Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase kinase kinase YODA

Gene

YDA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in a MAP kinase cascade that acts as a molecular switch to regulate the first cell fate decisions in the zygote and the early embryo. Promotes elongation of the zygote and development of its basal daughter cell into the extra-embryonic suspensor. In stomatal development, acts downstream of the LRR receptor TMM, but upstream of the MKK4/MKK5-MPK3/MPK6 module to regulate stomatal cell fate before the guard mother cell (GMC) is specified. Plays a central role in both guard cell identity and pattern formation. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. Upon brassinosteroid signaling, is inhibited by phosphorylation of its auto-inhibitory N-terminal domain by the GSK3-like kinase ASK7.6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Contains an N-terminal autoinhibitory domain.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei429 – 4291ATPPROSITE-ProRule annotation
Active sitei525 – 5251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi406 – 4149ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • inflorescence development Source: TAIR
  • regulation of growth Source: UniProtKB-KW
  • stomatal complex morphogenesis Source: TAIR
  • suspensor development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase

Keywords - Biological processi

Growth regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G63700-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase YODA (EC:2.7.11.25)
Alternative name(s):
YODA MAPKK kinase
Gene namesi
Name:YDA
Ordered Locus Names:At1g63700
ORF Names:F24D7.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G63700.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Severely dwarf plants that rarely survive on soil, small rosettes, compact leaves, extremely compressed shoots, and short, sterile flowers. No proper elongation of the zygote and differentiation of the extra-embryonic suspensor. Excess of stomata in leaves.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 883883Mitogen-activated protein kinase kinase kinase YODAPRO_0000422174Add
BLAST

Proteomic databases

PaxDbiQ9CAD5.
PRIDEiQ9CAD5.

PTM databases

iPTMnetiQ9CAD5.

Expressioni

Tissue specificityi

Expressed in roots, leaves, guard cells, stems, flowers and siliques.2 Publications

Gene expression databases

GenevisibleiQ9CAD5. AT.

Interactioni

Subunit structurei

Interacts with ASK7.1 Publication

Protein-protein interaction databases

BioGridi27895. 2 interactions.
DIPiDIP-59652N.
STRINGi3702.AT1G63700.1.

Structurei

3D structure databases

ProteinModelPortaliQ9CAD5.
SMRiQ9CAD5. Positions 365-675.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini400 – 656257Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 464Poly-Arg

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000083183.
InParanoidiQ9CAD5.
OMAiFGENAIR.
OrthoDBiEOG093602O9.
PhylomeDBiQ9CAD5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CAD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPWWSKSKDE KKKTNKESII DAFNRKLGFA SEDRSSGRSR KSRRRRDEIV
60 70 80 90 100
SERGAISRLP SRSPSPSTRV SRCQSFAERS PAVPLPRPIV RPHVTSTDSG
110 120 130 140 150
MNGSQRPGLD ANLKPSWLPL PKPHGATSIP DNTGAEPDFA TASVSSGSSV
160 170 180 190 200
GDIPSDSLLS PLASDCENGN RTPVNISSRD QSMHSNKNSA EMFKPVPNKN
210 220 230 240 250
RILSASPRRR PLGTHVKNLQ IPQRDLVLCS APDSLLSSPS RSPMRSFIPD
260 270 280 290 300
QVSNHGLLIS KPYSDVSLLG SGQCSSPGSG YNSGNNSIGG DMATQLFWPQ
310 320 330 340 350
SRCSPECSPV PSPRMTSPGP SSRIQSGAVT PLHPRAGGST TGSPTRRLDD
360 370 380 390 400
NRQQSHRLPL PPLLISNTCP FSPTYSAATS PSVPRSPARA EATVSPGSRW
410 420 430 440 450
KKGRLLGMGS FGHVYLGFNS ESGEMCAMKE VTLCSDDPKS RESAQQLGQE
460 470 480 490 500
ISVLSRLRHQ NIVQYYGSET VDDKLYIYLE YVSGGSIYKL LQEYGQFGEN
510 520 530 540 550
AIRNYTQQIL SGLAYLHAKN TVHRDIKGAN ILVDPHGRVK VADFGMAKHI
560 570 580 590 600
TAQSGPLSFK GSPYWMAPEV IKNSNGSNLA VDIWSLGCTV LEMATTKPPW
610 620 630 640 650
SQYEGVPAMF KIGNSKELPD IPDHLSEEGK DFVRKCLQRN PANRPTAAQL
660 670 680 690 700
LDHAFVRNVM PMERPIVSGE PAEAMNVASS TMRSLDIGHA RSLPCLDSED
710 720 730 740 750
ATNYQQKGLK HGSGFSISQS PRNMSCPISP VGSPIFHSHS PHISGRRSPS
760 770 780 790 800
PISSPHALSG SSTPLTGCGG AIPFHHQRQT TVNFLHEGIG SSRSPGSGGN
810 820 830 840 850
FYTNSFFQEP SRQQDRSRSS PRTPPHVFWD NNGSIQPGYN WNKDNQPVLS
860 870 880
DHVSQQLLSE HLKLKSLDLR PGFSTPGSTN RGP
Length:883
Mass (Da):95,935
Last modified:June 1, 2001 - v1
Checksum:i6A3FB18068F4318E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971P → A in AAR10436 (PubMed:14718171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357947 mRNA. Translation: AAR10434.1.
AY357948 Genomic DNA. Translation: AAR10435.1.
AY357949 Genomic DNA. Translation: AAR10436.1.
AC011622 Genomic DNA. Translation: AAG52426.1.
CP002684 Genomic DNA. Translation: AEE34135.1.
PIRiA96662.
RefSeqiNP_176557.1. NM_105047.2.
UniGeneiAt.21875.

Genome annotation databases

EnsemblPlantsiAT1G63700.1; AT1G63700.1; AT1G63700.
GeneIDi842674.
GrameneiAT1G63700.1; AT1G63700.1; AT1G63700.
KEGGiath:AT1G63700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357947 mRNA. Translation: AAR10434.1.
AY357948 Genomic DNA. Translation: AAR10435.1.
AY357949 Genomic DNA. Translation: AAR10436.1.
AC011622 Genomic DNA. Translation: AAG52426.1.
CP002684 Genomic DNA. Translation: AEE34135.1.
PIRiA96662.
RefSeqiNP_176557.1. NM_105047.2.
UniGeneiAt.21875.

3D structure databases

ProteinModelPortaliQ9CAD5.
SMRiQ9CAD5. Positions 365-675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27895. 2 interactions.
DIPiDIP-59652N.
STRINGi3702.AT1G63700.1.

PTM databases

iPTMnetiQ9CAD5.

Proteomic databases

PaxDbiQ9CAD5.
PRIDEiQ9CAD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G63700.1; AT1G63700.1; AT1G63700.
GeneIDi842674.
GrameneiAT1G63700.1; AT1G63700.1; AT1G63700.
KEGGiath:AT1G63700.

Organism-specific databases

TAIRiAT1G63700.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000083183.
InParanoidiQ9CAD5.
OMAiFGENAIR.
OrthoDBiEOG093602O9.
PhylomeDBiQ9CAD5.

Enzyme and pathway databases

BioCyciARA:AT1G63700-MONOMER.

Miscellaneous databases

PROiQ9CAD5.

Gene expression databases

GenevisibleiQ9CAD5. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYODA_ARATH
AccessioniPrimary (citable) accession number: Q9CAD5
Secondary accession number(s): Q6UY78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

N-terminal deletions of YDA results in gain-of-function alleles with phenotypes (no stomata and exaggerated suspensor growth) opposite to loss-of-function phenotypes.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.