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Protein

Polyprenol reductase 1

Gene

At1g72590

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_286792. Androgen biosynthesis.
REACT_324987. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase 1 (EC:1.3.1.94)
Gene namesi
Ordered Locus Names:At1g72590
ORF Names:F28P22.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G72590.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521HelicalSequence AnalysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence AnalysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Polyprenol reductase 1PRO_0000398655Add
BLAST

Proteomic databases

PRIDEiQ9CAH5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G72590.1.

Structurei

3D structure databases

ProteinModelPortaliQ9CAH5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000240963.
InParanoidiQ9CAH5.
OMAiFFEVVMY.
PhylomeDBiQ9CAH5.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CAH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVEIVWLVK AAWITVWIVS ILPLVIASIP SSKLNSFREL VLSFAGRGKI
60 70 80 90 100
LHPSSQKFTV PQKFFGHFYV VGVVWTTLLL AATWMYACKM AGGSHVFSFH
110 120 130 140 150
MTHVEHRFKV GRAVFLLLLM EIHVLRRVIE SFYVFKYSTS ARMHILAYVG
160 170 180 190 200
ALFYYVAAPL SLCSNIAPEV ARFVGSQVAE FIASGKSHSH DFNLLLSISP
210 220 230 240 250
LMKLGSLQWI GGAIFLWGWI HQRRCHAILG SLREYPSQAK EYIIPYGDWF
260 270 280 290 300
EMVSCPHFLA EIVLYLGLLI SSGGTDISIW LLFGFVAANL TYAAGETHRW
310 320
YLQKFENYPA SRHAIFPHVY
Length:320
Mass (Da):36,313
Last modified:June 1, 2001 - v1
Checksum:i326E51CF4A5719E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010926 Genomic DNA. Translation: AAG51854.1.
CP002684 Genomic DNA. Translation: AEE35345.1.
PIRiD96750.
RefSeqiNP_177403.1. NM_105918.2.
UniGeneiAt.35081.

Genome annotation databases

EnsemblPlantsiAT1G72590.1; AT1G72590.1; AT1G72590.
GeneIDi843591.
KEGGiath:AT1G72590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010926 Genomic DNA. Translation: AAG51854.1.
CP002684 Genomic DNA. Translation: AEE35345.1.
PIRiD96750.
RefSeqiNP_177403.1. NM_105918.2.
UniGeneiAt.35081.

3D structure databases

ProteinModelPortaliQ9CAH5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G72590.1.

Proteomic databases

PRIDEiQ9CAH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G72590.1; AT1G72590.1; AT1G72590.
GeneIDi843591.
KEGGiath:AT1G72590.

Organism-specific databases

TAIRiAT1G72590.

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000240963.
InParanoidiQ9CAH5.
OMAiFFEVVMY.
PhylomeDBiQ9CAH5.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_286792. Androgen biosynthesis.
REACT_324987. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

PROiQ9CAH5.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPOED1_ARATH
AccessioniPrimary (citable) accession number: Q9CAH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.