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Q9C9U4 (MPK15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 15

Short name=AtMPK15
Short name=MAP kinase 15
EC=2.7.11.24
Gene names
Name:MPK15
Ordered Locus Names:At1g73670
ORF Names:F25P22.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Subunit structure

Interacts with MKK7. Ref.6

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-252 and Tyr-254, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAG52072.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9C9U4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Inferred from the gene model conservation between members of the family. No experimental confirmation available.
Isoform 2 (identifier: Q9C9U4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     421-431: ILEYHPQMLEE → VMSLLYISSLV
     432-576: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Mitogen-activated protein kinase 15
PRO_0000245815

Regions

Domain90 – 381292Protein kinase
Nucleotide binding96 – 1049ATP By similarity
Motif252 – 2543TXY

Sites

Active site2161Proton acceptor By similarity
Binding site1191ATP By similarity

Amino acid modifications

Modified residue2521Phosphothreonine By similarity
Modified residue2541Phosphotyrosine By similarity
Modified residue2571Phosphothreonine By similarity

Natural variations

Alternative sequence421 – 43111ILEYHPQMLEE → VMSLLYISSLV in isoform 2.
VSP_020140
Alternative sequence432 – 576145Missing in isoform 2.
VSP_020141

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 3.
Checksum: F4F429EAF3CF4EE7

FASTA57665,251
        10         20         30         40         50         60 
MGGGGNLVDG VRRWLFFQRR PSSSSSSNNH DQIQNPPTVS NPNDDEDLKK LTDPSKLRQI 

        70         80         90        100        110        120 
KVQQRNHLPM EKKGIPNAEF FTEYGEANRY QIQEVVGKGS YGVVGSAIDT HTGERVAIKK 

       130        140        150        160        170        180 
INDVFDHISD ATRILREIKL LRLLLHPDVV EIKHIMLPPS RREFRDVYVV FELMESDLHQ 

       190        200        210        220        230        240 
VIKANDDLTP EHHQFFLYQL LRGLKYVHAA NVFHRDLKPK NILANADCKL KICDFGLARV 

       250        260        270        280        290        300 
SFNDAPTAIF WTDYVATRWY RAPELCGSFF SKYTPAIDIW SVGCIFAEML LGKPLFPGKN 

       310        320        330        340        350        360 
VVHQLDIMTD FLGTPPPEAI SKIRNDKARR YLGNMRKKQP VPFSKKFPKA DPSALRLLER 

       370        380        390        400        410        420 
LIAFDPKDRP SAEEALADPY FNGLSSKVRE PSTQPISKLE FEFERKKLTK DDIRELIYRE 

       430        440        450        460        470        480 
ILEYHPQMLE EYLRGGNQLS FMYPSGVDRF RRQFAHLEEN QGPGGRSNAL QRQHASLPRE 

       490        500        510        520        530        540 
RVPASKNETV EERSNDIERR TTAAVASTLD SPKASQQAEG TENGGGGGYS ARNLMKSSSI 

       550        560        570 
SGSKCIGVQS KTNIEDSIVE EQDETVAVKV ASLHNS 

« Hide

Isoform 2 [UniParc].

Checksum: 471AAFF18FD524C4
Show »

FASTA43149,426

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC012679 Genomic DNA. Translation: AAG52072.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35495.1.
AF387019 mRNA. Translation: AAK62464.1.
BT001159 mRNA. Translation: AAN65046.1.
PIRG96763.
RefSeqNP_565070.2. NM_106026.2. [Q9C9U4-1]
UniGeneAt.19296.

3D structure databases

ProteinModelPortalQ9C9U4.
SMRQ9C9U4. Positions 54-427.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9C9U4. 1 interaction.

Proteomic databases

PaxDbQ9C9U4.
PRIDEQ9C9U4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G73670.1; AT1G73670.1; AT1G73670. [Q9C9U4-1]
GeneID843702.
KEGGath:AT1G73670.

Organism-specific databases

GeneFarm846. 89.
TAIRAT1G73670.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9C9U4.
OMADHISDAT.
PhylomeDBQ9C9U4.

Enzyme and pathway databases

BioCycARA:AT1G73670-MONOMER.

Gene expression databases

GenevestigatorQ9C9U4.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK15_ARATH
AccessionPrimary (citable) accession number: Q9C9U4
Secondary accession number(s): Q94EY5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names