Q9CAC1 (GUN8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 8
EC=3.2.1.4

Alternative name(s):
Cellulase 1
Short name=AtCEL1
Endo-1,4-beta glucanase 8

Gene names

Name:	CEL1
Ordered Locus Names:	At1g70710
ORF Names:	F5A18.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for cellulose formation of the cell wall. Ref.5
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in young expanding tissues. Expressed in xylem cells, young epidermal cells and newly formed cell walls. Ref.1 Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence CAA67156.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall modification involved in multidimensional cell growth Inferred from mutant phenotype Ref.5. Source: TAIR cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 492	468	Endoglucanase 8		PRO_0000249261
Sites
	Active site	409	1	By similarity
	Active site	460	1	By similarity
	Active site	469	1	By similarity
Amino acid modifications
	Glycosylation	453	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	13	1	L → H in CAA67157. Ref.1
	Sequence conflict	71	1	D → N in CAA67156. Ref.1
	Sequence conflict	225	1	V → D in CAA67157. Ref.1
	Sequence conflict	444	1	L → W in CAA67156. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9CAC1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7FDA753A52B97E88	FASTA	492	54,610
	10 20 30 40 50 60 MARKSLIFPV ILLAVLLFSP PIYSAGHDYR DALRKSILFF EGQRSGKLPP DQRLKWRRDS 70 80 90 100 110 120 ALRDGSSAGV DLSGGYYDAG DNIKFGFPMA FTTTMLSWSI IDFGKTMGPE LRNAVKAVKW 130 140 150 160 170 180 GTDYLLKATA IPGVVFVQVG DAYSDHNCWE RPEDMDTLRT VYKIDRAHPG SDVAGETAAA 190 200 210 220 230 240 LAAASIVFRK RDPAYSRLLL DRATRVFAFA NRYRGAYSNS LYHAVCPFYC DFNGYQDELL 250 260 270 280 290 300 WGAAWLHKAS RKRAYREFIV KNEVILKAGD TINEFGWDNK HAGINVLISK EVLMGKAEYF 310 320 330 340 350 360 ESFKQNADGF ICSILPGISH PQVQYSRGGL LVKTGGSNMQ HVTSLSFLLL AYSNYLSHAK 370 380 390 400 410 420 KVVPCGELTA SPSLLRQIAK RQVDYILGDN PMGLSYMVGY GQKFPRRIHH RGSSVPSVSA 430 440 450 460 470 480 HPSHIGCKEG SRYFLSPNPN PNLLVGAVVG GPNVTDAFPD SRPYFQQSEP TTYINAPLVG 490 LLGYFSAHST WR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of elongation specific endo-1,4-beta-glucanase (cel1) from Arabidopsis thaliana." Shani Z., Dekel M., Tsabary G., Shoseyov O. Plant Mol. Biol. 34:837-842(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense." Tsabary G., Shani Z., Roiz L., Levy I., Riov J., Shoseyov O. Plant Mol. Biol. 51:213-224(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[7]	"Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening." Shani Z., Dekel M., Roiz L., Horowitz M., Kolosovski N., Lapidot S., Alkan S., Koltai H., Tsabary G., Goren R., Shoseyov O. Plant Cell Rep. 25:1067-1074(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98543 Genomic DNA. Translation: CAA67156.1. Sequence problems. X98544 mRNA. Translation: CAA67157.1. AC011663 Genomic DNA. Translation: AAG52329.1. CP002684 Genomic DNA. Translation: AEE35103.1. AY048283 mRNA. Translation: AAK82545.1. AY074552 mRNA. Translation: AAL67092.1.
IPI	IPI00543752.
PIR	E96731.
RefSeq	NP_177228.1. NM_105739.3.
UniGene	At.21900. At.72497.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q9CAC1.
SMR	Q9CAC1. Positions 28-476.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q9CAC1.
PRIDE	Q9CAC1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G70710.1; AT1G70710.1; AT1G70710.
GeneID	843408.
KEGG	ath:AT1G70710.
Organism-specific databases
TAIR	At1g70710.
Phylogenomic databases
eggNOG	NOG128807.
HOGENOM	HOG000021033.
InParanoid	Q9CAC1.
OMA	INAPLMG.
PhylomeDB	Q9CAC1.
ProtClustDB	PLN02308.
Gene expression databases
Genevestigator	Q9CAC1.
GermOnline	AT1G70710. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN8_ARATH

Accession

Primary (citable) accession number: Q9CAC1
Secondary accession number(s): O23696, O23697

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program