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Q9CAP8 (LACS9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long chain acyl-CoA synthetase 9, chloroplastic

EC=6.2.1.3
Gene names
Name:LACS9
Ordered Locus Names:At1g77590
ORF Names:T5M16.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate and linoleate. Ref.6

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. Ref.1

Cofactor

Magnesium By similarity.

Pathway

Lipid metabolism; fatty acid metabolism.

Subcellular location

Plastidchloroplast envelope Ref.6.

Tissue specificity

Highly expressed in developing seeds and young rosette leaves. Ref.6

Disruption phenotype

Reduced amount long-chain fatty acid (LCFA). Ref.6

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Long chain acyl-CoA synthetase 9, chloroplastic
PRO_0000401418

Regions

Nucleotide binding250 – 26112ATP Potential
Region525 – 55329Fatty acid-binding Potential

Experimental info

Sequence conflict2931Y → F in AAO22689. Ref.4
Sequence conflict586 – 5872AD → SS in BAF01957. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9CAP8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 8B01B936CF91F564

FASTA69176,176
        10         20         30         40         50         60 
MIPYAAGVIV PLALTFLVQK SKKEKKRGVV VDVGGEPGYA IRNHRFTEPV SSHWEHISTL 

        70         80         90        100        110        120 
PELFEISCNA HSDRVFLGTR KLISREIETS EDGKTFEKLH LGDYEWLTFG KTLEAVCDFA 

       130        140        150        160        170        180 
SGLVQIGHKT EERVAIFADT REEWFISLQG CFRRNVTVVT IYSSLGEEAL CHSLNETEVT 

       190        200        210        220        230        240 
TVICGSKELK KLMDISQQLE TVKRVICMDD EFPSDVNSNW MATSFTDVQK LGRENPVDPN 

       250        260        270        280        290        300 
FPLSADVAVI MYTSGSTGLP KGVMMTHGNV LATVSAVMTI VPDLGKRDIY MAYLPLAHIL 

       310        320        330        340        350        360 
ELAAESVMAT IGSAIGYGSP LTLTDTSNKI KKGTKGDVTA LKPTIMTAVP AILDRVRDGV 

       370        380        390        400        410        420 
RKKVDAKGGL SKKLFDFAYA RRLSAINGSW FGAWGLEKLL WDVLVFRKIR AVLGGQIRYL 

       430        440        450        460        470        480 
LSGGAPLSGD TQRFINICVG APIGQGYGLT ETCAGGTFSE FEDTSVGRVG APLPCSFVKL 

       490        500        510        520        530        540 
VDWAEGGYLT SDKPMPRGEI VIGGSNITLG YFKNEEKTKE VYKVDEKGMR WFYTGDIGRF 

       550        560        570        580        590        600 
HPDGCLEIID RKKDIVKLQH GEYVSLGKVE AALSISPYVE NIMVHADSFY SYCVALVVAS 

       610        620        630        640        650        660 
QHTVEGWASK QGIDFANFEE LCTKEQAVKE VYASLVKAAK QSRLEKFEIP AKIKLLASPW 

       670        680        690 
TPESGLVTAA LKLKRDVIRR EFSEDLTKLY A 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
Shockey J.M., Fulda M.S., Browse J.A.
Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 293-691.
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 586-691.
Strain: cv. Columbia.
[6]"Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis."
Schnurr J.A., Shockey J.M., de Boer G.J., Browse J.A.
Plant Physiol. 129:1700-1709(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, DISRUPTION PHENOTYPE.
[7]"Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
Shockey J.M., Fulda M.S., Browse J.
Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF503759 mRNA. Translation: AAM28876.1.
AC010704 Genomic DNA. Translation: AAG51668.1.
CP002684 Genomic DNA. Translation: AEE35996.1.
BT002872 mRNA. Translation: AAO22689.1.
AK230143 mRNA. Translation: BAF01957.1.
PIRD96805.
RefSeqNP_177882.1. NM_106407.6.
UniGeneAt.28137.

3D structure databases

ProteinModelPortalQ9CAP8.
SMRQ9CAP8. Positions 105-683.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G77590.1-P.

Proteomic databases

PaxDbQ9CAP8.
PRIDEQ9CAP8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G77590.1; AT1G77590.1; AT1G77590.
GeneID844094.
KEGGath:AT1G77590.

Organism-specific databases

TAIRAT1G77590.

Phylogenomic databases

eggNOGCOG1022.
HOGENOMHOG000159459.
InParanoidQ9CAP8.
KOK01897.
OMAAHVLELC.
PhylomeDBQ9CAP8.
ProtClustDBPLN02387.

Enzyme and pathway databases

BioCycARA:AT1G77590-MONOMER.
MetaCyc:AT1G77590-MONOMER.
SABIO-RKQ9CAP8.
UniPathwayUPA00199.

Gene expression databases

GenevestigatorQ9CAP8.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACS9_ARATH
AccessionPrimary (citable) accession number: Q9CAP8
Secondary accession number(s): Q0WLQ0, Q84WQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names