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Protein

Mitogen-activated protein kinase 15

Gene

MPK15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119ATPPROSITE-ProRule annotation1
Active sitei216Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 104ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 15 (EC:2.7.11.24)
Short name:
AtMPK15
Short name:
MAP kinase 15
Gene namesi
Name:MPK15
Ordered Locus Names:At1g73670
ORF Names:F25P22.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G73670
TAIRilocus:2027814 AT1G73670

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458151 – 576Mitogen-activated protein kinase 15Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphothreonineBy similarity1
Modified residuei254PhosphotyrosineBy similarity1
Modified residuei257PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-252 and Tyr-254, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C9U4
PRIDEiQ9C9U4

PTM databases

iPTMnetiQ9C9U4

Expressioni

Gene expression databases

ExpressionAtlasiQ9C9U4 baseline and differential
GenevisibleiQ9C9U4 AT

Interactioni

Subunit structurei

Interacts with MKK7.1 Publication

Protein-protein interaction databases

IntActiQ9C9U4, 1 interactor
STRINGi3702.AT1G73670.1

Structurei

3D structure databases

ProteinModelPortaliQ9C9U4
SMRiQ9C9U4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 381Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi252 – 254TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ9C9U4
OMAiGNQLSFM
OrthoDBiEOG093605CF
PhylomeDBiQ9C9U4

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C9U4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGGGNLVDG VRRWLFFQRR PSSSSSSNNH DQIQNPPTVS NPNDDEDLKK
60 70 80 90 100
LTDPSKLRQI KVQQRNHLPM EKKGIPNAEF FTEYGEANRY QIQEVVGKGS
110 120 130 140 150
YGVVGSAIDT HTGERVAIKK INDVFDHISD ATRILREIKL LRLLLHPDVV
160 170 180 190 200
EIKHIMLPPS RREFRDVYVV FELMESDLHQ VIKANDDLTP EHHQFFLYQL
210 220 230 240 250
LRGLKYVHAA NVFHRDLKPK NILANADCKL KICDFGLARV SFNDAPTAIF
260 270 280 290 300
WTDYVATRWY RAPELCGSFF SKYTPAIDIW SVGCIFAEML LGKPLFPGKN
310 320 330 340 350
VVHQLDIMTD FLGTPPPEAI SKIRNDKARR YLGNMRKKQP VPFSKKFPKA
360 370 380 390 400
DPSALRLLER LIAFDPKDRP SAEEALADPY FNGLSSKVRE PSTQPISKLE
410 420 430 440 450
FEFERKKLTK DDIRELIYRE ILEYHPQMLE EYLRGGNQLS FMYPSGVDRF
460 470 480 490 500
RRQFAHLEEN QGPGGRSNAL QRQHASLPRE RVPASKNETV EERSNDIERR
510 520 530 540 550
TTAAVASTLD SPKASQQAEG TENGGGGGYS ARNLMKSSSI SGSKCIGVQS
560 570
KTNIEDSIVE EQDETVAVKV ASLHNS
Note: Inferred from the gene model conservation between members of the family. No experimental confirmation available.
Length:576
Mass (Da):65,251
Last modified:September 5, 2006 - v3
Checksum:iF4F429EAF3CF4EE7
GO
Isoform 2 (identifier: Q9C9U4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     421-431: ILEYHPQMLEE → VMSLLYISSLV
     432-576: Missing.

Show »
Length:431
Mass (Da):49,426
Checksum:i471AAFF18FD524C4
GO

Sequence cautioni

The sequence AAG52072 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020140421 – 431ILEYHPQMLEE → VMSLLYISSLV in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_020141432 – 576Missing in isoform 2. 1 PublicationAdd BLAST145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012679 Genomic DNA Translation: AAG52072.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35495.1
AF387019 mRNA Translation: AAK62464.1
BT001159 mRNA Translation: AAN65046.1
PIRiG96763
RefSeqiNP_565070.2, NM_106026.3 [Q9C9U4-1]
UniGeneiAt.19296

Genome annotation databases

EnsemblPlantsiAT1G73670.1; AT1G73670.1; AT1G73670 [Q9C9U4-1]
GeneIDi843702
GrameneiAT1G73670.1; AT1G73670.1; AT1G73670 [Q9C9U4-1]
KEGGiath:AT1G73670

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMPK15_ARATH
AccessioniPrimary (citable) accession number: Q9C9U4
Secondary accession number(s): Q94EY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 5, 2006
Last modified: April 25, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health