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Protein

Mitogen-activated protein kinase kinase kinase YODA

Gene

YDA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in a MAP kinase cascade that acts as a molecular switch to regulate the first cell fate decisions in the zygote and the early embryo. Promotes elongation of the zygote and development of its basal daughter cell into the extra-embryonic suspensor. In stomatal development, acts downstream of the LRR receptor TMM, but upstream of the MKK4/MKK5-MPK3/MPK6 module to regulate stomatal cell fate before the guard mother cell (GMC) is specified. Plays a central role in both guard cell identity and pattern formation. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. Upon brassinosteroid signaling, is inhibited by phosphorylation of its auto-inhibitory N-terminal domain by the GSK3-like kinase ASK7.6 Publications

Miscellaneous

N-terminal deletions of YDA results in gain-of-function alleles with phenotypes (no stomata and exaggerated suspensor growth) opposite to loss-of-function phenotypes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Contains an N-terminal autoinhibitory domain.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei429ATPPROSITE-ProRule annotation1
Active sitei525Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi406 – 414ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase activity Source: GO_Central
  • inflorescence development Source: TAIR
  • regulation of growth Source: UniProtKB-KW
  • regulation of mitotic cell cycle Source: GO_Central
  • signal transduction by protein phosphorylation Source: GO_Central
  • stomatal complex morphogenesis Source: TAIR
  • stress-activated protein kinase signaling cascade Source: GO_Central
  • suspensor development Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase
Biological processGrowth regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase YODA (EC:2.7.11.25)
Alternative name(s):
YODA MAPKK kinase
Gene namesi
Name:YDA
Ordered Locus Names:At1g63700
ORF Names:F24D7.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G63700
TAIRilocus:2026674 AT1G63700

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Severely dwarf plants that rarely survive on soil, small rosettes, compact leaves, extremely compressed shoots, and short, sterile flowers. No proper elongation of the zygote and differentiation of the extra-embryonic suspensor. Excess of stomata in leaves.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221741 – 883Mitogen-activated protein kinase kinase kinase YODAAdd BLAST883

Proteomic databases

PaxDbiQ9CAD5
PRIDEiQ9CAD5

PTM databases

iPTMnetiQ9CAD5

Expressioni

Tissue specificityi

Expressed in roots, leaves, guard cells, stems, flowers and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ9CAD5 baseline and differential
GenevisibleiQ9CAD5 AT

Interactioni

Subunit structurei

Interacts with ASK7 (PubMed:22307275). Interacts with BSK12/SSP (PubMed:28821747).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK7Q390114EBI-15967064,EBI-1798250

Protein-protein interaction databases

BioGridi27895, 2 interactors
DIPiDIP-59652N
IntActiQ9CAD5, 1 interactor
STRINGi3702.AT1G63700.1

Structurei

3D structure databases

ProteinModelPortaliQ9CAD5
SMRiQ9CAD5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini400 – 656Protein kinasePROSITE-ProRule annotationAdd BLAST257

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi43 – 46Poly-Arg4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
ENOG410XQGS LUCA
HOGENOMiHOG000083183
InParanoidiQ9CAD5
KOiK20717
OMAiMHSNKNS
OrthoDBiEOG093602O9
PhylomeDBiQ9CAD5

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CAD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPWWSKSKDE KKKTNKESII DAFNRKLGFA SEDRSSGRSR KSRRRRDEIV
60 70 80 90 100
SERGAISRLP SRSPSPSTRV SRCQSFAERS PAVPLPRPIV RPHVTSTDSG
110 120 130 140 150
MNGSQRPGLD ANLKPSWLPL PKPHGATSIP DNTGAEPDFA TASVSSGSSV
160 170 180 190 200
GDIPSDSLLS PLASDCENGN RTPVNISSRD QSMHSNKNSA EMFKPVPNKN
210 220 230 240 250
RILSASPRRR PLGTHVKNLQ IPQRDLVLCS APDSLLSSPS RSPMRSFIPD
260 270 280 290 300
QVSNHGLLIS KPYSDVSLLG SGQCSSPGSG YNSGNNSIGG DMATQLFWPQ
310 320 330 340 350
SRCSPECSPV PSPRMTSPGP SSRIQSGAVT PLHPRAGGST TGSPTRRLDD
360 370 380 390 400
NRQQSHRLPL PPLLISNTCP FSPTYSAATS PSVPRSPARA EATVSPGSRW
410 420 430 440 450
KKGRLLGMGS FGHVYLGFNS ESGEMCAMKE VTLCSDDPKS RESAQQLGQE
460 470 480 490 500
ISVLSRLRHQ NIVQYYGSET VDDKLYIYLE YVSGGSIYKL LQEYGQFGEN
510 520 530 540 550
AIRNYTQQIL SGLAYLHAKN TVHRDIKGAN ILVDPHGRVK VADFGMAKHI
560 570 580 590 600
TAQSGPLSFK GSPYWMAPEV IKNSNGSNLA VDIWSLGCTV LEMATTKPPW
610 620 630 640 650
SQYEGVPAMF KIGNSKELPD IPDHLSEEGK DFVRKCLQRN PANRPTAAQL
660 670 680 690 700
LDHAFVRNVM PMERPIVSGE PAEAMNVASS TMRSLDIGHA RSLPCLDSED
710 720 730 740 750
ATNYQQKGLK HGSGFSISQS PRNMSCPISP VGSPIFHSHS PHISGRRSPS
760 770 780 790 800
PISSPHALSG SSTPLTGCGG AIPFHHQRQT TVNFLHEGIG SSRSPGSGGN
810 820 830 840 850
FYTNSFFQEP SRQQDRSRSS PRTPPHVFWD NNGSIQPGYN WNKDNQPVLS
860 870 880
DHVSQQLLSE HLKLKSLDLR PGFSTPGSTN RGP
Length:883
Mass (Da):95,935
Last modified:June 1, 2001 - v1
Checksum:i6A3FB18068F4318E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197P → A in AAR10436 (PubMed:14718171).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357947 mRNA Translation: AAR10434.1
AY357948 Genomic DNA Translation: AAR10435.1
AY357949 Genomic DNA Translation: AAR10436.1
AC011622 Genomic DNA Translation: AAG52426.1
CP002684 Genomic DNA Translation: AEE34135.1
CP002684 Genomic DNA Translation: ANM60901.1
PIRiA96662
RefSeqiNP_001319310.1, NM_001334122.1
NP_176557.1, NM_105047.2
UniGeneiAt.21875

Genome annotation databases

EnsemblPlantsiAT1G63700.1; AT1G63700.1; AT1G63700
AT1G63700.2; AT1G63700.2; AT1G63700
GeneIDi842674
GrameneiAT1G63700.1; AT1G63700.1; AT1G63700
AT1G63700.2; AT1G63700.2; AT1G63700
KEGGiath:AT1G63700

Similar proteinsi

Entry informationi

Entry nameiYODA_ARATH
AccessioniPrimary (citable) accession number: Q9CAD5
Secondary accession number(s): Q6UY78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health