Reviewed, UniProtKB/Swiss-Prot Q9C9U2 (CDKD1_ARATH)

Names and origin

Protein names

Recommended name:
    Cyclin-dependent kinase D-1
      Short name=CDKD;1
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    CDK-activating kinase 3-At
      Short name=CAK3-At

Gene names

Name:	CDKD-1
Ordered Locus Names:	At1g73690
ORF Names:	F25P22.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus. Ref.5
Tissue specificity	Expressed at low levels in suspension cell culture, but not in plant organs. Ref.1
Post-translational modification	Autophosphorylated.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Traceable author statement. Source: TAIR protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of cell cycle Traceable author statement. Source: TAIR
Cellular component	nucleus Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Cyclin-dependent kinase D-1		PRO_0000293119
Regions
	Domain	11 – 291	281	Protein kinase
	Nucleotide binding	17 – 25	9	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Binding site	40	1	ATP Probable
Amino acid modifications
	Modified residue	21	1	Phosphothreonine By similarity
	Modified residue	22	1	Phosphotyrosine By similarity
	Modified residue	160	1	Phosphoserine By similarity
	Modified residue	166	1	Phosphothreonine By similarity
Experimental info
	Mutagenesis	40	1	K → R: Prevents autophosphorylation. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9C9U2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 32BA95E61E6D2C45	FASTA	398	45,138
	10 20 30 40 50 60 MEQPKKVADR YLKREVLGQG TYGVVFKATD TKNGETVAIK KIRLGKEKEG VNVTALREIK 70 80 90 100 110 120 LLKELKHPHI IELIDAFPHK ENLHIVFEFM ETDLEAVIRD RNLYLSPGDV KSYLQMILKG 130 140 150 160 170 180 LEYCHGKWVL HRDMKPNNLL IGPNGQLKLA DFGLARIFGS PGRKFTHQVF ARWYRAPELL 190 200 210 220 230 240 FGAKQYDGAV DVWAAGCIFA ELLLRRPFLQ GNSDIDQLSK IFAAFGTPKA DQWPDMICLP 250 260 270 280 290 300 DYVEYQFVPA PSLRSLLPTV SEDALDLLSK MFTYDPKSRI SIQQALKHRY FTSAPSPTDP 310 320 330 340 350 360 LKLPRPVSKQ DAKSSDSKLE AIKVLSPAHK FRRVMPDRGK SGNGFKDQSV DVMRQASHDG 370 380 390 QAPMSLDFTI LAERPPNRPT ITSADRSHLK RKLDLEFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana." Shimotohno A., Matsubayashi S., Yamaguchi M., Uchimiya H., Umeda M. FEBS Lett. 534:69-74(2003) [PubMed: 12527363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed: 11971144] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis." Shimotohno A., Umeda-Hara C., Bisova K., Uchimiya H., Umeda M. Plant Cell 16:2954-2966(2004) [PubMed: 15486101] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-40.
[6]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed: 17094738] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047275 mRNA. Translation: BAB62844.1. AC012679 Genomic DNA. Translation: AAG52081.1. AY063843 mRNA. Translation: AAL36199.1. AY091227 mRNA. Translation: AAM14166.1.
IPI	IPI00547478.
PIR	A96764.
RefSeq	NP_177510.1.
UniGene	At.17996
3D structure databases
HSSP	HSSP built from PDB template 1P2A based on UniProtKB P24941.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C9U2.
Genome annotation databases
GeneID	843704.
GenomeReviews	Gene locus AT1G73690 in contig CT485782_GR.
KEGG	ath:AT1G73690.
NMPDR	fig|3702.1.peg.6873.
Organism-specific databases
GeneFarm	3285. 109.
TAIR	At1g73690.
Phylogenomic databases
OMA	VFSSKDQ.
Enzyme and pathway databases
BRENDA	2.7.11.22. 302. 2.7.11.23. 302.
Gene expression databases
ArrayExpress	Q9C9U2.
Genevestigator	Q9C9U2.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKD1_ARATH

Accession

Primary (citable) accession number: Q9C9U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)