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Q9CAP8

- LACS9_ARATH

UniProt

Q9CAP8 - LACS9_ARATH

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Protein

Long chain acyl-CoA synthetase 9, chloroplastic

Gene

LACS9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi250 – 26112ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: TAIR

GO - Biological processi

  1. fatty acid metabolic process Source: TAIR
  2. long-chain fatty acid metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G77590-MONOMER.
MetaCyc:AT1G77590-MONOMER.
ReactomeiREACT_235205. Synthesis of very long-chain fatty acyl-CoAs.
SABIO-RKQ9CAP8.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 9, chloroplastic (EC:6.2.1.3)
Gene namesi
Name:LACS9
Ordered Locus Names:At1g77590
ORF Names:T5M16.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G77590.

Subcellular locationi

Plastidchloroplast envelope 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. membrane Source: TAIR
  4. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced amount long-chain fatty acid (LCFA).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691Long chain acyl-CoA synthetase 9, chloroplasticPRO_0000401418Add
BLAST

Proteomic databases

PaxDbiQ9CAP8.
PRIDEiQ9CAP8.

Expressioni

Tissue specificityi

Highly expressed in developing seeds and young rosette leaves.1 Publication

Gene expression databases

GenevestigatoriQ9CAP8.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G77590.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9CAP8.
SMRiQ9CAP8. Positions 105-683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni525 – 55329Fatty acid-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ9CAP8.
KOiK01897.
OMAiASWEVDA.
PhylomeDBiQ9CAP8.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CAP8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIPYAAGVIV PLALTFLVQK SKKEKKRGVV VDVGGEPGYA IRNHRFTEPV
60 70 80 90 100
SSHWEHISTL PELFEISCNA HSDRVFLGTR KLISREIETS EDGKTFEKLH
110 120 130 140 150
LGDYEWLTFG KTLEAVCDFA SGLVQIGHKT EERVAIFADT REEWFISLQG
160 170 180 190 200
CFRRNVTVVT IYSSLGEEAL CHSLNETEVT TVICGSKELK KLMDISQQLE
210 220 230 240 250
TVKRVICMDD EFPSDVNSNW MATSFTDVQK LGRENPVDPN FPLSADVAVI
260 270 280 290 300
MYTSGSTGLP KGVMMTHGNV LATVSAVMTI VPDLGKRDIY MAYLPLAHIL
310 320 330 340 350
ELAAESVMAT IGSAIGYGSP LTLTDTSNKI KKGTKGDVTA LKPTIMTAVP
360 370 380 390 400
AILDRVRDGV RKKVDAKGGL SKKLFDFAYA RRLSAINGSW FGAWGLEKLL
410 420 430 440 450
WDVLVFRKIR AVLGGQIRYL LSGGAPLSGD TQRFINICVG APIGQGYGLT
460 470 480 490 500
ETCAGGTFSE FEDTSVGRVG APLPCSFVKL VDWAEGGYLT SDKPMPRGEI
510 520 530 540 550
VIGGSNITLG YFKNEEKTKE VYKVDEKGMR WFYTGDIGRF HPDGCLEIID
560 570 580 590 600
RKKDIVKLQH GEYVSLGKVE AALSISPYVE NIMVHADSFY SYCVALVVAS
610 620 630 640 650
QHTVEGWASK QGIDFANFEE LCTKEQAVKE VYASLVKAAK QSRLEKFEIP
660 670 680 690
AKIKLLASPW TPESGLVTAA LKLKRDVIRR EFSEDLTKLY A
Length:691
Mass (Da):76,176
Last modified:June 1, 2001 - v1
Checksum:i8B01B936CF91F564
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931Y → F in AAO22689. (PubMed:14593172)Curated
Sequence conflicti586 – 5872AD → SS in BAF01957. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503759 mRNA. Translation: AAM28876.1.
AC010704 Genomic DNA. Translation: AAG51668.1.
CP002684 Genomic DNA. Translation: AEE35996.1.
BT002872 mRNA. Translation: AAO22689.1.
AK230143 mRNA. Translation: BAF01957.1.
PIRiD96805.
RefSeqiNP_177882.1. NM_106407.6.
UniGeneiAt.28137.

Genome annotation databases

EnsemblPlantsiAT1G77590.1; AT1G77590.1; AT1G77590.
GeneIDi844094.
KEGGiath:AT1G77590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503759 mRNA. Translation: AAM28876.1 .
AC010704 Genomic DNA. Translation: AAG51668.1 .
CP002684 Genomic DNA. Translation: AEE35996.1 .
BT002872 mRNA. Translation: AAO22689.1 .
AK230143 mRNA. Translation: BAF01957.1 .
PIRi D96805.
RefSeqi NP_177882.1. NM_106407.6.
UniGenei At.28137.

3D structure databases

ProteinModelPortali Q9CAP8.
SMRi Q9CAP8. Positions 105-683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G77590.1-P.

Proteomic databases

PaxDbi Q9CAP8.
PRIDEi Q9CAP8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G77590.1 ; AT1G77590.1 ; AT1G77590 .
GeneIDi 844094.
KEGGi ath:AT1G77590.

Organism-specific databases

TAIRi AT1G77590.

Phylogenomic databases

eggNOGi COG1022.
HOGENOMi HOG000159459.
InParanoidi Q9CAP8.
KOi K01897.
OMAi ASWEVDA.
PhylomeDBi Q9CAP8.

Enzyme and pathway databases

UniPathwayi UPA00199 .
BioCyci ARA:AT1G77590-MONOMER.
MetaCyc:AT1G77590-MONOMER.
Reactomei REACT_235205. Synthesis of very long-chain fatty acyl-CoAs.
SABIO-RK Q9CAP8.

Gene expression databases

Genevestigatori Q9CAP8.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-691.
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 586-691.
    Strain: cv. Columbia.
  6. "Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis."
    Schnurr J.A., Shockey J.M., de Boer G.J., Browse J.A.
    Plant Physiol. 129:1700-1709(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, DISRUPTION PHENOTYPE.
  7. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.

Entry informationi

Entry nameiLACS9_ARATH
AccessioniPrimary (citable) accession number: Q9CAP8
Secondary accession number(s): Q0WLQ0, Q84WQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3