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Q9CAC1

- GUN8_ARATH

UniProt

Q9CAC1 - GUN8_ARATH

Protein

Endoglucanase 8

Gene

CEL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Required for cellulose formation of the cell wall.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei409 – 4091By similarity
    Active sitei460 – 4601By similarity
    Active sitei469 – 4691By similarity

    GO - Molecular functioni

    1. cellulase activity Source: TAIR

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW
    2. cell wall modification involved in multidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciARA:AT1G70710-MONOMER.

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 8 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase 1
    Short name:
    AtCEL1
    Endo-1,4-beta glucanase 8
    Gene namesi
    Name:CEL1
    Ordered Locus Names:At1g70710
    ORF Names:F5A18.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G70710.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 492468Endoglucanase 8PRO_0000249261Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9CAC1.
    PRIDEiQ9CAC1.

    Expressioni

    Tissue specificityi

    Expressed in young expanding tissues. Expressed in xylem cells, young epidermal cells and newly formed cell walls.2 Publications

    Gene expression databases

    GenevestigatoriQ9CAC1.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8068205.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CAC1.
    SMRiQ9CAC1. Positions 28-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG128807.
    HOGENOMiHOG000021033.
    InParanoidiQ9CAC1.
    OMAiEVLMGKA.
    PhylomeDBiQ9CAC1.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9CAC1-1 [UniParc]FASTAAdd to Basket

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    MARKSLIFPV ILLAVLLFSP PIYSAGHDYR DALRKSILFF EGQRSGKLPP    50
    DQRLKWRRDS ALRDGSSAGV DLSGGYYDAG DNIKFGFPMA FTTTMLSWSI 100
    IDFGKTMGPE LRNAVKAVKW GTDYLLKATA IPGVVFVQVG DAYSDHNCWE 150
    RPEDMDTLRT VYKIDRAHPG SDVAGETAAA LAAASIVFRK RDPAYSRLLL 200
    DRATRVFAFA NRYRGAYSNS LYHAVCPFYC DFNGYQDELL WGAAWLHKAS 250
    RKRAYREFIV KNEVILKAGD TINEFGWDNK HAGINVLISK EVLMGKAEYF 300
    ESFKQNADGF ICSILPGISH PQVQYSRGGL LVKTGGSNMQ HVTSLSFLLL 350
    AYSNYLSHAK KVVPCGELTA SPSLLRQIAK RQVDYILGDN PMGLSYMVGY 400
    GQKFPRRIHH RGSSVPSVSA HPSHIGCKEG SRYFLSPNPN PNLLVGAVVG 450
    GPNVTDAFPD SRPYFQQSEP TTYINAPLVG LLGYFSAHST WR 492
    Length:492
    Mass (Da):54,610
    Last modified:June 1, 2001 - v1
    Checksum:i7FDA753A52B97E88
    GO

    Sequence cautioni

    The sequence CAA67156.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131L → H in CAA67157. (PubMed:9290636)Curated
    Sequence conflicti71 – 711D → N in CAA67156. (PubMed:9290636)Curated
    Sequence conflicti225 – 2251V → D in CAA67157. (PubMed:9290636)Curated
    Sequence conflicti444 – 4441L → W in CAA67156. (PubMed:9290636)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98543 Genomic DNA. Translation: CAA67156.1. Sequence problems.
    X98544 mRNA. Translation: CAA67157.1.
    AC011663 Genomic DNA. Translation: AAG52329.1.
    CP002684 Genomic DNA. Translation: AEE35103.1.
    AY048283 mRNA. Translation: AAK82545.1.
    AY074552 mRNA. Translation: AAL67092.1.
    PIRiE96731.
    RefSeqiNP_177228.1. NM_105739.3.
    UniGeneiAt.21900.
    At.72497.

    Genome annotation databases

    EnsemblPlantsiAT1G70710.1; AT1G70710.1; AT1G70710.
    GeneIDi843408.
    KEGGiath:AT1G70710.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98543 Genomic DNA. Translation: CAA67156.1 . Sequence problems.
    X98544 mRNA. Translation: CAA67157.1 .
    AC011663 Genomic DNA. Translation: AAG52329.1 .
    CP002684 Genomic DNA. Translation: AEE35103.1 .
    AY048283 mRNA. Translation: AAK82545.1 .
    AY074552 mRNA. Translation: AAL67092.1 .
    PIRi E96731.
    RefSeqi NP_177228.1. NM_105739.3.
    UniGenei At.21900.
    At.72497.

    3D structure databases

    ProteinModelPortali Q9CAC1.
    SMRi Q9CAC1. Positions 28-476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8068205.

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Proteomic databases

    PaxDbi Q9CAC1.
    PRIDEi Q9CAC1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G70710.1 ; AT1G70710.1 ; AT1G70710 .
    GeneIDi 843408.
    KEGGi ath:AT1G70710.

    Organism-specific databases

    TAIRi AT1G70710.

    Phylogenomic databases

    eggNOGi NOG128807.
    HOGENOMi HOG000021033.
    InParanoidi Q9CAC1.
    OMAi EVLMGKA.
    PhylomeDBi Q9CAC1.

    Enzyme and pathway databases

    BioCyci ARA:AT1G70710-MONOMER.

    Gene expression databases

    Genevestigatori Q9CAC1.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of elongation specific endo-1,4-beta-glucanase (cel1) from Arabidopsis thaliana."
      Shani Z., Dekel M., Tsabary G., Shoseyov O.
      Plant Mol. Biol. 34:837-842(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense."
      Tsabary G., Shani Z., Roiz L., Levy I., Riov J., Shoseyov O.
      Plant Mol. Biol. 51:213-224(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
      Libertini E., Li Y., McQueen-Mason S.J.
      J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    7. "Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening."
      Shani Z., Dekel M., Roiz L., Horowitz M., Kolosovski N., Lapidot S., Alkan S., Koltai H., Tsabary G., Goren R., Shoseyov O.
      Plant Cell Rep. 25:1067-1074(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGUN8_ARATH
    AccessioniPrimary (citable) accession number: Q9CAC1
    Secondary accession number(s): O23696, O23697
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3