Q9CAG3 (LOX6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoxygenase 6, chloroplastic
Short name=AtLOX6
EC=1.13.11.12

Gene names

Name:	LOX6
Ordered Locus Names:	At1g67560
ORF Names:	F12B7.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure By similarity. 13S-lipoxygenase that can use linolenic acid as substrates. Ref.7
Catalytic activity	Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate. Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subcellular location	Plastid › chloroplast Potential Ref.6.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Sequence caution	The sequence BAD95111.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Oxylipin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	jasmonic acid biosynthetic process Inferred from mutant phenotype PubMed 23444343. Source: TAIR lipid oxidation Inferred from direct assay PubMed 21372125. Source: TAIR oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from direct assay Ref.6. Source: TAIR plasma membrane Inferred from direct assay PubMed 22923678. Source: TAIR
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Potential
	Chain	41 – 917	877	Lipoxygenase 6, chloroplastic		PRO_0000380595
Regions
	Domain	98 – 216	119	PLAT
	Domain	219 – 917	699	Lipoxygenase
Sites
	Metal binding	575	1	Iron; catalytic By similarity
	Metal binding	580	1	Iron; catalytic By similarity
	Metal binding	767	1	Iron; catalytic By similarity
	Metal binding	771	1	Iron; catalytic By similarity
	Metal binding	917	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CAG3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 14405AD036EC3F64	FASTA	917	104,516
	10 20 30 40 50 60 MFVASPVKTN FNGVSLVKSP AFSALSCRKQ HRVPISRQVR AVISREEKAV DQEDGKKSTN 70 80 90 100 110 120 KPLINSSQFP WQRSKYTGSK TVTAVVKIRK KIKEKLTERF EHQLELFMKA IGQGMLIQLV 130 140 150 160 170 180 SEEIDPETGK GRKSLESPVM GLPKAVKDPR YLVFTADFTV PINFGKPGAI LVTNLLSTEI 190 200 210 220 230 240 CLSEIIIEDS TDTILFPANT WIHSKNDNPQ ARIIFRSQPC LPSETPDGIK ELREKDLVSV 250 260 270 280 290 300 RGDGKGERKP HERIYDYDVY NDLGDPRKTE RVRPVLGVPE TPYPRRCRTG RPLVSKDPPC 310 320 330 340 350 360 ESRGKEKEEF YVPRDEVFEE IKRDTFRAGR FKALFHNLVP SIAAALSNLD IPFTCFSDID 370 380 390 400 410 420 NLYKSNIVLG HTEPKDTGLG GFIGGFMNGI LNVTETLLKY DTPAVIKWDR FAWLRDNEFG 430 440 450 460 470 480 RQALAGVNPV NIELLKELPI RSNLDPALYG PQESVLTEEI IAREVEHYGT TIEKALEEKR 490 500 510 520 530 540 LFLVDYHDIL LPFVEKINSI KEDPRKTYAS RTIFFYSKNG ALRPLAIELS LPPTAESENK 550 560 570 580 590 600 FVYTHGHDAT THWIWKLAKA HVCSNDAGVH QLVNHWLRTH ASMEPYIIAT NRQLSTMHPV 610 620 630 640 650 660 YKLLHPHMRY TLEINARARK SLINGGGIIE SCFTPGKYAM ELSSAAYKSM WRFDMEGLPA 670 680 690 700 710 720 DLVRRGMAEE DSSAECGVRL VIDDYPYAAD GLLIWKAIKD LVESYVKHFY SDSKSITSDL 730 740 750 760 770 780 ELQAWWDEIK NKGHYDKKDE PWWPKLNTTQ DLSQILTNMI WIASGQHAAI NFGQYPFGGY 790 800 810 820 830 840 VPNRPTLLRK LIPQETDPDY EMFMRNPQYS FLGSLPTQLQ ATKVMAVQET LSTHSPDEEY 850 860 870 880 890 900 LIELREVQRH WFQDEQVVKY FNKFSEELVK IEKTINERNK DKKLKNRTGA GMPPYELLLP 910 TSPHGVTGRG IPNSISI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AtLOX6, the fourth chloroplastic 13-LOX from Arabidopsis thaliana." Behrends V., Kunze S., Feussner I. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 564-917. Strain: cv. Columbia.
[6]	"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions." Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S. Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Diversity of the enzymatic activity in the lipoxygenase gene family of Arabidopsis thaliana." Bannenberg G., Martinez M., Hamberg M., Castresana C. Lipids 44:85-95(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ748537 mRNA. Translation: CAG38328.1. AC011020 Genomic DNA. Translation: AAG52309.1. CP002684 Genomic DNA. Translation: AEE34664.1. AY081253 mRNA. Translation: AAL91142.1. BT010546 mRNA. Translation: AAQ65169.1. AK222124 mRNA. Translation: BAD95111.1. Different initiation. AK230188 mRNA. Translation: BAF01997.1.
IPI	IPI00532662.
PIR	B96699.
RefSeq	NP_176923.1. NM_105423.2.
UniGene	At.27885.
3D structure databases
ProteinModelPortal	Q9CAG3.
SMR	Q9CAG3. Positions 105-917.
ModBase	Search...
Proteomic databases
PaxDb	Q9CAG3.
PRIDE	Q9CAG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G67560.1; AT1G67560.1; AT1G67560.
GeneID	843077.
KEGG	ath:AT1G67560.
Organism-specific databases
TAIR	At1g67560.
Phylogenomic databases
eggNOG	NOG69653.
HOGENOM	HOG000230469.
InParanoid	Q9CAG3.
KO	K00454.
OMA	TKVMAVQ.
PhylomeDB	Q9CAG3.
ProtClustDB	PLN02305.
Enzyme and pathway databases
UniPathway	UPA00382.
Gene expression databases
Genevestigator	Q9CAG3.
Family and domain databases
Gene3D	2.60.60.20. 1 hit. 4.10.372.10. 1 hit.
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. IPR027433. Lipoxygenase_domain_3. [Graphical view]
PANTHER	PTHR11771. PTHR11771. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
SUPFAM	SSF49723. Lipase_LipOase. 1 hit. SSF48484. Lipoxygenase. 1 hit.
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX6_ARATH

Accession

Primary (citable) accession number: Q9CAG3
Secondary accession number(s): Q0WLL0, Q56WC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program