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Protein

Endoglucanase 8

Gene

CEL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for cellulose formation of the cell wall.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei409By similarity1
Active sitei460By similarity1
Active sitei469By similarity1

GO - Molecular functioni

  • cellulase activity Source: TAIR

GO - Biological processi

  • cellulose catabolic process Source: UniProtKB-KW
  • cell wall modification involved in multidimensional cell growth Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G70710-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 8 (EC:3.2.1.4)
Alternative name(s):
Cellulase 1
Short name:
AtCEL1
Endo-1,4-beta glucanase 8
Gene namesi
Name:CEL1
Ordered Locus Names:At1g70710
ORF Names:F5A18.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G70710.
TAIRilocus:2033600. AT1G70710.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • extracellular region Source: UniProtKB-SubCell

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000024926125 – 492Endoglucanase 8Add BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi453N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9CAC1.

Expressioni

Tissue specificityi

Expressed in young expanding tissues. Expressed in xylem cells, young epidermal cells and newly formed cell walls.2 Publications

Gene expression databases

ExpressionAtlasiQ9CAC1. baseline and differential.
GenevisibleiQ9CAC1. AT.

Interactioni

Protein-protein interaction databases

BioGridi28628. 2 interactors.
MINTiMINT-8068205.
STRINGi3702.AT1G70710.1.

Structurei

3D structure databases

ProteinModelPortaliQ9CAC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IISA. Eukaryota.
ENOG410YBFA. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ9CAC1.
OMAiAFRDHAC.
OrthoDBiEOG093608GU.
PhylomeDBiQ9CAC1.

Family and domain databases

InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
PfamiView protein in Pfam
PF00759. Glyco_hydro_9. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CAC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKSLIFPV ILLAVLLFSP PIYSAGHDYR DALRKSILFF EGQRSGKLPP
60 70 80 90 100
DQRLKWRRDS ALRDGSSAGV DLSGGYYDAG DNIKFGFPMA FTTTMLSWSI
110 120 130 140 150
IDFGKTMGPE LRNAVKAVKW GTDYLLKATA IPGVVFVQVG DAYSDHNCWE
160 170 180 190 200
RPEDMDTLRT VYKIDRAHPG SDVAGETAAA LAAASIVFRK RDPAYSRLLL
210 220 230 240 250
DRATRVFAFA NRYRGAYSNS LYHAVCPFYC DFNGYQDELL WGAAWLHKAS
260 270 280 290 300
RKRAYREFIV KNEVILKAGD TINEFGWDNK HAGINVLISK EVLMGKAEYF
310 320 330 340 350
ESFKQNADGF ICSILPGISH PQVQYSRGGL LVKTGGSNMQ HVTSLSFLLL
360 370 380 390 400
AYSNYLSHAK KVVPCGELTA SPSLLRQIAK RQVDYILGDN PMGLSYMVGY
410 420 430 440 450
GQKFPRRIHH RGSSVPSVSA HPSHIGCKEG SRYFLSPNPN PNLLVGAVVG
460 470 480 490
GPNVTDAFPD SRPYFQQSEP TTYINAPLVG LLGYFSAHST WR
Length:492
Mass (Da):54,610
Last modified:June 1, 2001 - v1
Checksum:i7FDA753A52B97E88
GO

Sequence cautioni

The sequence CAA67156 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13L → H in CAA67157 (PubMed:9290636).Curated1
Sequence conflicti71D → N in CAA67156 (PubMed:9290636).Curated1
Sequence conflicti225V → D in CAA67157 (PubMed:9290636).Curated1
Sequence conflicti444L → W in CAA67156 (PubMed:9290636).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98543 Genomic DNA. Translation: CAA67156.1. Sequence problems.
X98544 mRNA. Translation: CAA67157.1.
AC011663 Genomic DNA. Translation: AAG52329.1.
CP002684 Genomic DNA. Translation: AEE35103.1.
AY048283 mRNA. Translation: AAK82545.1.
AY074552 mRNA. Translation: AAL67092.1.
PIRiE96731.
RefSeqiNP_177228.1. NM_105739.4.
UniGeneiAt.21900.
At.72497.

Genome annotation databases

EnsemblPlantsiAT1G70710.1; AT1G70710.1; AT1G70710.
GeneIDi843408.
GrameneiAT1G70710.1; AT1G70710.1; AT1G70710.
KEGGiath:AT1G70710.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGUN8_ARATH
AccessioniPrimary (citable) accession number: Q9CAC1
Secondary accession number(s): O23696, O23697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: June 7, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families