Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 2C 16

Gene

HAB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Confers enhanced sensitivity to drought.4 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+2 Publications, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Enzyme regulationi

Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi243Manganese 1Combined sources1 Publication1
Metal bindingi243Manganese 2Combined sources1 Publication1
Metal bindingi244Manganese 1; via carbonyl oxygenCombined sources1 Publication1
Sitei385Lock1
Metal bindingi432Manganese 2Combined sources1 Publication1
Metal bindingi492Manganese 2Combined sources1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 16 (EC:3.1.3.16)
Short name:
AtPP2C16
Alternative name(s):
AtP2C-HA
Protein HYPERSENSITIVE TO ABA 1
Protein phosphatase 2C HAB1
Short name:
PP2C HAB1
Gene namesi
Name:HAB1
Synonyms:P2C-HA
Ordered Locus Names:At1g72770
ORF Names:F28P22.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G72770.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Mainly cytoplasmic.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi246G → D: Reduced phosphatase activity, impaired affinity for PYR/PYL/RCAR receptors, and insensitivity to ABA. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000034452423 – 511Protein phosphatase 2C 16Add BLAST489

Proteomic databases

PaxDbiQ9CAJ0.

Expressioni

Tissue specificityi

Expressed in seeds, roots, stems, leaves and flowers, especially in meristematic tissues, guard cells, embryo and siliques.3 Publications

Inductioni

Repressed by MYB44. Induced by ABA.4 Publications

Gene expression databases

GenevisibleiQ9CAJ0. AT.

Interactioni

Subunit structurei

Interacts with SWI3B (via N-terminus). Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL8 and PYL9, and with free PYL2, PYL3, PYL4, PYL10 and PYL13.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PYL1Q8VZS82EBI-2309302,EBI-2363104
PYL5Q9FLB16EBI-2309302,EBI-2363181
PYL6Q8S8E34EBI-2309302,EBI-2363192
PYL8Q9FGM13EBI-2309302,EBI-2429535
PYR1O496868EBI-2309302,EBI-2349590
SWI3BQ84JG24EBI-2309302,EBI-1102271

Protein-protein interaction databases

BioGridi28828. 25 interactors.
DIPiDIP-48988N.
IntActiQ9CAJ0. 16 interactors.
MINTiMINT-8299472.
STRINGi3702.AT1G72770.1.

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi181 – 183Combined sources3
Beta strandi190 – 195Combined sources6
Beta strandi199 – 201Combined sources3
Beta strandi204 – 216Combined sources13
Helixi217 – 219Combined sources3
Beta strandi233 – 248Combined sources16
Helixi249 – 268Combined sources20
Turni269 – 271Combined sources3
Helixi284 – 302Combined sources19
Beta strandi319 – 321Combined sources3
Beta strandi329 – 334Combined sources6
Beta strandi336 – 346Combined sources11
Beta strandi348 – 353Combined sources6
Beta strandi356 – 360Combined sources5
Helixi369 – 377Combined sources9
Beta strandi382 – 390Combined sources9
Turni391 – 393Combined sources3
Helixi403 – 405Combined sources3
Turni406 – 408Combined sources3
Beta strandi414 – 419Combined sources6
Beta strandi424 – 430Combined sources7
Helixi432 – 435Combined sources4
Helixi440 – 458Combined sources19
Turni463 – 468Combined sources6
Helixi472 – 487Combined sources16
Beta strandi494 – 500Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KB3X-ray1.95B186-506[»]
3NMTX-ray2.56B172-511[»]
3QN1X-ray1.80B178-511[»]
3RT0X-ray2.11A/B172-511[»]
3UJGX-ray2.60B172-511[»]
3ZVUX-ray2.10B178-511[»]
4DS8X-ray2.21B169-511[»]
4LA7X-ray1.98B178-505[»]
4LG5X-ray2.88B172-511[»]
4LGAX-ray2.70B172-511[»]
4LGBX-ray3.15B172-511[»]
4WVOX-ray2.25B178-505[»]
5JO1X-ray2.30B172-506[»]
5JO2X-ray2.42B172-506[»]
ProteinModelPortaliQ9CAJ0.
SMRiQ9CAJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CAJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini189 – 501PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST313

Domaini

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9CAJ0.
KOiK14497.
OMAiDELCKRN.
OrthoDBiEOG09360DDO.
PhylomeDBiQ9CAJ0.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CAJ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEMTPAVAM TLSLAANTMC ESSPVEITQL KNVTDAADLL SDSENQSFCN
60 70 80 90 100
GGTECTMEDV SELEEVGEQD LLKTLSDTRS GSSNVFDEDD VLSVVEDNSA
110 120 130 140 150
VISEGLLVVD AGSELSLSNT AMEIDNGRVL ATAIIVGESS IEQVPTAEVL
160 170 180 190 200
IAGVNQDTNT SEVVIRLPDE NSNHLVKGRS VYELDCIPLW GTVSIQGNRS
210 220 230 240 250
EMEDAFAVSP HFLKLPIKML MGDHEGMSPS LTHLTGHFFG VYDGHGGHKV
260 270 280 290 300
ADYCRDRLHF ALAEEIERIK DELCKRNTGE GRQVQWDKVF TSCFLTVDGE
310 320 330 340 350
IEGKIGRAVV GSSDKVLEAV ASETVGSTAV VALVCSSHIV VSNCGDSRAV
360 370 380 390 400
LFRGKEAMPL SVDHKPDRED EYARIENAGG KVIQWQGARV FGVLAMSRSI
410 420 430 440 450
GDRYLKPYVI PEPEVTFMPR SREDECLILA SDGLWDVMNN QEVCEIARRR
460 470 480 490 500
ILMWHKKNGA PPLAERGKGI DPACQAAADY LSMLALQKGS KDNISIIVID
510
LKAQRKFKTR T
Length:511
Mass (Da):55,744
Last modified:June 1, 2001 - v1
Checksum:iB001BAF0DD8333E1
GO
Isoform 2 (identifier: Q9CAJ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     402-406: DRYLK → KHCFF
     407-511: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):43,866
Checksum:i489298C9592A9F43
GO

Sequence cautioni

The sequence BAH56780 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46Q → P in CAA05875 (PubMed:9862504).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034844402 – 406DRYLK → KHCFF in isoform 2. 1 Publication5
Alternative sequenceiVSP_034845407 – 511Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003119 Genomic DNA. Translation: CAA05875.1.
AC010926 Genomic DNA. Translation: AAG51849.1.
CP002684 Genomic DNA. Translation: AEE35370.1.
CP002684 Genomic DNA. Translation: AEE35371.1.
CP002684 Genomic DNA. Translation: AEE35372.1.
BT015409 mRNA. Translation: AAU05532.1.
AK230171 mRNA. Translation: BAF01980.1.
AK318665 mRNA. Translation: BAH56780.1. Different initiation.
PIRiF96752.
RefSeqiNP_001077815.1. NM_001084346.3. [Q9CAJ0-2]
NP_001185385.1. NM_001198456.1. [Q9CAJ0-1]
NP_177421.1. NM_105936.4. [Q9CAJ0-1]
UniGeneiAt.46635.
At.67356.

Genome annotation databases

EnsemblPlantsiAT1G72770.1; AT1G72770.1; AT1G72770. [Q9CAJ0-1]
AT1G72770.3; AT1G72770.3; AT1G72770. [Q9CAJ0-1]
GeneIDi843609.
GrameneiAT1G72770.1; AT1G72770.1; AT1G72770.
AT1G72770.3; AT1G72770.3; AT1G72770.
KEGGiath:AT1G72770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003119 Genomic DNA. Translation: CAA05875.1.
AC010926 Genomic DNA. Translation: AAG51849.1.
CP002684 Genomic DNA. Translation: AEE35370.1.
CP002684 Genomic DNA. Translation: AEE35371.1.
CP002684 Genomic DNA. Translation: AEE35372.1.
BT015409 mRNA. Translation: AAU05532.1.
AK230171 mRNA. Translation: BAF01980.1.
AK318665 mRNA. Translation: BAH56780.1. Different initiation.
PIRiF96752.
RefSeqiNP_001077815.1. NM_001084346.3. [Q9CAJ0-2]
NP_001185385.1. NM_001198456.1. [Q9CAJ0-1]
NP_177421.1. NM_105936.4. [Q9CAJ0-1]
UniGeneiAt.46635.
At.67356.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KB3X-ray1.95B186-506[»]
3NMTX-ray2.56B172-511[»]
3QN1X-ray1.80B178-511[»]
3RT0X-ray2.11A/B172-511[»]
3UJGX-ray2.60B172-511[»]
3ZVUX-ray2.10B178-511[»]
4DS8X-ray2.21B169-511[»]
4LA7X-ray1.98B178-505[»]
4LG5X-ray2.88B172-511[»]
4LGAX-ray2.70B172-511[»]
4LGBX-ray3.15B172-511[»]
4WVOX-ray2.25B178-505[»]
5JO1X-ray2.30B172-506[»]
5JO2X-ray2.42B172-506[»]
ProteinModelPortaliQ9CAJ0.
SMRiQ9CAJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28828. 25 interactors.
DIPiDIP-48988N.
IntActiQ9CAJ0. 16 interactors.
MINTiMINT-8299472.
STRINGi3702.AT1G72770.1.

Proteomic databases

PaxDbiQ9CAJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G72770.1; AT1G72770.1; AT1G72770. [Q9CAJ0-1]
AT1G72770.3; AT1G72770.3; AT1G72770. [Q9CAJ0-1]
GeneIDi843609.
GrameneiAT1G72770.1; AT1G72770.1; AT1G72770.
AT1G72770.3; AT1G72770.3; AT1G72770.
KEGGiath:AT1G72770.

Organism-specific databases

TAIRiAT1G72770.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000233896.
InParanoidiQ9CAJ0.
KOiK14497.
OMAiDELCKRN.
OrthoDBiEOG09360DDO.
PhylomeDBiQ9CAJ0.

Miscellaneous databases

EvolutionaryTraceiQ9CAJ0.
PROiQ9CAJ0.

Gene expression databases

GenevisibleiQ9CAJ0. AT.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 3 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C16_ARATH
AccessioniPrimary (citable) accession number: Q9CAJ0
Secondary accession number(s): C0Z251, O81709, Q0WLM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.