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Q9CAC1 (GUN8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 8

EC=3.2.1.4
Alternative name(s):
Cellulase 1
Short name=AtCEL1
Endo-1,4-beta glucanase 8
Gene names
Name:CEL1
Ordered Locus Names:At1g70710
ORF Names:F5A18.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for cellulose formation of the cell wall. Ref.5

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in young expanding tissues. Expressed in xylem cells, young epidermal cells and newly formed cell walls. Ref.1 Ref.7

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Sequence caution

The sequence CAA67156.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 492468Endoglucanase 8
PRO_0000249261

Sites

Active site4091 By similarity
Active site4601 By similarity
Active site4691 By similarity

Amino acid modifications

Glycosylation4531N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict131L → H in CAA67157. Ref.1
Sequence conflict711D → N in CAA67156. Ref.1
Sequence conflict2251V → D in CAA67157. Ref.1
Sequence conflict4441L → W in CAA67156. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9CAC1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7FDA753A52B97E88

FASTA49254,610
        10         20         30         40         50         60 
MARKSLIFPV ILLAVLLFSP PIYSAGHDYR DALRKSILFF EGQRSGKLPP DQRLKWRRDS 

        70         80         90        100        110        120 
ALRDGSSAGV DLSGGYYDAG DNIKFGFPMA FTTTMLSWSI IDFGKTMGPE LRNAVKAVKW 

       130        140        150        160        170        180 
GTDYLLKATA IPGVVFVQVG DAYSDHNCWE RPEDMDTLRT VYKIDRAHPG SDVAGETAAA 

       190        200        210        220        230        240 
LAAASIVFRK RDPAYSRLLL DRATRVFAFA NRYRGAYSNS LYHAVCPFYC DFNGYQDELL 

       250        260        270        280        290        300 
WGAAWLHKAS RKRAYREFIV KNEVILKAGD TINEFGWDNK HAGINVLISK EVLMGKAEYF 

       310        320        330        340        350        360 
ESFKQNADGF ICSILPGISH PQVQYSRGGL LVKTGGSNMQ HVTSLSFLLL AYSNYLSHAK 

       370        380        390        400        410        420 
KVVPCGELTA SPSLLRQIAK RQVDYILGDN PMGLSYMVGY GQKFPRRIHH RGSSVPSVSA 

       430        440        450        460        470        480 
HPSHIGCKEG SRYFLSPNPN PNLLVGAVVG GPNVTDAFPD SRPYFQQSEP TTYINAPLVG 

       490 
LLGYFSAHST WR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of elongation specific endo-1,4-beta-glucanase (cel1) from Arabidopsis thaliana."
Shani Z., Dekel M., Tsabary G., Shoseyov O.
Plant Mol. Biol. 34:837-842(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense."
Tsabary G., Shani Z., Roiz L., Levy I., Riov J., Shoseyov O.
Plant Mol. Biol. 51:213-224(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"Expression of endo-1,4-beta-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening."
Shani Z., Dekel M., Roiz L., Horowitz M., Kolosovski N., Lapidot S., Alkan S., Koltai H., Tsabary G., Goren R., Shoseyov O.
Plant Cell Rep. 25:1067-1074(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98543 Genomic DNA. Translation: CAA67156.1. Sequence problems.
X98544 mRNA. Translation: CAA67157.1.
AC011663 Genomic DNA. Translation: AAG52329.1.
CP002684 Genomic DNA. Translation: AEE35103.1.
AY048283 mRNA. Translation: AAK82545.1.
AY074552 mRNA. Translation: AAL67092.1.
PIRE96731.
RefSeqNP_177228.1. NM_105739.3.
UniGeneAt.21900.
At.72497.

3D structure databases

ProteinModelPortalQ9CAC1.
SMRQ9CAC1. Positions 28-476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8068205.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbQ9CAC1.
PRIDEQ9CAC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G70710.1; AT1G70710.1; AT1G70710.
GeneID843408.
KEGGath:AT1G70710.

Organism-specific databases

TAIRAT1G70710.

Phylogenomic databases

eggNOGNOG128807.
HOGENOMHOG000021033.
InParanoidQ9CAC1.
OMAEVLMGKA.
PhylomeDBQ9CAC1.

Enzyme and pathway databases

BioCycARA:AT1G70710-MONOMER.

Gene expression databases

GenevestigatorQ9CAC1.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN8_ARATH
AccessionPrimary (citable) accession number: Q9CAC1
Secondary accession number(s): O23696, O23697
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names