Reviewed, UniProtKB/Swiss-Prot Q9C9W9 (ADO3_ARATH)

Names and origin

Protein names

Recommended name:
    Adagio protein 3
Alternative name(s):
    Flavin-binding kelch repeat F-box protein 1
    F-box only protein 2a
      Short name=FBX2a

Gene names

Name:	ADO3
Synonyms:	FKF1
Ordered Locus Names:	At1g68050
ORF Names:	T23K23.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. Ref.1 Ref.6 Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with ADO2, SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14.
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments. Ref.1
Induction	Circadian-regulation. Expression is higher during the light phase than during the dark phase. Ref.1 Ref.6
Post-translational modification	FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark.
Miscellaneous	'Adagio' means slowly in Italian.
Sequence similarities	Belongs to the ADAGIO family. Contains 1 F-box domain. Contains 5 Kelch repeats. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Biological rhythms Sensory transduction Ubl conjugation pathway
Cellular component	Nucleus
Domain	Kelch repeat Repeat
Ligand	Chromophore FMN Flavoprotein
Molecular function	Photoreceptor protein Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	circadian rhythm Inferred from direct assay. Source: TAIR modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of flower development Ref.1 Inferred from mutant phenotype. Source: TAIR protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to blue light Inferred from direct assay. Source: TAIR signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.8 Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AT4g34210	O49484	2	EBI-401228,EBI-401185
DOF3.3	Q8LFV3	1	EBI-401228,EBI-1536119
DOF5.2	Q93ZL5	1	EBI-401228,EBI-1536103
DOF5.5	Q8W1E3	1	EBI-401228,EBI-1536051
GI	Q9SQI2	10	EBI-401228,EBI-446380
SKP1B	Q9FHW7	2	EBI-401228,EBI-604076
SRK2A	P43291	2	EBI-401228,EBI-401164
SRK2G	P43292	2	EBI-401228,EBI-401174

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Adagio protein 3		PRO_0000119958
Regions
	Domain	44 – 123	80	PAS
	Domain	127 – 168	42	PAC
	Domain	211 – 257	47	F-box
	Repeat	304 – 354	51	Kelch 1
	Repeat	357 – 404	48	Kelch 2
	Repeat	409 – 457	49	Kelch 3
	Repeat	462 – 513	52	Kelch 4
	Repeat	523 – 571	49	Kelch 5
Amino acid modifications
	Modified residue	91	1	S-4a-FMN cysteine
Experimental info
	Mutagenesis	91	1	C → A: No FMN binding. Ref.6
	Sequence conflict	484	1	S → I Ref.1
	Sequence conflict	484	1	S → I Ref.2
	Sequence conflict	581	1	S → N Ref.1
	Sequence conflict	581	1	S → N Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9C9W9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: AC90C0641149A3D8	FASTA	619	69,063
	10 20 30 40 50 60 MAREHAIGEA TGKRKKRGRV EEAEEYCNDG IEEQVEDEKL PLEVGMFYYP MTPPSFIVSD 70 80 90 100 110 120 ALEPDFPLIY VNRVFEVFTG YRADEVLGRN CRFLQYRDPR AQRRHPLVDP VVVSEIRRCL 130 140 150 160 170 180 EEGIEFQGEL LNFRKDGTPL VNRLRLAPIR DDDGTITHVI GIQVFSETTI DLDRVSYPVF 190 200 210 220 230 240 KHKQQLDQTS ECLFPSGSPR FKEHHEDFCG ILQLSDEVLA HNILSRLTPR DVASIGSACR 250 260 270 280 290 300 RLRQLTKNES VRKMVCQNAW GKEITGTLEI MTKKLRWGRL ARELTTLEAV CWRKFTVGGI 310 320 330 340 350 360 VQPSRCNFSA CAVGNRLVLF GGEGVNMQPL DDTFVLNLDA ECPEWQRVRV TSSPPGRWGH 370 380 390 400 410 420 TLSCLNGSWL VVFGGCGRQG LLNDVFVLDL DAKHPTWKEV AGGTPPLPRS WHSSCTIEGS 430 440 450 460 470 480 KLVVSGGCTD AGVLLSDTFL LDLTTDKPTW KEIPTSWAPP SRLGHSLSVF GRTKILMFGG 490 500 510 520 530 540 LANSGHLKLR SGEAYTIDLE DEEPRWRELE CSAFPGVVVP PPRLDHVAVS MPCGRVIIFG 550 560 570 580 590 600 GSIAGLHSPS QLFLIDPAEE KPSWRILNVP GKPPKLAWGH STCVVGGTRV LVLGGHTGEE 610 WILNELHELC LASRQDSDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis." Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B. Cell 101:331-340(2000) [PubMed: 10847687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Landsberg erecta.
[2]	"An Arabidopsis circadian clock component interacts with both CRY1 and phyB." Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R. Nature 410:487-490(2001) [PubMed: 11260718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis." Imaizumi T., Tran H.G., Swartz T.E., Briggs W.R., Kay S.A. Nature 426:302-306(2003) [PubMed: 14628054] [Abstract] Cited for: FUNCTION, INDUCTION, FMN-BINDING AT CYS-91, MUTAGENESIS OF CYS-91.
[7]	"Functional conservation of light, oxygen, or voltage domains in light sensing." Cheng P., He Q., Yang Y., Wang L., Liu Y. Proc. Natl. Acad. Sci. U.S.A. 100:5938-5943(2003) [PubMed: 12719523] [Abstract] Cited for: FUNCTION.
[8]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1K; SKP1N AND ADO2.
[9]	"Expression and interaction analysis of Arabidopsis Skp1-related genes." Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M. Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK3; ASK11; ASK12 AND ASK13.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF216523 mRNA. Translation: AAF32298.2. AF252296 mRNA. Translation: AAK27435.1. AC012563 Genomic DNA. Translation: AAG51994.1. AY064999 mRNA. Translation: AAL57647.1. AY113029 mRNA. Translation: AAM47337.1.
IPI	IPI00537296.
PIR	F96703.
RefSeq	NP_564919.1.
UniGene	At.20568
3D structure databases
HSSP	HSSP built from PDB template 1N9L based on UniProtKB Q8LPE0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C9W9. 16 interactions.
Genome annotation databases
GeneID	843133.
GenomeReviews	Gene locus AT1G68050 in contig CT485782_GR.
KEGG	ath:AT1G68050.
NMPDR	fig|3702.1.peg.6256.
Organism-specific databases
GeneFarm	5108. 485.
TAIR	At1g68050.
Phylogenomic databases
OMA	Q9C9W9. GRTKILM.
Gene expression databases
GermOnline	AT1G68050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR001610. PAC. IPR000014. PAS. IPR013655. PAS_fold_3. [Graphical view]
Gene3D	G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit.
Pfam	PF00646. F-box. 1 hit. PF01344. Kelch_1. 2 hits. PF07646. Kelch_2. 3 hits. PF08447. PAS_3. 1 hit. [Graphical view]
SMART	SM00086. PAC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50181. FBOX. False negative. PS50113. PAC. False negative. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADO3_ARATH

Accession

Primary (citable) accession number: Q9C9W9
Secondary accession number(s): Q9M648

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)