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Q9CAC1

- GUN8_ARATH

UniProt

Q9CAC1 - GUN8_ARATH

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Protein

Endoglucanase 8

Gene

CEL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Required for cellulose formation of the cell wall.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091By similarity
Active sitei460 – 4601By similarity
Active sitei469 – 4691By similarity

GO - Molecular functioni

  1. cellulase activity Source: TAIR

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall modification involved in multidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G70710-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 8 (EC:3.2.1.4)
Alternative name(s):
Cellulase 1
Short name:
AtCEL1
Endo-1,4-beta glucanase 8
Gene namesi
Name:CEL1
Ordered Locus Names:At1g70710
ORF Names:F5A18.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G70710.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 492468Endoglucanase 8PRO_0000249261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9CAC1.
PRIDEiQ9CAC1.

Expressioni

Tissue specificityi

Expressed in young expanding tissues. Expressed in xylem cells, young epidermal cells and newly formed cell walls.2 Publications

Gene expression databases

GenevestigatoriQ9CAC1.

Interactioni

Protein-protein interaction databases

MINTiMINT-8068205.

Structurei

3D structure databases

ProteinModelPortaliQ9CAC1.
SMRiQ9CAC1. Positions 28-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG128807.
HOGENOMiHOG000021033.
InParanoidiQ9CAC1.
OMAiEVLMGKA.
PhylomeDBiQ9CAC1.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CAC1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARKSLIFPV ILLAVLLFSP PIYSAGHDYR DALRKSILFF EGQRSGKLPP
60 70 80 90 100
DQRLKWRRDS ALRDGSSAGV DLSGGYYDAG DNIKFGFPMA FTTTMLSWSI
110 120 130 140 150
IDFGKTMGPE LRNAVKAVKW GTDYLLKATA IPGVVFVQVG DAYSDHNCWE
160 170 180 190 200
RPEDMDTLRT VYKIDRAHPG SDVAGETAAA LAAASIVFRK RDPAYSRLLL
210 220 230 240 250
DRATRVFAFA NRYRGAYSNS LYHAVCPFYC DFNGYQDELL WGAAWLHKAS
260 270 280 290 300
RKRAYREFIV KNEVILKAGD TINEFGWDNK HAGINVLISK EVLMGKAEYF
310 320 330 340 350
ESFKQNADGF ICSILPGISH PQVQYSRGGL LVKTGGSNMQ HVTSLSFLLL
360 370 380 390 400
AYSNYLSHAK KVVPCGELTA SPSLLRQIAK RQVDYILGDN PMGLSYMVGY
410 420 430 440 450
GQKFPRRIHH RGSSVPSVSA HPSHIGCKEG SRYFLSPNPN PNLLVGAVVG
460 470 480 490
GPNVTDAFPD SRPYFQQSEP TTYINAPLVG LLGYFSAHST WR
Length:492
Mass (Da):54,610
Last modified:June 1, 2001 - v1
Checksum:i7FDA753A52B97E88
GO

Sequence cautioni

The sequence CAA67156.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131L → H in CAA67157. (PubMed:9290636)Curated
Sequence conflicti71 – 711D → N in CAA67156. (PubMed:9290636)Curated
Sequence conflicti225 – 2251V → D in CAA67157. (PubMed:9290636)Curated
Sequence conflicti444 – 4441L → W in CAA67156. (PubMed:9290636)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98543 Genomic DNA. Translation: CAA67156.1. Sequence problems.
X98544 mRNA. Translation: CAA67157.1.
AC011663 Genomic DNA. Translation: AAG52329.1.
CP002684 Genomic DNA. Translation: AEE35103.1.
AY048283 mRNA. Translation: AAK82545.1.
AY074552 mRNA. Translation: AAL67092.1.
PIRiE96731.
RefSeqiNP_177228.1. NM_105739.3.
UniGeneiAt.21900.
At.72497.

Genome annotation databases

EnsemblPlantsiAT1G70710.1; AT1G70710.1; AT1G70710.
GeneIDi843408.
KEGGiath:AT1G70710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98543 Genomic DNA. Translation: CAA67156.1 . Sequence problems.
X98544 mRNA. Translation: CAA67157.1 .
AC011663 Genomic DNA. Translation: AAG52329.1 .
CP002684 Genomic DNA. Translation: AEE35103.1 .
AY048283 mRNA. Translation: AAK82545.1 .
AY074552 mRNA. Translation: AAL67092.1 .
PIRi E96731.
RefSeqi NP_177228.1. NM_105739.3.
UniGenei At.21900.
At.72497.

3D structure databases

ProteinModelPortali Q9CAC1.
SMRi Q9CAC1. Positions 28-476.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8068205.

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbi Q9CAC1.
PRIDEi Q9CAC1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G70710.1 ; AT1G70710.1 ; AT1G70710 .
GeneIDi 843408.
KEGGi ath:AT1G70710.

Organism-specific databases

TAIRi AT1G70710.

Phylogenomic databases

eggNOGi NOG128807.
HOGENOMi HOG000021033.
InParanoidi Q9CAC1.
OMAi EVLMGKA.
PhylomeDBi Q9CAC1.

Enzyme and pathway databases

BioCyci ARA:AT1G70710-MONOMER.

Gene expression databases

Genevestigatori Q9CAC1.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of elongation specific endo-1,4-beta-glucanase (cel1) from Arabidopsis thaliana."
    Shani Z., Dekel M., Tsabary G., Shoseyov O.
    Plant Mol. Biol. 34:837-842(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense."
    Tsabary G., Shani Z., Roiz L., Levy I., Riov J., Shoseyov O.
    Plant Mol. Biol. 51:213-224(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening."
    Shani Z., Dekel M., Roiz L., Horowitz M., Kolosovski N., Lapidot S., Alkan S., Koltai H., Tsabary G., Goren R., Shoseyov O.
    Plant Cell Rep. 25:1067-1074(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGUN8_ARATH
AccessioniPrimary (citable) accession number: Q9CAC1
Secondary accession number(s): O23696, O23697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3