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Q9C9W9 (ADO3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adagio protein 3
Alternative name(s):
F-box only protein 2a
Short name=FBX2a
Flavin-binding kelch repeat F-box protein 1
Gene names
Name:ADO3
Synonyms:FKF1
Ordered Locus Names:At1g68050
ORF Names:T23K23.10
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. Ref.1 Ref.7 Ref.8

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with ADO2, SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14. Ref.9 Ref.10

Subcellular location

Nucleus By similarity.

Tissue specificity

Highly expressed in leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments. Ref.1

Induction

Circadian-regulation. Expression is higher during the light phase than during the dark phase. Ref.1 Ref.7

Post-translational modification

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark.

Miscellaneous

'Adagio' means slowly in Italian.

Sequence similarities

Belongs to the ADAGIO family.

Contains 1 F-box domain.

Contains 5 Kelch repeats.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Adagio protein 3
PRO_0000119958

Regions

Domain44 – 12380PAS
Domain127 – 16842PAC
Domain211 – 25747F-box
Repeat304 – 35451Kelch 1
Repeat357 – 40448Kelch 2
Repeat409 – 45749Kelch 3
Repeat462 – 51352Kelch 4
Repeat523 – 57149Kelch 5

Amino acid modifications

Modified residue911S-4a-FMN cysteine

Experimental info

Mutagenesis911C → A: No FMN binding. Ref.7
Sequence conflict4841S → I in AAF32298. Ref.1
Sequence conflict4841S → I in AAK27435. Ref.2
Sequence conflict5811S → N in AAF32298. Ref.1
Sequence conflict5811S → N in AAK27435. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9C9W9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: AC90C0641149A3D8

FASTA61969,063
        10         20         30         40         50         60 
MAREHAIGEA TGKRKKRGRV EEAEEYCNDG IEEQVEDEKL PLEVGMFYYP MTPPSFIVSD 

        70         80         90        100        110        120 
ALEPDFPLIY VNRVFEVFTG YRADEVLGRN CRFLQYRDPR AQRRHPLVDP VVVSEIRRCL 

       130        140        150        160        170        180 
EEGIEFQGEL LNFRKDGTPL VNRLRLAPIR DDDGTITHVI GIQVFSETTI DLDRVSYPVF 

       190        200        210        220        230        240 
KHKQQLDQTS ECLFPSGSPR FKEHHEDFCG ILQLSDEVLA HNILSRLTPR DVASIGSACR 

       250        260        270        280        290        300 
RLRQLTKNES VRKMVCQNAW GKEITGTLEI MTKKLRWGRL ARELTTLEAV CWRKFTVGGI 

       310        320        330        340        350        360 
VQPSRCNFSA CAVGNRLVLF GGEGVNMQPL DDTFVLNLDA ECPEWQRVRV TSSPPGRWGH 

       370        380        390        400        410        420 
TLSCLNGSWL VVFGGCGRQG LLNDVFVLDL DAKHPTWKEV AGGTPPLPRS WHSSCTIEGS 

       430        440        450        460        470        480 
KLVVSGGCTD AGVLLSDTFL LDLTTDKPTW KEIPTSWAPP SRLGHSLSVF GRTKILMFGG 

       490        500        510        520        530        540 
LANSGHLKLR SGEAYTIDLE DEEPRWRELE CSAFPGVVVP PPRLDHVAVS MPCGRVIIFG 

       550        560        570        580        590        600 
GSIAGLHSPS QLFLIDPAEE KPSWRILNVP GKPPKLAWGH STCVVGGTRV LVLGGHTGEE 

       610 
WILNELHELC LASRQDSDL

« Hide

References

« Hide 'large scale' references
[1]"FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis."
Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B.
Cell 101:331-340(2000) [PubMed: 10847687] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Landsberg erecta.
[2]"An Arabidopsis circadian clock component interacts with both CRY1 and phyB."
Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R.
Nature 410:487-490(2001) [PubMed: 11260718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"F-box proteins in Arabidopsis."
Xiao W., Jang J.-C.
Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[7]"FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis."
Imaizumi T., Tran H.G., Swartz T.E., Briggs W.R., Kay S.A.
Nature 426:302-306(2003) [PubMed: 14628054] [Abstract]
Cited for: FUNCTION, INDUCTION, FMN-BINDING AT CYS-91, MUTAGENESIS OF CYS-91.
[8]"Functional conservation of light, oxygen, or voltage domains in light sensing."
Cheng P., He Q., Yang Y., Wang L., Liu Y.
Proc. Natl. Acad. Sci. U.S.A. 100:5938-5943(2003) [PubMed: 12719523] [Abstract]
Cited for: FUNCTION.
[9]"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract]
Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1K; SKP1N AND ADO2.
[10]"Expression and interaction analysis of Arabidopsis Skp1-related genes."
Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M.
Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract]
Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK3; ASK11; ASK12 AND ASK13.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF216523 mRNA. Translation: AAF32298.2.
AF252296 mRNA. Translation: AAK27435.1.
AC012563 Genomic DNA. Translation: AAG51994.1.
CP002684 Genomic DNA. Translation: AEE34741.1.
AY064999 mRNA. Translation: AAL57647.1.
AY113029 mRNA. Translation: AAM47337.1.
IPIIPI00537296.
PIRF96703.
RefSeqNP_564919.1. NM_105475.3.
UniGeneAt.20568.

3D structure databases

ProteinModelPortalQ9C9W9.
SMRQ9C9W9. Positions 50-168, 211-255, 284-471.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9C9W9. 16 interactions.
STRINGQ9C9W9.

Proteomic databases

PRIDEQ9C9W9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G68050.1; AT1G68050.1; AT1G68050.
GeneID843133.
GenomeReviewsGene locus AT1G68050 in contig CT485782_GR.
KEGGath:AT1G68050.
NMPDRfig|3702.1.peg.6256.

Organism-specific databases

GeneFarm5108. 485.
TAIRAt1g68050.

Phylogenomic databases

eggNOGeuNOG04723.
GeneTreeEPGT00070000028386.
HOGENOMHBG319046.
InParanoidQ9C9W9.
OMAGRTKILM.
PhylomeDBQ9C9W9.
ProtClustDBCLSN2689078.

Gene expression databases

GenevestigatorQ9C9W9.
GermOnlineAT1G68050. Arabidopsis thaliana.

Family and domain databases

InterProIPR022364. F-box_dom_Skp2-like.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR011498. Kelch_2.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
Gene3DG3DSA:2.120.10.80. Kelch-typ_b-propeller. 2 hits.
KOK12116.
PfamPF01344. Kelch_1. 1 hit.
PF07646. Kelch_2. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
SMARTSM00086. PAC. 1 hit.
[Graphical view]
SUPFAMSSF81383. F-box_dom_Skp2-like. 1 hit.
TIGRFAMsTIGR00229. Sensory_box. 1 hit.
PROSITEPS50181. FBOX. False negative.
PS50113. PAC. False negative.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADO3_ARATH
AccessionPrimary (citable) accession number: Q9C9W9
Secondary accession number(s): Q9M648
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 1, 2001
Last modified: December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents