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Protein

Adagio protein 3

Gene

ADO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulates 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.8 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • core promoter binding Source: TAIR
  • photoreceptor activity Source: UniProtKB-KW

GO - Biological processi

  • circadian rhythm Source: TAIR
  • flower development Source: UniProtKB-KW
  • positive regulation of flower development Source: TAIR
  • protein-chromophore linkage Source: UniProtKB-KW
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of gene expression Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to blue light Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Biological rhythms, Flowering, Sensory transduction, Ubl conjugation pathway

Keywords - Ligandi

Chromophore, Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Adagio protein 3
Alternative name(s):
F-box only protein 2a
Short name:
FBX2a
Flavin-binding kelch repeat F-box protein 1
Gene namesi
Name:ADO3
Synonyms:FKF1
Ordered Locus Names:At1g68050
ORF Names:T23K23.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G68050.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Moves from the nucleus into cytosolic speckles upon interaction with ADO1 and ADO2.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Late flowering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91C → A: No FMN binding and decreased interaction with GI. 2 Publications1
Mutagenesisi92R → D: Decreased interaction with GI. 1 Publication1
Mutagenesisi163Q → L: Decreased interaction with GI. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199581 – 619Adagio protein 3Add BLAST619

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91S-4a-FMN cysteine1

Post-translational modificationi

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark.

Proteomic databases

PaxDbiQ9C9W9.

PTM databases

iPTMnetiQ9C9W9.

Expressioni

Tissue specificityi

Highly expressed in stomata and leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments.2 Publications

Inductioni

Circadian-regulation. Expression is higher during the late light phase than during the dark phase.3 Publications

Gene expression databases

GenevisibleiQ9C9W9. AT.

Interactioni

Subunit structurei

Interacts with ADO1 (via Kelch repeats), ADO2 (via Kelch repeats), SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14. Interacts (via Kelch repeats) with CDF1, CDF2 and CDF3. Interacts (via N-terminus) with CO and GI (via N-terminus) in a blue-light-dependent manner.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDF1Q8W1E32EBI-401228,EBI-1536051
CDF2Q93ZL52EBI-401228,EBI-1536103
CDF3Q8LFV32EBI-401228,EBI-1536119
GIQ9SQI210EBI-401228,EBI-446380
SKP1BQ9FHW75EBI-401228,EBI-604076
SRK2GP432923EBI-401228,EBI-401174

Protein-protein interaction databases

BioGridi28354. 22 interactors.
DIPiDIP-31333N.
IntActiQ9C9W9. 16 interactors.
STRINGi3702.AT1G68050.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C9W9.
SMRiQ9C9W9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 123PASPROSITE-ProRule annotationAdd BLAST80
Domaini127 – 168PACAdd BLAST42
Domaini211 – 257F-boxAdd BLAST47
Repeati304 – 354Kelch 1Add BLAST51
Repeati357 – 404Kelch 2Add BLAST48
Repeati409 – 457Kelch 3Add BLAST49
Repeati462 – 513Kelch 4Add BLAST52
Repeati523 – 571Kelch 5Add BLAST49

Sequence similaritiesi

Belongs to the ADAGIO family.Curated
Contains 1 F-box domain.Curated
Contains 5 Kelch repeats.Curated
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IKJW. Eukaryota.
ENOG410XRGE. LUCA.
HOGENOMiHOG000238789.
InParanoidiQ9C9W9.
KOiK12116.
OMAiKQQSNHR.
OrthoDBiEOG093604YP.
PhylomeDBiQ9C9W9.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR001810. F-box_dom.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR001610. PAC.
IPR000014. PAS.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF81383. SSF81383. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C9W9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAREHAIGEA TGKRKKRGRV EEAEEYCNDG IEEQVEDEKL PLEVGMFYYP
60 70 80 90 100
MTPPSFIVSD ALEPDFPLIY VNRVFEVFTG YRADEVLGRN CRFLQYRDPR
110 120 130 140 150
AQRRHPLVDP VVVSEIRRCL EEGIEFQGEL LNFRKDGTPL VNRLRLAPIR
160 170 180 190 200
DDDGTITHVI GIQVFSETTI DLDRVSYPVF KHKQQLDQTS ECLFPSGSPR
210 220 230 240 250
FKEHHEDFCG ILQLSDEVLA HNILSRLTPR DVASIGSACR RLRQLTKNES
260 270 280 290 300
VRKMVCQNAW GKEITGTLEI MTKKLRWGRL ARELTTLEAV CWRKFTVGGI
310 320 330 340 350
VQPSRCNFSA CAVGNRLVLF GGEGVNMQPL DDTFVLNLDA ECPEWQRVRV
360 370 380 390 400
TSSPPGRWGH TLSCLNGSWL VVFGGCGRQG LLNDVFVLDL DAKHPTWKEV
410 420 430 440 450
AGGTPPLPRS WHSSCTIEGS KLVVSGGCTD AGVLLSDTFL LDLTTDKPTW
460 470 480 490 500
KEIPTSWAPP SRLGHSLSVF GRTKILMFGG LANSGHLKLR SGEAYTIDLE
510 520 530 540 550
DEEPRWRELE CSAFPGVVVP PPRLDHVAVS MPCGRVIIFG GSIAGLHSPS
560 570 580 590 600
QLFLIDPAEE KPSWRILNVP GKPPKLAWGH STCVVGGTRV LVLGGHTGEE
610
WILNELHELC LASRQDSDL
Length:619
Mass (Da):69,063
Last modified:June 1, 2001 - v1
Checksum:iAC90C0641149A3D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti484S → I in AAF32298 (PubMed:10847687).Curated1
Sequence conflicti484S → I in AAK27435 (PubMed:11260718).Curated1
Sequence conflicti581S → N in AAF32298 (PubMed:10847687).Curated1
Sequence conflicti581S → N in AAK27435 (PubMed:11260718).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216523 mRNA. Translation: AAF32298.2.
AF252296 mRNA. Translation: AAK27435.1.
AC012563 Genomic DNA. Translation: AAG51994.1.
CP002684 Genomic DNA. Translation: AEE34741.1.
AY064999 mRNA. Translation: AAL57647.1.
AY113029 mRNA. Translation: AAM47337.1.
PIRiF96703.
RefSeqiNP_564919.1. NM_105475.4.
UniGeneiAt.20568.

Genome annotation databases

EnsemblPlantsiAT1G68050.1; AT1G68050.1; AT1G68050.
GeneIDi843133.
GrameneiAT1G68050.1; AT1G68050.1; AT1G68050.
KEGGiath:AT1G68050.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216523 mRNA. Translation: AAF32298.2.
AF252296 mRNA. Translation: AAK27435.1.
AC012563 Genomic DNA. Translation: AAG51994.1.
CP002684 Genomic DNA. Translation: AEE34741.1.
AY064999 mRNA. Translation: AAL57647.1.
AY113029 mRNA. Translation: AAM47337.1.
PIRiF96703.
RefSeqiNP_564919.1. NM_105475.4.
UniGeneiAt.20568.

3D structure databases

ProteinModelPortaliQ9C9W9.
SMRiQ9C9W9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28354. 22 interactors.
DIPiDIP-31333N.
IntActiQ9C9W9. 16 interactors.
STRINGi3702.AT1G68050.1.

PTM databases

iPTMnetiQ9C9W9.

Proteomic databases

PaxDbiQ9C9W9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G68050.1; AT1G68050.1; AT1G68050.
GeneIDi843133.
GrameneiAT1G68050.1; AT1G68050.1; AT1G68050.
KEGGiath:AT1G68050.

Organism-specific databases

TAIRiAT1G68050.

Phylogenomic databases

eggNOGiENOG410IKJW. Eukaryota.
ENOG410XRGE. LUCA.
HOGENOMiHOG000238789.
InParanoidiQ9C9W9.
KOiK12116.
OMAiKQQSNHR.
OrthoDBiEOG093604YP.
PhylomeDBiQ9C9W9.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9C9W9.

Gene expression databases

GenevisibleiQ9C9W9. AT.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR001810. F-box_dom.
IPR015915. Kelch-typ_b-propeller.
IPR011498. Kelch_2.
IPR001610. PAC.
IPR000014. PAS.
[Graphical view]
PfamiPF07646. Kelch_2. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF81383. SSF81383. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADO3_ARATH
AccessioniPrimary (citable) accession number: Q9C9W9
Secondary accession number(s): Q9M648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Adagio' means slowly in Italian.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.