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Protein

Glyoxylate/hydroxypyruvate reductase A HPR2

Gene

HPR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate (HP) into glycolate and glycerate in the cytoplasm, thus providing a cytosolic bypass to the photorespiratory core cycle. Mostly active in the presence of NADPH and hydroxypyruvate.2 Publications

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.1 Publication
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.1 Publication

Enzyme regulationi

Strongly inhibited by oxalate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232By similarity1
Binding sitei256NADPBy similarity1
Active sitei261By similarity1
Active sitei279Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 155NADPBy similarity4
Nucleotide bindingi174 – 176NADPBy similarity3
Nucleotide bindingi230 – 232NADPBy similarity3
Nucleotide bindingi279 – 281NADPBy similarity3

GO - Molecular functioni

  • glyoxylate reductase (NADP) activity Source: UniProtKB
  • hydroxypyruvate reductase activity Source: UniProtKB
  • NAD binding Source: InterPro

GO - Biological processi

  • oxidative photosynthetic carbon pathway Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolate pathway, Photorespiration
LigandNAD, NADP, Pyruvate

Enzyme and pathway databases

BioCyciARA:AT1G79870-MONOMER
BRENDAi1.1.1.81 399
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate/hydroxypyruvate reductase A HPR2 (EC:1.1.1.79, EC:1.1.1.81)
Alternative name(s):
NAD(P)H-dependent hydroxypyruvate reductase 2
Short name:
AtHPR2
Short name:
HPR 2
Gene namesi
Name:HPR2
Ordered Locus Names:At1g79870
ORF Names:F19K16.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G79870
TAIRilocus:2017824 AT1G79870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Elevated levels of hydroxypyruvate and other metabolites in leaves. Under long-day conditions, slightly altered photosynthetic gas exchange. When associated with HPR1 disruption, strong air-sensitivity and dramatic reduction in photosynthetic performance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004199521 – 313Glyoxylate/hydroxypyruvate reductase A HPR2Add BLAST313

Proteomic databases

PaxDbiQ9CA90
PRIDEiQ9CA90

PTM databases

iPTMnetiQ9CA90

Expressioni

Gene expression databases

ExpressionAtlasiQ9CA90 baseline and differential
GenevisibleiQ9CA90 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G79870.1

Structurei

3D structure databases

ProteinModelPortaliQ9CA90
SMRiQ9CA90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0069 Eukaryota
COG1052 LUCA
HOGENOMiHOG000136700
InParanoidiQ9CA90
KOiK15919
OMAiKWIAHNG
OrthoDBiEOG09360H09
PhylomeDBiQ9CA90

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029752 D-isomer_DH_CS1
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00065 D_2_HYDROXYACID_DH_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CA90-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESIGVLMMC PMSSYLENEL EKRFNLLRFW TSPEKSVLLE THRNSIRAVV
60 70 80 90 100
GNASAGADAQ LISDLPNLEI VSSFSVGLDK IDLGKCKEKG IRVTNTPDVL
110 120 130 140 150
TEDVADLAIG LILALLRRLC ECDRYVRSGK WKQGEFQLTT KFSGKSVGII
160 170 180 190 200
GLGRIGTAIA KRAEAFSCPI NYYSRTIKPD VAYKYYPTVV DLAQNSDILV
210 220 230 240 250
VACPLTEQTR HIVDRQVMDA LGAKGVLINI GRGPHVDEQE LIKALTEGRL
260 270 280 290 300
GGAALDVFEQ EPHVPEELFG LENVVLLPHV GSGTVETRNA MADLVVGNLE
310
AHFSGKSLLT PVV
Length:313
Mass (Da):34,161
Last modified:June 1, 2001 - v1
Checksum:iA8D52C698A03B183
GO
Isoform 2 (identifier: Q9CA90-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-152: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:294
Mass (Da):32,197
Checksum:i55F17371E075DB34
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044377134 – 152Missing in isoform 2. CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011717 Genomic DNA Translation: AAG52259.1
CP002684 Genomic DNA Translation: AEE36315.1
CP002684 Genomic DNA Translation: AEE36316.1
AY069901 mRNA Translation: AAL47452.1
AY113022 mRNA Translation: AAM47330.1
AY088166 mRNA Translation: AAM65710.1
PIRiG96829
RefSeqiNP_001185444.1, NM_001198515.1 [Q9CA90-2]
NP_178105.1, NM_106636.3 [Q9CA90-1]
UniGeneiAt.27554

Genome annotation databases

EnsemblPlantsiAT1G79870.1; AT1G79870.1; AT1G79870 [Q9CA90-1]
AT1G79870.2; AT1G79870.2; AT1G79870 [Q9CA90-2]
GeneIDi844326
GrameneiAT1G79870.1; AT1G79870.1; AT1G79870 [Q9CA90-1]
AT1G79870.2; AT1G79870.2; AT1G79870 [Q9CA90-2]
KEGGiath:AT1G79870

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHPR2_ARATH
AccessioniPrimary (citable) accession number: Q9CA90
Secondary accession number(s): F4HQC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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