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Protein

Mitogen-activated protein kinase 15

Gene

MPK15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119ATPPROSITE-ProRule annotation1
Active sitei216Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 104ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 15 (EC:2.7.11.24)
Short name:
AtMPK15
Short name:
MAP kinase 15
Gene namesi
Name:MPK15
Ordered Locus Names:At1g73670
ORF Names:F25P22.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G73670.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458151 – 576Mitogen-activated protein kinase 15Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphothreonineBy similarity1
Modified residuei254PhosphotyrosineBy similarity1
Modified residuei257PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-252 and Tyr-254, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C9U4.
PRIDEiQ9C9U4.

PTM databases

iPTMnetiQ9C9U4.

Expressioni

Gene expression databases

GenevisibleiQ9C9U4. AT.

Interactioni

Subunit structurei

Interacts with MKK7.1 Publication

Protein-protein interaction databases

IntActiQ9C9U4. 1 interactor.
STRINGi3702.AT1G73670.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C9U4.
SMRiQ9C9U4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 381Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi252 – 254TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C9U4.
OMAiPMEKKGI.
OrthoDBiEOG093605CF.
PhylomeDBiQ9C9U4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C9U4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGGGNLVDG VRRWLFFQRR PSSSSSSNNH DQIQNPPTVS NPNDDEDLKK
60 70 80 90 100
LTDPSKLRQI KVQQRNHLPM EKKGIPNAEF FTEYGEANRY QIQEVVGKGS
110 120 130 140 150
YGVVGSAIDT HTGERVAIKK INDVFDHISD ATRILREIKL LRLLLHPDVV
160 170 180 190 200
EIKHIMLPPS RREFRDVYVV FELMESDLHQ VIKANDDLTP EHHQFFLYQL
210 220 230 240 250
LRGLKYVHAA NVFHRDLKPK NILANADCKL KICDFGLARV SFNDAPTAIF
260 270 280 290 300
WTDYVATRWY RAPELCGSFF SKYTPAIDIW SVGCIFAEML LGKPLFPGKN
310 320 330 340 350
VVHQLDIMTD FLGTPPPEAI SKIRNDKARR YLGNMRKKQP VPFSKKFPKA
360 370 380 390 400
DPSALRLLER LIAFDPKDRP SAEEALADPY FNGLSSKVRE PSTQPISKLE
410 420 430 440 450
FEFERKKLTK DDIRELIYRE ILEYHPQMLE EYLRGGNQLS FMYPSGVDRF
460 470 480 490 500
RRQFAHLEEN QGPGGRSNAL QRQHASLPRE RVPASKNETV EERSNDIERR
510 520 530 540 550
TTAAVASTLD SPKASQQAEG TENGGGGGYS ARNLMKSSSI SGSKCIGVQS
560 570
KTNIEDSIVE EQDETVAVKV ASLHNS
Note: Inferred from the gene model conservation between members of the family. No experimental confirmation available.
Length:576
Mass (Da):65,251
Last modified:September 5, 2006 - v3
Checksum:iF4F429EAF3CF4EE7
GO
Isoform 2 (identifier: Q9C9U4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     421-431: ILEYHPQMLEE → VMSLLYISSLV
     432-576: Missing.

Show »
Length:431
Mass (Da):49,426
Checksum:i471AAFF18FD524C4
GO

Sequence cautioni

The sequence AAG52072 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020140421 – 431ILEYHPQMLEE → VMSLLYISSLV in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_020141432 – 576Missing in isoform 2. 1 PublicationAdd BLAST145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012679 Genomic DNA. Translation: AAG52072.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35495.1.
AF387019 mRNA. Translation: AAK62464.1.
BT001159 mRNA. Translation: AAN65046.1.
PIRiG96763.
RefSeqiNP_565070.2. NM_106026.3. [Q9C9U4-1]
UniGeneiAt.19296.

Genome annotation databases

EnsemblPlantsiAT1G73670.1; AT1G73670.1; AT1G73670. [Q9C9U4-1]
GeneIDi843702.
GrameneiAT1G73670.1; AT1G73670.1; AT1G73670.
KEGGiath:AT1G73670.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012679 Genomic DNA. Translation: AAG52072.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35495.1.
AF387019 mRNA. Translation: AAK62464.1.
BT001159 mRNA. Translation: AAN65046.1.
PIRiG96763.
RefSeqiNP_565070.2. NM_106026.3. [Q9C9U4-1]
UniGeneiAt.19296.

3D structure databases

ProteinModelPortaliQ9C9U4.
SMRiQ9C9U4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9C9U4. 1 interactor.
STRINGi3702.AT1G73670.1.

PTM databases

iPTMnetiQ9C9U4.

Proteomic databases

PaxDbiQ9C9U4.
PRIDEiQ9C9U4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G73670.1; AT1G73670.1; AT1G73670. [Q9C9U4-1]
GeneIDi843702.
GrameneiAT1G73670.1; AT1G73670.1; AT1G73670.
KEGGiath:AT1G73670.

Organism-specific databases

TAIRiAT1G73670.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C9U4.
OMAiPMEKKGI.
OrthoDBiEOG093605CF.
PhylomeDBiQ9C9U4.

Miscellaneous databases

PROiQ9C9U4.

Gene expression databases

GenevisibleiQ9C9U4. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK15_ARATH
AccessioniPrimary (citable) accession number: Q9C9U4
Secondary accession number(s): Q94EY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.