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Q9C9U4

- MPK15_ARATH

UniProt

Q9C9U4 - MPK15_ARATH

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Protein

Mitogen-activated protein kinase 15

Gene
MPK15, At1g73670, F25P22.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191ATP By similarity
Active sitei216 – 2161Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi96 – 1049ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G73670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 15 (EC:2.7.11.24)
Short name:
AtMPK15
Short name:
MAP kinase 15
Gene namesi
Name:MPK15
Ordered Locus Names:At1g73670
ORF Names:F25P22.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G73670.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Mitogen-activated protein kinase 15PRO_0000245815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei252 – 2521Phosphothreonine By similarity
Modified residuei254 – 2541Phosphotyrosine By similarity
Modified residuei257 – 2571Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-252 and Tyr-254, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C9U4.
PRIDEiQ9C9U4.

Expressioni

Gene expression databases

GenevestigatoriQ9C9U4.

Interactioni

Subunit structurei

Interacts with MKK7.1 Publication

Protein-protein interaction databases

IntActiQ9C9U4. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9C9U4.
SMRiQ9C9U4. Positions 54-427.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 381292Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi252 – 2543TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9C9U4.
OMAiDHISDAT.
PhylomeDBiQ9C9U4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9C9U4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGGGGNLVDG VRRWLFFQRR PSSSSSSNNH DQIQNPPTVS NPNDDEDLKK    50
LTDPSKLRQI KVQQRNHLPM EKKGIPNAEF FTEYGEANRY QIQEVVGKGS 100
YGVVGSAIDT HTGERVAIKK INDVFDHISD ATRILREIKL LRLLLHPDVV 150
EIKHIMLPPS RREFRDVYVV FELMESDLHQ VIKANDDLTP EHHQFFLYQL 200
LRGLKYVHAA NVFHRDLKPK NILANADCKL KICDFGLARV SFNDAPTAIF 250
WTDYVATRWY RAPELCGSFF SKYTPAIDIW SVGCIFAEML LGKPLFPGKN 300
VVHQLDIMTD FLGTPPPEAI SKIRNDKARR YLGNMRKKQP VPFSKKFPKA 350
DPSALRLLER LIAFDPKDRP SAEEALADPY FNGLSSKVRE PSTQPISKLE 400
FEFERKKLTK DDIRELIYRE ILEYHPQMLE EYLRGGNQLS FMYPSGVDRF 450
RRQFAHLEEN QGPGGRSNAL QRQHASLPRE RVPASKNETV EERSNDIERR 500
TTAAVASTLD SPKASQQAEG TENGGGGGYS ARNLMKSSSI SGSKCIGVQS 550
KTNIEDSIVE EQDETVAVKV ASLHNS 576

Note: Inferred from the gene model conservation between members of the family. No experimental confirmation available.

Length:576
Mass (Da):65,251
Last modified:September 5, 2006 - v3
Checksum:iF4F429EAF3CF4EE7
GO
Isoform 2 (identifier: Q9C9U4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     421-431: ILEYHPQMLEE → VMSLLYISSLV
     432-576: Missing.

Show »
Length:431
Mass (Da):49,426
Checksum:i471AAFF18FD524C4
GO

Sequence cautioni

The sequence AAG52072.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei421 – 43111ILEYHPQMLEE → VMSLLYISSLV in isoform 2. VSP_020140Add
BLAST
Alternative sequencei432 – 576145Missing in isoform 2. VSP_020141Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC012679 Genomic DNA. Translation: AAG52072.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35495.1.
AF387019 mRNA. Translation: AAK62464.1.
BT001159 mRNA. Translation: AAN65046.1.
PIRiG96763.
RefSeqiNP_565070.2. NM_106026.2. [Q9C9U4-1]
UniGeneiAt.19296.

Genome annotation databases

EnsemblPlantsiAT1G73670.1; AT1G73670.1; AT1G73670. [Q9C9U4-1]
GeneIDi843702.
KEGGiath:AT1G73670.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC012679 Genomic DNA. Translation: AAG52072.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE35495.1 .
AF387019 mRNA. Translation: AAK62464.1 .
BT001159 mRNA. Translation: AAN65046.1 .
PIRi G96763.
RefSeqi NP_565070.2. NM_106026.2. [Q9C9U4-1 ]
UniGenei At.19296.

3D structure databases

ProteinModelPortali Q9C9U4.
SMRi Q9C9U4. Positions 54-427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9C9U4. 1 interaction.

Proteomic databases

PaxDbi Q9C9U4.
PRIDEi Q9C9U4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G73670.1 ; AT1G73670.1 ; AT1G73670 . [Q9C9U4-1 ]
GeneIDi 843702.
KEGGi ath:AT1G73670.

Organism-specific databases

GeneFarmi 846. 89.
TAIRi AT1G73670.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q9C9U4.
OMAi DHISDAT.
PhylomeDBi Q9C9U4.

Enzyme and pathway databases

BioCyci ARA:AT1G73670-MONOMER.

Gene expression databases

Genevestigatori Q9C9U4.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.
  6. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
    Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
    Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKK7.

Entry informationi

Entry nameiMPK15_ARATH
AccessioniPrimary (citable) accession number: Q9C9U4
Secondary accession number(s): Q94EY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi