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Protein

Lipoxygenase 6, chloroplastic

Gene

LOX6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity). 13S-lipoxygenase that can use linolenic acid as substrates.PROSITE-ProRule annotation1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi575Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi580Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi767Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi771Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi917Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • jasmonic acid biosynthetic process Source: CACAO
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to wounding Source: CACAO

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.12. 399.
ReactomeiR-ATH-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-ATH-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-ATH-2142700. Synthesis of Lipoxins (LX).
R-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-ATH-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-ATH-6798695. Neutrophil degranulation.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 6, chloroplastic (EC:1.13.11.12)
Short name:
AtLOX6
Gene namesi
Name:LOX6
Ordered Locus Names:At1g67560
ORF Names:F12B7.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G67560.
TAIRilocus:2008808. AT1G67560.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000038059541 – 917Lipoxygenase 6, chloroplasticAdd BLAST877

Proteomic databases

PaxDbiQ9CAG3.

PTM databases

iPTMnetiQ9CAG3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9CAG3. baseline and differential.
GenevisibleiQ9CAG3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G67560.1.

Structurei

3D structure databases

ProteinModelPortaliQ9CAG3.
SMRiQ9CAG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 216PLATPROSITE-ProRule annotationAdd BLAST119
Domaini219 – 917LipoxygenasePROSITE-ProRule annotationAdd BLAST699

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IEKP. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiQ9CAG3.
KOiK00454.
OMAiTMIWIAS.
OrthoDBiEOG0936038B.
PhylomeDBiQ9CAG3.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiView protein in InterPro
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiView protein in Pfam
PF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiView protein in SMART
SM00308. LH2. 1 hit.
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiView protein in PROSITE
PS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CAG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVASPVKTN FNGVSLVKSP AFSALSCRKQ HRVPISRQVR AVISREEKAV
60 70 80 90 100
DQEDGKKSTN KPLINSSQFP WQRSKYTGSK TVTAVVKIRK KIKEKLTERF
110 120 130 140 150
EHQLELFMKA IGQGMLIQLV SEEIDPETGK GRKSLESPVM GLPKAVKDPR
160 170 180 190 200
YLVFTADFTV PINFGKPGAI LVTNLLSTEI CLSEIIIEDS TDTILFPANT
210 220 230 240 250
WIHSKNDNPQ ARIIFRSQPC LPSETPDGIK ELREKDLVSV RGDGKGERKP
260 270 280 290 300
HERIYDYDVY NDLGDPRKTE RVRPVLGVPE TPYPRRCRTG RPLVSKDPPC
310 320 330 340 350
ESRGKEKEEF YVPRDEVFEE IKRDTFRAGR FKALFHNLVP SIAAALSNLD
360 370 380 390 400
IPFTCFSDID NLYKSNIVLG HTEPKDTGLG GFIGGFMNGI LNVTETLLKY
410 420 430 440 450
DTPAVIKWDR FAWLRDNEFG RQALAGVNPV NIELLKELPI RSNLDPALYG
460 470 480 490 500
PQESVLTEEI IAREVEHYGT TIEKALEEKR LFLVDYHDIL LPFVEKINSI
510 520 530 540 550
KEDPRKTYAS RTIFFYSKNG ALRPLAIELS LPPTAESENK FVYTHGHDAT
560 570 580 590 600
THWIWKLAKA HVCSNDAGVH QLVNHWLRTH ASMEPYIIAT NRQLSTMHPV
610 620 630 640 650
YKLLHPHMRY TLEINARARK SLINGGGIIE SCFTPGKYAM ELSSAAYKSM
660 670 680 690 700
WRFDMEGLPA DLVRRGMAEE DSSAECGVRL VIDDYPYAAD GLLIWKAIKD
710 720 730 740 750
LVESYVKHFY SDSKSITSDL ELQAWWDEIK NKGHYDKKDE PWWPKLNTTQ
760 770 780 790 800
DLSQILTNMI WIASGQHAAI NFGQYPFGGY VPNRPTLLRK LIPQETDPDY
810 820 830 840 850
EMFMRNPQYS FLGSLPTQLQ ATKVMAVQET LSTHSPDEEY LIELREVQRH
860 870 880 890 900
WFQDEQVVKY FNKFSEELVK IEKTINERNK DKKLKNRTGA GMPPYELLLP
910
TSPHGVTGRG IPNSISI
Length:917
Mass (Da):104,516
Last modified:June 1, 2001 - v1
Checksum:i14405AD036EC3F64
GO

Sequence cautioni

The sequence BAD95111 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ748537 mRNA. Translation: CAG38328.1.
AC011020 Genomic DNA. Translation: AAG52309.1.
CP002684 Genomic DNA. Translation: AEE34664.1.
AY081253 mRNA. Translation: AAL91142.1.
BT010546 mRNA. Translation: AAQ65169.1.
AK222124 mRNA. Translation: BAD95111.1. Different initiation.
AK230188 mRNA. Translation: BAF01997.1.
PIRiB96699.
RefSeqiNP_176923.1. NM_105423.3.
UniGeneiAt.27885.

Genome annotation databases

EnsemblPlantsiAT1G67560.1; AT1G67560.1; AT1G67560.
GeneIDi843077.
GrameneiAT1G67560.1; AT1G67560.1; AT1G67560.
KEGGiath:AT1G67560.

Similar proteinsi

Entry informationi

Entry nameiLOX6_ARATH
AccessioniPrimary (citable) accession number: Q9CAG3
Secondary accession number(s): Q0WLL0, Q56WC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families