Reviewed,
UniProtKB/Swiss-Prot Q9C9W9 (ADO3_ARATH)
Last modified
June 16, 2009.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Adagio protein 3 Alternative name(s): Flavin-binding kelch repeat F-box protein 1 F-box only protein 2a Short name=FBX2a | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 619 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of E3 ubiquitin ligase complexes that plays a central role blue-light-dependent circadian cycles. Acts as a blue-light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaves movement rhythms. Ref.1 Ref.6 Ref.7 |
| Pathway | |
| Subunit structure | Interacts with ADO2, SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14. |
| Subcellular location | Nucleus By similarity. |
| Tissue specificity | Highly expressed in leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments. Ref.1 |
| Induction | Circadian-regulation. Expression is higher during the light phase than during the dark phase. Ref.1 Ref.6 |
| Post-translational modification | FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark. |
| Miscellaneous | 'Adagio' means slowly in Italian. |
| Sequence similarities | Belongs to the ADAGIO family. Contains 1 F-box domain. Contains 5 Kelch repeats. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| AT4g34210 | O49484 | 2 | EBI-401228,EBI-401185 | |
| DOF3.3 | Q8LFV3 | 1 | EBI-401228,EBI-1536119 | |
| DOF5.2 | Q93ZL5 | 1 | EBI-401228,EBI-1536103 | |
| DOF5.5 | Q8W1E3 | 1 | EBI-401228,EBI-1536051 | |
| GI | Q9SQI2 | 10 | EBI-401228,EBI-446380 | |
| SKP1B | Q9FHW7 | 2 | EBI-401228,EBI-604076 | |
| SRK2A | P43291 | 2 | EBI-401228,EBI-401164 | |
| SRK2G | P43292 | 2 | EBI-401228,EBI-401174 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 619 | 619 | Adagio protein 3 | PRO_0000119958 | |||||
Regions | |||||||||
| Domain | 44 – 123 | 80 | PAS | ||||||
| Domain | 127 – 168 | 42 | PAC | ||||||
| Domain | 211 – 257 | 47 | F-box | ||||||
| Repeat | 304 – 354 | 51 | Kelch 1 | ||||||
| Repeat | 357 – 404 | 48 | Kelch 2 | ||||||
| Repeat | 409 – 457 | 49 | Kelch 3 | ||||||
| Repeat | 462 – 513 | 52 | Kelch 4 | ||||||
| Repeat | 523 – 571 | 49 | Kelch 5 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 91 | 1 | S-4a-FMN cysteine | ||||||
Experimental info | |||||||||
| Mutagenesis | 91 | 1 | C → A: No FMN binding. Ref.6 | ||||||
| Sequence conflict | 484 | 1 | S → I Ref.1 | ||||||
| Sequence conflict | 484 | 1 | S → I Ref.2 | ||||||
| Sequence conflict | 581 | 1 | S → N Ref.1 | ||||||
| Sequence conflict | 581 | 1 | S → N Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis." Nelson D.C., Lasswell J.E., Rogg L.E., Cohen M.A., Bartel B. Cell 101:331-340(2000) [PubMed: 10847687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Landsberg erecta. |
| [2] | "An Arabidopsis circadian clock component interacts with both CRY1 and phyB." Jarillo J.A., Capel J., Tang R.-H., Yang H.-Q., Alonso J.M., Ecker J.R., Cashmore A.R. Nature 410:487-490(2001) [PubMed: 11260718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| [6] | "FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis." Imaizumi T., Tran H.G., Swartz T.E., Briggs W.R., Kay S.A. Nature 426:302-306(2003) [PubMed: 14628054] [Abstract] Cited for: FUNCTION, INDUCTION, FMN-BINDING AT CYS-91, MUTAGENESIS OF CYS-91. |
| [7] | "Functional conservation of light, oxygen, or voltage domains in light sensing." Cheng P., He Q., Yang Y., Wang L., Liu Y. Proc. Natl. Acad. Sci. U.S.A. 100:5938-5943(2003) [PubMed: 12719523] [Abstract] Cited for: FUNCTION. |
| [8] | "Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH SKP1A; SKP1B; SKP1K; SKP1N AND ADO2. |
| [9] | "Expression and interaction analysis of Arabidopsis Skp1-related genes." Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M. Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK3; ASK11; ASK12 AND ASK13. |
Web resources
| PlantsUBQ A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants |
Cross-references
Sequence databases | |
|---|---|
| AF216523 mRNA. Translation: AAF32298.2. AF252296 mRNA. Translation: AAK27435.1. AC012563 Genomic DNA. Translation: AAG51994.1. AY064999 mRNA. Translation: AAL57647.1. AY113029 mRNA. Translation: AAM47337.1. | |
| IPI | IPI00537296. |
| PIR | F96703. |
| RefSeq | NP_564919.1. |
| UniGene | At.20568 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1N9L based on UniProtKB Q8LPE0. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9C9W9. 16 interactions. |
Genome annotation databases | |
| GeneID | 843133. |
| GenomeReviews | Gene locus AT1G68050 in contig CT485782_GR. |
| KEGG | ath:AT1G68050. |
| NMPDR | fig|3702.1.peg.6256. |
Organism-specific databases | |
| GeneFarm | 5108. 485. |
| TAIR | At1g68050. |
Phylogenomic databases | |
| OMA | Q9C9W9. GRTKILM. |
Gene expression databases | |
| GermOnline | AT1G68050. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001810. F-box. IPR015915. Kelch-typ_b-propeller. IPR006652. Kelch_1. IPR011498. Kelch_2. IPR001610. PAC. IPR000014. PAS. IPR013655. PAS_fold_3. [Graphical view] |
| Gene3D | G3DSA:2.120.10.80. Kelch-typ_b-propeller. 1 hit. |
| Pfam | PF00646. F-box. 1 hit. PF01344. Kelch_1. 2 hits. PF07646. Kelch_2. 3 hits. PF08447. PAS_3. 1 hit. [Graphical view] |
| SMART | SM00086. PAC. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00229. sensory_box. 1 hit. |
| PROSITE | PS50181. FBOX. False negative. PS50113. PAC. False negative. PS50112. PAS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ADO3_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9C9W9 Secondary accession number(s): Q9M648 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


