFEISCNA HSDRVFLGTR KLISREIETS EDGKTFEKLH 100
    LGDYEWLTFG KTLEAVCDFA SGLVQIGHKT EERVAIFADT REEWFISLQG 150
    CFRRNVTVVT IYSSLGEEAL CHSLNETEVT TVICGSKELK KLMDISQQLE 200
    TVKRVICMDD EFPSDVNSNW MATSFTDVQK LGRENPVDPN FPLSADVAVI 250
    MYTSGSTGLP KGVMMTHGNV LATVSAVMTI VPDLGKRDIY MAYLPLAHIL 300
    ELAAESVMAT IGSAIGYGSP LTLTDTSNKI KKGTKGDVTA LKPTIMTAVP 350
    AILDRVRDGV RKKVDAKGGL SKKLFDFAYA RRLSAINGSW FGAWGLEKLL 400
    WDVLVFRKIR AVLGGQIRYL LSGGAPLSGD TQRFINICVG APIGQGYGLT 450
    ETCAGGTFSE FEDTSVGRVG APLPCSFVKL VDWAEGGYLT SDKPMPRGEI 500
    VIGGSNITLG YFKNEEKTKE VYKVDEKGMR WFYTGDIGRF HPDGCLEIID 550
    RKKDIVKLQH GEYVSLGKVE AALSISPYVE NIMVHADSFY SYCVALVVAS 600
    QHTVEGWASK QGIDFANFEE LCTKEQAVKE VYASLVKAAK QSRLEKFEIP 650
    AKIKLLASPW TPESGLVTAA LKLKRDVIRR EFSEDLTKLY A 691
    Length:691
    Mass (Da):76,176
    Last modified:June 1, 2001 - v1
    Checksum:i8B01B936CF91F564
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti293 – 2931Y → F in AAO22689. (PubMed:14593172)Curated
    Sequence conflicti586 – 5872AD → SS in BAF01957. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503759 mRNA. Translation: AAM28876.1.
    AC010704 Genomic DNA. Translation: AAG51668.1.
    CP002684 Genomic DNA. Translation: AEE35996.1.
    BT002872 mRNA. Translation: AAO22689.1.
    AK230143 mRNA. Translation: BAF01957.1.
    PIRiD96805.
    RefSeqiNP_177882.1. NM_106407.6.
    UniGeneiAt.28137.

    Genome annotation databases

    EnsemblPlantsiAT1G77590.1; AT1G77590.1; AT1G77590.
    GeneIDi844094.
    KEGGiath:AT1G77590.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503759 mRNA. Translation: AAM28876.1 .
    AC010704 Genomic DNA. Translation: AAG51668.1 .
    CP002684 Genomic DNA. Translation: AEE35996.1 .
    BT002872 mRNA. Translation: AAO22689.1 .
    AK230143 mRNA. Translation: BAF01957.1 .
    PIRi D96805.
    RefSeqi NP_177882.1. NM_106407.6.
    UniGenei At.28137.

    3D structure databases

    ProteinModelPortali Q9CAP8.
    SMRi Q9CAP8. Positions 105-683.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G77590.1-P.

    Proteomic databases

    PaxDbi Q9CAP8.
    PRIDEi Q9CAP8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G77590.1 ; AT1G77590.1 ; AT1G77590 .
    GeneIDi 844094.
    KEGGi ath:AT1G77590.

    Organism-specific databases

    TAIRi AT1G77590.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q9CAP8.
    KOi K01897.
    OMAi ASWEVDA.
    PhylomeDBi Q9CAP8.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT1G77590-MONOMER.
    MetaCyc:AT1G77590-MONOMER.
    SABIO-RK Q9CAP8.

    Gene expression databases

    Genevestigatori Q9CAP8.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-691.
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 586-691.
      Strain: cv. Columbia.
    6. "Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis."
      Schnurr J.A., Shockey J.M., de Boer G.J., Browse J.A.
      Plant Physiol. 129:1700-1709(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, DISRUPTION PHENOTYPE.
    7. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.

    Entry informationi

    Entry nameiLACS9_ARATH
    AccessioniPrimary (citable) accession number: Q9CAP8
    Secondary accession number(s): Q0WLQ0, Q84WQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3