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Q9C9U4

- MPK15_ARATH

UniProt

Q9C9U4 - MPK15_ARATH

Protein

Mitogen-activated protein kinase 15

Gene

MPK15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191ATPPROSITE-ProRule annotation
    Active sitei216 – 2161Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi96 – 1049ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR

    GO - Biological processi

    1. MAPK cascade Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G73670-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 15 (EC:2.7.11.24)
    Short name:
    AtMPK15
    Short name:
    MAP kinase 15
    Gene namesi
    Name:MPK15
    Ordered Locus Names:At1g73670
    ORF Names:F25P22.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G73670.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Mitogen-activated protein kinase 15PRO_0000245815Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei252 – 2521PhosphothreonineBy similarity
    Modified residuei254 – 2541PhosphotyrosineBy similarity
    Modified residuei257 – 2571PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-252 and Tyr-254, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9C9U4.
    PRIDEiQ9C9U4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9C9U4.

    Interactioni

    Subunit structurei

    Interacts with MKK7.1 Publication

    Protein-protein interaction databases

    IntActiQ9C9U4. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C9U4.
    SMRiQ9C9U4. Positions 54-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini90 – 381292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi252 – 2543TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9C9U4.
    OMAiDHISDAT.
    PhylomeDBiQ9C9U4.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9C9U4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGGGGNLVDG VRRWLFFQRR PSSSSSSNNH DQIQNPPTVS NPNDDEDLKK    50
    LTDPSKLRQI KVQQRNHLPM EKKGIPNAEF FTEYGEANRY QIQEVVGKGS 100
    YGVVGSAIDT HTGERVAIKK INDVFDHISD ATRILREIKL LRLLLHPDVV 150
    EIKHIMLPPS RREFRDVYVV FELMESDLHQ VIKANDDLTP EHHQFFLYQL 200
    LRGLKYVHAA NVFHRDLKPK NILANADCKL KICDFGLARV SFNDAPTAIF 250
    WTDYVATRWY RAPELCGSFF SKYTPAIDIW SVGCIFAEML LGKPLFPGKN 300
    VVHQLDIMTD FLGTPPPEAI SKIRNDKARR YLGNMRKKQP VPFSKKFPKA 350
    DPSALRLLER LIAFDPKDRP SAEEALADPY FNGLSSKVRE PSTQPISKLE 400
    FEFERKKLTK DDIRELIYRE ILEYHPQMLE EYLRGGNQLS FMYPSGVDRF 450
    RRQFAHLEEN QGPGGRSNAL QRQHASLPRE RVPASKNETV EERSNDIERR 500
    TTAAVASTLD SPKASQQAEG TENGGGGGYS ARNLMKSSSI SGSKCIGVQS 550
    KTNIEDSIVE EQDETVAVKV ASLHNS 576

    Note: Inferred from the gene model conservation between members of the family. No experimental confirmation available.

    Length:576
    Mass (Da):65,251
    Last modified:September 5, 2006 - v3
    Checksum:iF4F429EAF3CF4EE7
    GO
    Isoform 2 (identifier: Q9C9U4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         421-431: ILEYHPQMLEE → VMSLLYISSLV
         432-576: Missing.

    Show »
    Length:431
    Mass (Da):49,426
    Checksum:i471AAFF18FD524C4
    GO

    Sequence cautioni

    The sequence AAG52072.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei421 – 43111ILEYHPQMLEE → VMSLLYISSLV in isoform 2. 1 PublicationVSP_020140Add
    BLAST
    Alternative sequencei432 – 576145Missing in isoform 2. 1 PublicationVSP_020141Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012679 Genomic DNA. Translation: AAG52072.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35495.1.
    AF387019 mRNA. Translation: AAK62464.1.
    BT001159 mRNA. Translation: AAN65046.1.
    PIRiG96763.
    RefSeqiNP_565070.2. NM_106026.2. [Q9C9U4-1]
    UniGeneiAt.19296.

    Genome annotation databases

    EnsemblPlantsiAT1G73670.1; AT1G73670.1; AT1G73670. [Q9C9U4-1]
    GeneIDi843702.
    KEGGiath:AT1G73670.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC012679 Genomic DNA. Translation: AAG52072.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35495.1 .
    AF387019 mRNA. Translation: AAK62464.1 .
    BT001159 mRNA. Translation: AAN65046.1 .
    PIRi G96763.
    RefSeqi NP_565070.2. NM_106026.2. [Q9C9U4-1 ]
    UniGenei At.19296.

    3D structure databases

    ProteinModelPortali Q9C9U4.
    SMRi Q9C9U4. Positions 54-427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9C9U4. 1 interaction.

    Proteomic databases

    PaxDbi Q9C9U4.
    PRIDEi Q9C9U4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G73670.1 ; AT1G73670.1 ; AT1G73670 . [Q9C9U4-1 ]
    GeneIDi 843702.
    KEGGi ath:AT1G73670.

    Organism-specific databases

    GeneFarmi 846. 89.
    TAIRi AT1G73670.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9C9U4.
    OMAi DHISDAT.
    PhylomeDBi Q9C9U4.

    Enzyme and pathway databases

    BioCyci ARA:AT1G73670-MONOMER.

    Gene expression databases

    Genevestigatori Q9C9U4.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. Cited for: GENE FAMILY.
    6. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
      Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
      Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK7.

    Entry informationi

    Entry nameiMPK15_ARATH
    AccessioniPrimary (citable) accession number: Q9C9U4
    Secondary accession number(s): Q94EY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3